Skip to main content
SpringerLink
Log in

Sequence analysis of three fragments of maize nuclear DNA which replicate autonomously in yeast

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

There maize nuclear DNA fragments were isolated on the basis of their ability to confer replication on chimeric plasmids in yeast. These Eco RI fragments of 2.5, 2.8 and 5.5 kb are repeated elements within the maize genome. The 2.5 and 2.8 kb fragments represent a family of elements repeated 11 000 times in the maize haploid genome, while the 5.5 kb fragment is part of another family of 28 000 elements. These fragments were subcloned to further define the unique region of ARS activity. The sequence of each 550–650 bp ARS subclone is reported here, and compared to the flanking regions which do not show ARS activity. The ARS elements are 65–70% A+T as compared to 50–55% for the maize genome as a whole. There is approximately 15% sequence divergence, as well as variation of ARS efficiency, among family members. ARS subclones contain the proposed yeast consensus sequence.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Berlani RE, Davis RW, Walbot V: Genomic organization of two families of highly repeated nuclear DNA sequences of maize selected for autonomous replicating activity in yeast. Plant Mol Biol 11: 161–172 (1988).

    Google Scholar 

  2. Broach JR, Li YY, Feldman J, Jayaram M, Abraham J, Nasmyth KA, Hicks JB: Localization and sequence analysis of yeast origins of DNA replication. Cold Spring Harbor Symp Quant Biol 47: 1165–1173 (1982).

    Google Scholar 

  3. Brown PC, Tlsty TD, Schmike RT: Enhancement of methotrexate resistance and dihydrofolate reductase gene amplification by treatment of mouse 3T6 cells with hydroxyurea. Mol Cell Biol 3: 1097–1107 (1983).

    Google Scholar 

  4. Celniker SE, Campbell JL: Yeast DNA replication in vitro: Initiation and elongation events mimic in vivo processes. Cell 31: 201–213 (1982).

    Google Scholar 

  5. Celniker SE, Sweder D, Srienc F, Bailey JE, Campbell JL: Deletion mutations affecting autonomously replicating sequence ARS1 in Saccharomyces cerevisiae. Mol Cell Biol 4: 2455–2466 (1982).

    Google Scholar 

  6. Davis RW, Botstein D, Roth JR: A Manual for Genetic Engineering, Advanced Bacterial Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1980).

    Google Scholar 

  7. Fangman W, Rice RH, Chlebowitz-Sledziewska E: ARS replication during the yeast S phase. Cell 32: 831–838 (1983).

    Google Scholar 

  8. Hake S, Walbot V: The genome of Zea mays, its organization and homology to related grasses. Chromosoma (Berl.) 79: 251–270 (1980).

    Google Scholar 

  9. Hieter P, Mann C, Snyder M, Davis RW: Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss. Cell 40: 381–392 (1985).

    Google Scholar 

  10. Kearsey S: Structural requirements for the function of a yeast chromosomal replicator. Cell 37: 299–307 (1984).

    Google Scholar 

  11. Kunkel GR, Martinson HG: Nucleosomes will not form on double stranded RNA or over poly(dA) poly(dT) tracts in recombinant DNA. Nucl Acids Res 9: 6869–6888 (1981).

    Google Scholar 

  12. Marunouchi T, Hosoya H: Isolation of an autonomously replicating sequence (ARS) from satellite DNA of Drosophila melanogaster. Mol Gen Genet 196: 258–265 (1984).

    Google Scholar 

  13. Maxam AM, Gilbert W: Sequencing of end labeled DNA with base specific chemical cleavage. Methods in Enzymology 65: 499–560 (1980).

    Google Scholar 

  14. Messing J: New M13 vectors for cloning. In: Wu R, Grossman L, Moldave K (eds) Methods in Enzymology 101 (Part C) Recombinant DNA Techniques, pp. 20–78. Academic Press, New York (1983).

    Google Scholar 

  15. Ohtani T, Uchimiya H, Kato A, Harada H, Sugita M, Sugiura M: Location and nucleotide sequence of a tobacco chloroplast DNA segment capable of replication in yeast. Mol Gen Genet 195: 1–4 (1984).

    Google Scholar 

  16. Ohtani T, Kiyokawa S, Ohgawara T, Harada H, Uchimiya H: Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast. Plant Mol Biol 5: 35–40 (1985).

