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Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota

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Abstract

Molecular cloning of dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carota was achieved by immunoscreening of a cDNA library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. Comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of DHFR and TS thus confirming the bifunctional nature of the carrot protein. As in other organisms, a higher degree of conservation was observed in the TS domain. Analysis of the dhfr-ts gene content in carrot revealed the presence of several copies per diploid genome.

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Authors and Affiliations

  1. Dipartimento di Genetica e Microbiologia, Sezione di Microbiologia e Fisiologia Vegetale, Università di Pavia, via Abbiategrasso 207, 27100, Pavia, Italy

    Meizhong Luo, Pietro Piffanelli, Luca Rastelli & Rino Cella

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  1. Meizhong Luo
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  2. Pietro Piffanelli
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  3. Luca Rastelli
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  4. Rino Cella
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Luo, M., Piffanelli, P., Rastelli, L. et al. Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota . Plant Mol Biol 22, 427–435 (1993). https://doi.org/10.1007/BF00015973

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  • DOI: https://doi.org/10.1007/BF00015973

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