Abstract
In order to identify the domain within Photosystem II complexes that functions in the evolution of oxygen, we performed limited proteolysis with lysylendopeptidase of the core complex of Photosystem II which had been depleted of the extrinsic 33-kDa protein (Mn-stabilizing protein). The cleavage sites were estimated from the amino-terminal sequences of the degradation fragments, their apparent molecular masses and amino-acid compositions. Under certain conditions, the D2 protein was cleaved at Lys13; and a chlorophyll a-binding protein, CP 47, was cleaved at Lys227 and Lys389. Another chlorophyll a-binding protein, CP 43, was degraded more rapidly than CP 47. The oxygen-evolving activity and the capacity for rebinding of the 33-kDa protein to the core complex of Photosystem II decreased in parallel, with kinetics very similar to those of the cleavage of CP 47 at Lys389. These observations strongly suggest that the hydrophilic domain around Lys389 of CP 47, which are located on the lumenal side, is important in the binding of the 33-kDa protein and in maintaining the oxygen-evolving activity of the Photosystem II complex.
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Abbreviations
- CP 47 and CP 43-:
-
intrinsic chlorophyll a-binding proteins with apparent molecular masses of 47 and 43 kDa, respectively
- PBQ-:
-
phenyl-p-benzoquinone
- TLCK-:
-
N-α-p-tosyl-L-lysine chloromethyl ketone
References
Alt J, Morris J, Westhoff P and Herrmann RG (1984) Nucleotide sequence of the clustered genes for the 44 kd chlorophyll a apoprotein and the ‘32 kd’-like protein of the Photosystem II reaction center in the spinach plastid chromosome. Curr Genet 8: 597–606
Amesz J (1983) The role of manganese in photosynthetic oxygen evolution. Biochim Biophys Acta 726: 1–12
Bricker TM (1990) The structure and function of CPa-1 and CPa-2 in Photosystem II. Photosynthe Res 24: 1–13
Bricker TM and Frankel LK (1987) Use of a monoclonal antibody in structural investigations of the 49-kDa polypeptide of Photosystem II. Arch Biochem Biophys 256: 295–301
Bricker TM, Odom WR and Queirolo CB (1988) Close association of the 33 kDa extrinsic protein with the apoprotein of CPa1 in Photosystem II. FEBS Lett 231: 111–117
Eaton-Rye JJ and Vermaas WFJ (1991) Oligonucleotide-directed mutagenesis of psbB, the gene encoding CP 47, employing a deletion mutant strain of the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol Biol 17: 1165–1177
Enami I, Satoh K and Katoh S (1987) Crosslinking between the 33 kDa extrinsic protein and the 47 kDa chlorophyll-carrying protein of the PS II reaction center core complex. FEBS Lett 226: 161–165
Frankel LK and Bricker TM (1989) Epitope mapping of the monoclonal antibody FAC2 on the apoprotein of CPa-1 in Photosystem II. FEBS Lett 257: 279–282
Ghanotakis DF and Yocum CF (1990) Photosystem II and the oxygen-evolving complex. Annu Rev Plant Physiol Plant Mol Biol 41: 255–276
Ghanotakis DF, Demetriou DM and Yocum CF (1987) Isolation and characterization of an oxygen-evolving Photosystem II reaction center core preparation and a 28 kDa Chl-a-binding protein. Biochim Biophys Acta 891: 15–21
Hansson Ö and Wydrzynski T (1990) Current perceptions of Photosystem II. Photosynth Res 23: 131–162
Herrmann RG, Alt J, Schiller B, Widger WR and Cramer WA (1984) Nucleotide sequence of the gene for apocytochrome b-559 on the spinach plastid chromosome: Implications for the structure of the membrane protein. FEBS Lett 176: 239–244
Holschuh K, Bottomley W and Whitfeld PR (1984) Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of Photosystem II and for tRNASer (UGA). Nucl Acids Res 12: 8819–8834
Ikeuchi M and Inoue Y (1986) Characterization of O2 evolution by a wheat Photosystem II reaction center complex isolated by a simplified method: Disjunction of secondary acceptor quinone and enhanced Ca2+ demand. Arch Biochem Biophys 247: 97–107
Ikeuchi M, Yuasa M and Inoue Y (1985) Simple and discrete isolation of an O2-evolving PS II reaction center complex retaining Mn and the extrinsic 33 kDa protein. FEBS Lett 185: 316–322
Isogai Y, Yamamoto Y and Nishimura M (1985) Association of the 33-kDa polypeptide with the 43-kDa component in Photosystem II particles. FEBS Lett 187: 240–244
Kuwabara T and Murata N (1982) Inactivation of photosynthetic oxygen evolution and concomitant release of three polypeptides in the Photosystem II particles of spinach chloroplasts. Plant Cell Physiol 23: 533–539
Kuwabara T, Miyao M, Murata T and Murata N (1985) The function of 33-kDa protein in the photosynthetic oxygen-evolution system studied by reconstitution experiments. Biochim Biophys Acta 806: 283–289
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Michel H, Hunt DF, Shabanowitz J and Bennett J (1988) Tandem mass spectrometry reveals that three Photosystem II proteins of spinach chloroplasts contain N-acetyl-O-phosphothreonine at their NH2 termini. J Biol Chem 263: 1123–1130
Miyao M and Murata N (1984) Role of the 33-kDa poly-peptide in preserving Mn in the photosynthetic oxygen-evolution system and its replacement by chloride ions. FEBS Lett 170: 350–354
Miyao M, Murata N, Lavorel J, Maison-Peteri B, Boussac A and Etienne A-L (1987) Effect of the 33-kDa protein on the S-state transitions in photosynthetic oxygen evolution. Biochim Biophys Acta 890: 151–159
Moos MJr, Nguyen NY and Liu T-Y (1988) Reproducible high yield sequencing of proteins electrophoretically separated and transferred to an inert support. J Biol Chem 263: 6005–6008
Morris J and Herrmann RG (1984) Nucleotide sequence of the gene for the P680 chlorophyll a apoprotein of the Photosystem II reaction center from spinach. Nucl Acids Res 12: 2837–2850
Odom WR and Bricker TM (1992) Interaction of CPa-1 with the manganese-stabilizing protein of Photosystem II: Identification of domains cross-linked by 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide. Biochemistry 31: 5616–5620
Tae G-S and Cramer WA (1989) Lumen-side topography of the α-subunit of the chloroplast cytochrome b-559. FEBS Lett 259: 161–164
Tang X-S and Satoh K (1985) The oxygen-evolving Photosystem II core complex. FEBS Lett 179: 60–64
Trebst A (1986) The topology of the plastoquinone and herbicide binding peptides of Photosystem II in the thylakoid membrane. Z Naturforsch 41c: 240–245
vanLeeuwen PJ, Vos MH and vanGorkom HJ (1990) Photosynthetic oxygen evolution. Biochim Biophys Acta 1018: 173–176
Zurawski G, Bohnert HJ, Whitfeld PR and Bottomley W (1982) Nucleotide sequence of the gene for the Mr 32 000 thylakoid membrane protein from Spinacia oleracea and Nicotiana debneyi predicts a totally conserved primary translation product of Mr 38 950. Proc Natl Acad Sci USA 79: 7699–7703
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Hayashi, H., Fujimura, Y., Mohanty, P.S. et al. The role of CP 47 in the evolution of oxygen and the binding of the extrinsic 33-kDa protein to the core complex of Photosystem II as determined by limited proteolysis. Photosynth Res 36, 35–42 (1993). https://doi.org/10.1007/BF00018073
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DOI: https://doi.org/10.1007/BF00018073