    Google Scholar 

  17. Overbeeke N, Haring MA, John H, Nijkamp JJ, Kool AJ: Cloning of Petunia hybrida chloroplast DNA sequences capable of autonomous replication in yeast. Plant Mol Biol 3: 235–241 (1984).

    Google Scholar 

  18. Rigby PWJ, Dieckman M, Rhodes C, Berg P: Labeling deoxyribonucleic acid to high specific activity in vitro by nicktranslation with DNA polymerase I. J Mol Biol 113: 237–251 (1977).

    Google Scholar 

  19. Rivin CJ, Cullis CA, Walbot V: Evaluating quantitative variation in the genome of Zea mays. Genetics 113: 1009–1019 (1986).

    Google Scholar 

  20. Rochaix J-D, vanDillewijn J, Rahire M: Construction and characterization of autonomously replicating plasmids in the green unicellular alga Chlamydomonas reinhardii. Cell 36: 925–931 (1984).

    Google Scholar 

  21. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    Google Scholar 

  22. Stinchcomb DT, Mann C, Selker E, Davis RW: DNA sequences that allow the replication and segregation of yeast chromosomes. In: Ray DS (ed) The Initiation of DNA Replication, pp. 473–488. Academic Press, New York (1981).

    Google Scholar 

  23. Stinchcomb DT, Struhl K, Davis RW: Isolation and characterization of a yeast chromosomal replicator. Nature 282: 39–43 (1979).

    Google Scholar 

  24. Struhl K, Stinchcomb DT, Scherer S, Davis RW: High frequency transformation of yeast: Autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci USA 76: 1035–1039 (1979).

    Google Scholar 

  25. Thoma F, Bergmann LW, Simpson RT: Nuclease digestion of circular TRPARS1 chromatin reveals positioned nucleosomes separated by nuclease-sensitive regions. J Mol Biol 177: 715–733 (1984).

    Google Scholar 

  26. Uchimiya H, Ohtani T, Ohgawara T, Harada H, Sugita M, Sugiura M: Molecular cloning of tobacco chromosomal and chloroplast DNA segments capable of replication in yeast. Mol Gen Genet 191: 1–4 (1983).

    Google Scholar 

  27. Vallet J-M, Rahire M, Rochaix J-D: Localization and sequence analysis of chloroplast DNA sequence of Chlamydomonas reinhardii that promote autonomous replication in yeast. EMBO J 3: 415–421 (1984).

    Google Scholar 

  28. Van't Hof J, Bjerknes CA: 18 μm replication units of chromosomal DNA fibers of differented cells of pea (Pisum sativum). Chromosoma 63: 287–294 (1977).

    Google Scholar 

  29. Wang AJ, Quigley GJ, Koplak FJ, Crawford HL, vanBoom JH, vanderMarel G, Rich J: Molecular structure of a lefthanded double helical DNA fragment at atomic resolution. Nature 282: 680–686 (1979).

    Google Scholar 

  30. Zabel P, Meyer D, van deStolpe O, van derZaal B, Ramanna MS, Koornneef M, Krens F, Hille J: Towards the constructiuon of artificial chromosomes for tomato. In: vanVloten-Doting L, Groot GSP, Hall TC (eds) Molecular Form and 0 Function of the Plant Genome, pp. 609–624. Plenum Publishing Corp., New York (1985).

    Google Scholar 

  31. deZamaroczy M, Marotta R, Faugeron-Fonty G, Goursot R, Mangin M, Baldacci G, Bernardi G: The origins of replication of the yeast mitochondrial genome and the phenomenon of suppressivity. Nature 292: 75–78 (1981).

    Google Scholar 

Download references

Author information

Author notes

  1. Roberta E. Berlani

    Present address: Department of Biology, Santa Clara University, 95053, Santa Clara, CA, USA

Authors and Affiliations

  1. Department of Biological Sciences, Stanford University, 94305, Stanford, CA, USA

    Roberta E. Berlani & Virginia Walbot

  2. Department of Biochemistry, Stanford University, 94305, Stanford, CA, USA

    Ronald W. Davis

Authors
  1. Roberta E. Berlani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Virginia Walbot
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ronald W. Davis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Berlani, R.E., Walbot, V. & Davis, R.W. Sequence analysis of three fragments of maize nuclear DNA which replicate autonomously in yeast. Plant Mol Biol 11, 173–182 (1988). https://doi.org/10.1007/BF00015669

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00015669

Key words

Search

Navigation