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The extrinsic 33 kDa polypeptide of the oxygen-evolving complex of photosystem II is a putative calcium-binding protein and is encoded by a multi-gene family in pea

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Abstract

The extrinsic 33 kDa polypeptide of the water-oxidizing complex has been extracted from pea photosystem II particles by washing with alkaline-Tris and purified by ion-exchange chromatography. The N-terminal amino acid sequence has been determined, and specific antisera have been raised in rabbits and used to screen a pea leaf cDNA library in λgt11. Determination of the nucleotide sequence of positive clones revealed an essentially full-length cDNA for the 33 kDa polypeptide, the deduced amino acid sequence showing it to code for a mature protein of 248 amino acids with an N-terminal transit peptide of 81 amino acids. The protein showed a high degree of conservation with previously reported sequences for the 33 kDa protein from other species and the sequence contained a putative Ca2+-binding site with homology to mammalian intestinal calcium-binding proteins. Northern analysis of total pea RNA indicated a message of approximately 1.4 kb, in good agreement with the size of the cDNA obtained at 1.3 kbp. Southern blots of genomic DNA probed with the labelled cDNA give rise to several bands suggesting that the 33 kDa polypeptide is coded by a multi-gene family.

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Abbreviations

ATZ -:

anilinothiazolinone

DITC -:

p-phenylenediisothiocyanate

PTH -:

phenylthiohydantoin

TFA -:

trifluoroacetic acid

Tris -:

tris (hydroxymethyl) aminomethane

bis-Tris -:

bis (2-hydroxyethyl) imino-tris (hydroxymethyl)-methane

p.f.u. -:

plaque-forming units

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Author notes

  1. Barbara J. Newman

    Present address: National Institute of Medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, UK

Authors and Affiliations

  1. Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK

    Richard Wales, Barbara J. Newman & John C. Gray

  2. Department of Biochemistry, University of Leeds, LS2 9JT, Leeds, UK

    Darryl Pappin

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Wales, R., Newman, B.J., Pappin, D. et al. The extrinsic 33 kDa polypeptide of the oxygen-evolving complex of photosystem II is a putative calcium-binding protein and is encoded by a multi-gene family in pea. Plant Mol Biol 12, 439–451 (1989). https://doi.org/10.1007/BF00017583

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