Abstract
A full-length double-stranded DNA copy of the single-stranded circular DNA associated with coconut foliar decay virus (CFDV) was constructed. Full-length CFDV DNA and smaller fragments were transcriptionally fused to the β-glucuronidase reporter gene and examined for promoter activity in vivo. In stably transformed tobacco plants, the CFDV DNA promoter confered a tissue-specific expression pattern in that the reporter gene was specifically expressed in the phloem tissue of the vascular system in stem, leaves and flower. These results are in agreement with the previously reported association of CFDV DNA with the phloem of its coconut host plant.
This is a preview of subscription content, log in via an institution to check access.
References
Bevan MW: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).
Hanold D, Langridge P, Randels JW: The use of cloned sequences for the identification of coconut foliar decay disease-associated DNA. J Gen Virol 69: 1323–1329 (1988).
Harding RM, Burns TM, Hafner G, Dietzgen RG, Dale JL: Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. J Gen Virol 74: 323–328 (1993).
Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
Lam E, Chua NH: ASF-2: A factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters. Plant Cell 1: 1147–1156 (1989).
Lam E, Benfey PN, Gilmartin PM, Fang R, Chua NH: Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proc Natl Acad Sci USA 86: 7890–7894 (1989).
Medberry SL, Lockhart BEL, Olszewski NE: Properties of Commelina yellow mottle virus's complete DNA sequence, genomic discontinuities and transcript suggest that it is a pararetrovirus. Nucl Acids Res 18: 5505–5513 (1990).
Medberry SL, Lockhart BEL, Olszewski NE: The Commelina yellow mottle virus promoter is a strong promoter in vascular and reproductive tissues. Plant Cell 4: 185–192 (1992).
Randles JW, Hanold D: Coconut foliar decay virus particles are 20 nm icosahedra. Intervirology 30: 177–180 (1989).
Randles JW, Julia JF, Calvez C, Dollet M: Association of a single-stranded DNA with foliar decay disease of coconut palm in Vanuatu. Phytopathology 26: 889–894 (1986).
Randles JW, Miller DC, Morin JP, Rohde W, Hanold D: Localisation of coconut foliar decay virus in coconut palm. Ann Appl Biol 121: 601–617 (1992).
Rohde W, Randles JW, Langridge P, Hanold D: Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology 176: 648–651 (1990).
Tacke E, Prüfer D, Salamini F, Rohde W: Characterization of a potato leafroll luteovirus subgenomic RNA: differential expression by internal translation initiation and UAG suppression. J Gen Virol 71: 2265–2272 (1990).
Töpfer R, Matzeit V, Gronenborn B, Schell J, Steinbiss HH: A set of plant expression vectors for transcriptional and translational fusions. Nucl Acids Res 15: 5890 (1987).
Wissenbach M, Überlacker B, Vogt F, Becker D, Salamini F, Rohde W: Myb genes from Hordeum vulgare: tissue-specific expression of chimeric Myb promoter/Gus genes in transgenic tobacco. Plant J 4: 411–422 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rohde, W., Becker, D. & Randles, J.W. The promoter of coconut foliar decay-associated circular single-stranded DNA directs phloem-specific reporter gene expression in transgenic tobacco. Plant Mol Biol 27, 623–628 (1995). https://doi.org/10.1007/BF00019328
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00019328