Abstract
The localization of acid and alkaline phosphatases in the intraradical hyphae of the arbuscular mycorrhizal fungi, Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe (Gm), Gl. etunicatum Becker and Gerd. (Ge) and Gigaspora rosea Nicol. and Schenck (Gir) were compared. Marigold (Tagetes patula L.) and leek (Allium porrum L.) were inoculated with each of the three fungi. The mycorrhizal roots were harvested at 3, 4, 5 and 6 weeks after sowing (WAS), treated with a digestion solution containing cellulase and pectinase, and then stained for phosphatase activities at pH 5.0 and pH 8.5. The development of fungal structures in the host root was also examined. Gm formed fine-branched (mature) arbuscules only at the early phase of infection (3 to 4 WAS). Mature arbuscules of Ge and Gir were observed from the early phase (4 WAS) up to the end of experiment. At pH 5.0, the localization of the phosphatase activities of the three fungi were similar irrespective to host plant species. The activity appeared in mature arbuscules and intercellular hyphae, whereas the collapsed arbuscules were inactive. Ten millimolar NaF, an acid phosphatase inhibitor, inhibited the phosphatase activities of Gm and Ge but did not affect that of Gir. At pH 8.5, a difference among the fungal species was found in the localization of phosphatase activity while that between host species was not. The mature arbuscules were also the active sites in all three species. Only Gir showed the activity in the intercellular hyphae while the two Glomus spp. did not. Five millimolar EDTA inhibited the activity of Gir at pH 8.5 while the activities of Ge and Gm were not affected by either 5 mM EDTA or 10 mM KCN (both are alkaline phosphatase inhibitors).
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References
Arnold W N, Sakai K H and Mann L C 1987 Selective inactivation of an extra-cytoplasmic acid phosphatase of yeast-like cells of Sporothix schenckii by sodium fluoride. J. Gen. Microbiol. 133, 1503–1509.
Belle H V 1972 Kinetics and inhibition of alkaline phosphatases from canine tissues. Biochim. Biophys. Acta 289, 158–168.
Ezawa T and Yoshida T 1994a Characterization of phosphatase in marigold roots infected with vesicular-arbuscular mycorrhizal fungi. Soil Sci. Plant Nutr. 40, 255–264.
Ezawa T and Yoshida T 1994b Acid phosphatase specific to arbuscular mycorrhizal infection in marigold and possible role in symbiosis. Soil Sci. Plant Nutr. 40, 655–665.
Gianinazzi S, Gianinazii-Pearson V and Dexheimer J 1979 Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza. III. Ultrastructural localization of acid and alkaline phosphatase in onion roots infected by Glomus mosseae (Nicol. and Gerd.). New Phytol. 82, 127–132.
Gianinazzi-Pearson V and Gianinazzi S 1978 Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza. II. Soluble alkaline phosphatase specific to mycorrhizal infection in onion roots. Physiol. Plant Pathol. 12, 45–53.
Hepper C M, Sen R and Maskall C S 1986 Identification of vesicular arbuscular mycorrhizal fungi in roots of leek (Allium porrum L.) and maize (Zea mays L.) on the basis of enzyme mobility during polyacrylamide gel electrophoresis. New Phytol. 102, 529–539.
Macdonald R M and Lewis M 1978 The occurrence of some acid phosphatases and dehydrogenases in the vesicular-arbuscular mycorrhizal fungus Glomus mosseae. New Phytol. 80, 135–141.
McGee P A and Smith S E 1990 Activity of succinate dehydrogenase in vesicular-arbuscular mycorrhizal fungi after enzymic digestion from roots of Allium porrum. Mycol. Res. 94, 305–308.
Morton J B and Benny G L 1990 Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): a new order, Glomales, two new suborders, Glominae and Gigasporinae, and two new families, Acaulosporaceae and Gigasporaceae, with an emendation of Glomaceae. Mycotaxon 37, 471–491.
Pearson J N and Jakobsen I 1993 Symbiotic exchange of carbon and phosphorus between cucumber and three arbuscular mycorrhizal fungi. New Phytol. 124, 481–488.
Saito M 1995 Enzyme activities of the internal hyphae and germinated spores of an arbuscular mycorrhizal fungus, Gigaspora margarita (Becker and Hall). New Phytol. 129, 425–431.
Simon L, Bousquet J, Lévesque R C and Lalonde M 1993 Origin and diversification of endomycorrhizal fungi and coincidence with vascular land plants. Nature 363, 67–69.
Smith S E, Dickson S, Morris C and Smith F A 1994 Transfer of phosphate from fungus to plant in VA mycorrhizas: calculation of the area of symbiotic interface and of fluxes of P from two different fungi to Allium porrum L. New Phytol. 127, 93–99.
Smith S E, Stjohn B J, Smith F A and Nicholas D J D 1985 Activity of glutamine synthetase and glutamete dehydrogenase in Trifolium subterraneum L. and Allium cepa L: effects of mycorrhizal infection and phosphate nutrition. New Phytol. 99, 211–227.
Tisserant B, Gianinazzi-Pearson V, Gianinazzi S and Gollotte A 1993 In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections. Mycol. Res. 97, 245–250.
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Ezawa, T., Saito, M. & Yoshida, T. Comparison of phosphatase localization in the intraradical hyphae of arbuscular mycorrhizal fungi, Glomus spp. and Gigaspora spp.. Plant Soil 176, 57–63 (1995). https://doi.org/10.1007/BF00017675
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DOI: https://doi.org/10.1007/BF00017675