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Genomic organization of two families of highly repeated nuclear DNA sequences of maize selected for autonomous replicating activity in yeast

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Abstract

Maize nuclear DNA sequences capable of promoting the autonomous replication of plasmids in yeast were isolated by ligating Eco RI-digested fragments into yeast vectors unable to replicate autonomously. Three such autonomously replicating sequences (ARS), representing two families of highly repeated sequences within the maize genome, were isolated and characterized. Each repetitive family shows hybridization patterns on a Southern blot characteristic of a dispersed sequence. Unlike most repetitive sequences in maize, both ARS families have a constant copy number and characteristic genomic hybridization pattern in the inbred lines examined. Larger genome clones with sequence homology to the ARS-containing elements were selected from a lambda library of maize genomic DNA. There was typically only one copy of an ARS-homologous sequence on each 12–15 kb genomic fragment.

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Author notes

  1. Roberta E. Berlani

    Present address: Department of Biology, Santa Clara University, 95053, Santa Clara, CA, USA

Authors and Affiliations

  1. Department of Biochemistry, Stanford University, 94305, Stanford, CA, USA

    Ronald W. Davis

  2. Department of Biological Sciences, Stanford University, 94305, Stanford, CA, USA

    Roberta E. Berlani & Virgina Walbot

Authors
  1. Roberta E. Berlani
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  2. Ronald W. Davis
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  3. Virgina Walbot
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Berlani, R.E., Davis, R.W. & Walbot, V. Genomic organization of two families of highly repeated nuclear DNA sequences of maize selected for autonomous replicating activity in yeast. Plant Mol Biol 11, 161–172 (1988). https://doi.org/10.1007/BF00015668

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  • DOI: https://doi.org/10.1007/BF00015668

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