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A molecular study of dormancy breaking and germination in seeds of Trollius ledebouri

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Abstract

A cDNA library was generated from seeds of Trollius ledebouri cv. Golden Queen after GA3 treament. Five clones encoded mRNAs which were down-regulated during dormancy breaking and the initial stages of germination. Two of these showed homology to storage proteins (pPCB3 and pPCB4) and one each to the late-embryogenesis-abundant (LEA) group 2 dehydrin proteins (pPCB2), a barley glucose dehydrogenase (pPCB6) and the glutathione S-transferase (GST) superfamily (pPCB7). Transcript levels declined over 8 days in GA3-treated seeds. In dormant imbibed seeds transcript levels were relatively unchanged over the same period except for the PCB3 transcript, the level of which increased.

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  1. Paul C. Bailey

    Present address: Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB, Oxford, UK

Authors and Affiliations

  1. Department of Physiology and Environmental Science, Faculty of Agricultural and Food Sciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, Leicestershire, UK

    Paul C. Bailey, Grantley W. Lycett & Jeremy A. Roberts

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  1. Paul C. Bailey
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  2. Grantley W. Lycett
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  3. Jeremy A. Roberts
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Bailey, P.C., Lycett, G.W. & Roberts, J.A. A molecular study of dormancy breaking and germination in seeds of Trollius ledebouri . Plant Mol Biol 32, 559–564 (1996). https://doi.org/10.1007/BF00019110

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  • DOI: https://doi.org/10.1007/BF00019110

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