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Sequence and tissue-specific expression of a putative peroxidase gene from wheat (Triticum aestivum L.)

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Abstract

We have used a cDNA clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic libary of wheat (Triticum aestivum L. cv. Cheyenne) and isolated one positive clone, lambda POX1. Sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pI of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (Brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. The two introns in the wheat gene are at the same positions as introns in the peroxidase genes of tomato and horseradish. Results of S1-mapping experiments suggest that this gene is neither pathogen-nor wound-induced in leaves but is constitutively expressed in roots.

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Authors and Affiliations

  1. Institute for Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008, Zürich, Switzerland

    Cecilia Hertig, Gabriela Rebmann, John Bull, Felix Mauch & Robert Dudler

Authors
  1. Cecilia Hertig
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  2. Gabriela Rebmann
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  3. John Bull
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  4. Felix Mauch
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  5. Robert Dudler
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Hertig, C., Rebmann, G., Bull, J. et al. Sequence and tissue-specific expression of a putative peroxidase gene from wheat (Triticum aestivum L.). Plant Mol Biol 16, 171–174 (1991). https://doi.org/10.1007/BF00017928

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  • DOI: https://doi.org/10.1007/BF00017928

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