Abstract
We describe the isolation of the chromosomal gene for pathogenesis-related protein 1a from Nicotiana tabacum. A 2 kb fragment containing the PR-1a gene as well as 5′ and 3′ flanking DNA has been sequenced and the transcriptional start site has been determined by primer extension and S1 nuclease mapping. 80% of the protein sequence from purified PR-1a and 20% of the sequence of purified PR-1b has also been determined and used to verify the nomenclature established for the PR-1 cDNAs.
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Payne, G., Parks, T.D., Burkhart, W. et al. Isolation of the genomic clone for pathogenesis-related protein 1a from Nicotiana tabacum cv. Xanthi-nc. Plant Mol Biol 11, 89–94 (1988). https://doi.org/10.1007/BF00015662
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DOI: https://doi.org/10.1007/BF00015662