Skip to main content
SpringerLink
Log in

Aquatic microflora enumeration by means of adenylic nucleotides dosage

  • Published:
Hydrobiologia Aims and scope Submit manuscript

Abstract

A good parallelism exists between bacterial enumeration on an agar medium and the pool quantity determination of adenylic nucleotides. The energetic charge is an important parameter to evaluate the metabolic activity of microorganisms. After microscopic verification, it has been shown that different filtrations allow to separate and to count the various populations (phytoplankton, zooplankton, bacteria) by means of ATP content. The development cycle of microorganisms in a lake has been followed. Primary production of the micro-biomass is easily calculated by the variation of ATP content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Champiat, D. 1977. Echange de nucléotides adényliques et croissance bactérienne. C. R. Soc. Biol. 171: 54–59.

    CAS  Google Scholar 

  • Gendraud, M. 1975. Quelques aspects du métabolisme des acides ribonucléiques et des nucléotides libres en relation avec l'édification et la dormance des tubercules de Topinambour (Helianthus tuberosus L.). Thèse d'Etat, Clermont-Ferrand.

  • Kuznetsov, S. I. 1968. Recent studies on the role of microorganisms in the cycling of substances in lakes. Limnol. and Oceanog., 13: 211–244.

    Article  CAS  Google Scholar 

  • Kuznetsov, S. I. & Romanenko, W. I. 1966. Produktion der Biomasse heterotropher Bakterien und die Geschwindigkeit ihrer Vermehrung in Rybinsk Stanne. Verh. Int. Verein. Limnol., 16: 1493–1500.

    Google Scholar 

  • Pradet, A. 1967. Etude des adénosines 5′ mono, di et triphosphates dans les tissus végétaux. I. Dosage enzymatique. Physiol. vég., 5: 209–221.

    CAS  Google Scholar 

  • Strehler, B. L. 1968. Bioluminescence assay: Principles and Practice. In Methods of biochemical analysis, vol. 16, 99–181, Interscience Publ. New-York.

    Chapter  Google Scholar 

  • Vollenweider, R. A. 1971. Primary production in aquatic environments. IBP Handbook n° 12, Blackwell Scientific Publications, Oxford, London and Edinburgh, 232 p.

    Google Scholar 

  • Wurtz, A. 1946. Rôle et importance des bactéries dans l'eau. Bull. fr. Pisc., 143: 80–88.

    Article  Google Scholar 

  • Wurtz, A. 1947. Rôle et importance des bactéries dans l'eau. Bull. Fr. Pisc., 144: 144–148.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Microbiologie, 4 rue Ledru, 63000, Clermont Ferrand, France

    D. Champiat & J. P. Larpent

Authors
  1. D. Champiat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. P. Larpent
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Champiat, D., Larpent, J.P. Aquatic microflora enumeration by means of adenylic nucleotides dosage. Hydrobiologia 58, 37–42 (1978). https://doi.org/10.1007/BF00018893

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00018893

Keywords

Search

Navigation