Skip to main content
SpringerLink
Log in

Intracellular localization of CA1P and CA1P phosphatase activity in leaves of Phaseolus vulgaris L.

  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

CA1P and CA1P phosphatase occur in the chloroplasts of leaf mesophyll cells of many species. However, whether either may occur exclusively in the chloroplast has not yet been established. To examine their intracellular distribution, mature, dark-or light-treated leaves of Phaseolus vulgaris were frozen, lyophilized and then centrifuged in density gradients of heptane and tetrachloroethylene. After gradient fractionation, both CA1P and CA1P phosphatase activity co-segregated with chloroplast material. Distribution analyses using sub-cellular compartment markers indicated that both CA1P and CA1P phosphatase do occur exclusively in leaf chloroplasts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Bicine:

N,N-bis[2-hydroxyethyl]glycine

CA1P:

2-carboxyarabinitol 1-phosphate

CABP:

2-carboxyarabinitol 1,5-bisphosphate

Chl:

chlorophyll

DTT:

dithiothreitol

EDTA:

(ethylenediamine)tetraacetic acid

PEP:

phosphoenolpyruvate

Tris:

tris(hydroxymethyl)aminomethane

References

  • Andralojc PJ, Dawson GW, Parry MAJ and Keys AJ (1994) Incorporation of carbon from photosynthetic products into 2-carboxyarabinitol-1-phosphate and 2-carboxyarabinitol. Biochem J 304: 781–786

    PubMed  Google Scholar 

  • Berry JA, Lorimer GH, Pierce J, Seemann JR, Meek J and Freas S (1987) Isolation, identification, and synthesis of 2-carboxyarabinitol 1-phosphate, a diurnal regulator of ribulose-bisphosphate carboxylase activity. Proc Natl Acad Sci USA 84: 734–738

    Google Scholar 

  • Gardestrom P and Wigge B (1988) Influence of photorespiration on ATP/ADP ratios in the chloroplasts, mitochondria, and cytosol, studied by rapid fractionation of barley (Hordeum vulgare) protoplasts. Plant Physiol 88: 69–76

    Google Scholar 

  • Gerhardt R and Heldt HW (1984) Measurement of subcellular metabolite levels in leaves by fractionation of freeze-stopped material in nonaqueous media. Plant Physiol 75: 542–547

    Google Scholar 

  • Gutteridge S, Parry MAJ, Burton S, Keys AJ, Mudd A, Feeney J, Servaites JC and Pierce J (1986) A nocturnal inhibitor of carboxylation in leaves. Nature 324: 274–276

    Google Scholar 

  • Gutteridge S, Reddy GS and Lorimer G (1989) The synthesis and purification of 2′-carboxy-d-arabinitol-1-phosphate, a natural inhibitor of ribulose bisphosphate carboxylase investigated by 31P NMR. Biochem J 260: 711–716

    PubMed  Google Scholar 

  • Holbrook G, Bowes G and Salvucci ME (1989) Degradation of 2-carboxyarabinitol 1-phosphate by a specific chloroplast phosphatase. Plant Physiol 90: 673–678

    Google Scholar 

  • Holbrook GP, Turner JA and Polans NO (1992) Dark inhibition of ribulose-1,5-bisphosphate carboxylase/oxygenase in legumes: a biosynthetic study. Photosynth Res 32: 37–44

    Google Scholar 

  • Kobza J, Moore BD and Seemann JR (1990) Carboxyarabinitol-1-P phosphatase of Phaseolus vulgaris. Plant Physiol Suppl 93: 121

    Google Scholar 

  • Kodama T, Fukui K and Kometani K (1986) The initial phosphate burst in ATP hydrolysis by myosin and subfragment-1 as studied by a modified malachite green method for determination of inorganic phosphate. J Biochem 99: 1465–1472

    PubMed  Google Scholar 

  • Moore BD and Seemann JR (1994) Evidence that 2-carboxyarabinitol 1-phosphate binds to ribulose-1,5-bisphosphate carboxylase in vivo. Plant Physiol 105: 731–737

    PubMed  Google Scholar 

  • Moore BD, Kobza J and Seemann JR (1991) Measurement of 2-carboxyarabinitol 1-phosphate in plant leaves by isotope dilution. Plant Physiol 96: 208–213

    Google Scholar 

  • Moore BD, Sharkey TD, Kobza J and Seemann JR (1992) Identification and levels of 2′-carboxyarabinitol in leaves. Plant Physiol 99: 1546–1550

    Google Scholar 

  • Moore BD, Kiirats O, Charlet T and Seemann JR (1995) Levels of 2-carboxyarabinitol 1-phosphate and related photosynthetic components during leaf development in Phaseolus vulgaris. Plant Cell Physiol, in press

  • Portis AR Jr (1992) Regulation of ribulose 1,5-bisphosphate carboxylase/oxygenase activity. Annu Rev Plant Physiol Mol Biol 43: 415–437

    Article  Google Scholar 

  • Rocha V and Ting IP (1970) Preparation of cellular plant organelles from spinach leaves. Arch Biochem Biophys 140: 398–407

    PubMed  Google Scholar 

  • Salvucci ME and Anderson JC (1987) Factors affecting the activation state and the level of total activity of ribulose bisphosphate carboxylase in tobacco protoplasts. Plant Physiol 85: 66–71

    Google Scholar 

  • Salvucci ME and Holbrook GP (1989) Purification and properties of 2-carboxy-d-arabinitol 1-phosphatase. Plant Physiol 90: 679–685

    Google Scholar 

  • Salvucci ME, Holbrook GP, Anderson JC and Bowes G (1988) NADPH-dependent metabolism of the ribulose bisphosphate carboxylase/oxygenase inhibitor 2-carboxyarabinitol 1-phosphate by a chloroplast protein. FEBS Lett 231: 197–201

    Article  Google Scholar 

  • Sage RF, Reid CD, Moore Bd and Seemann JR (1993) Longterm kinetics of the light-dependent regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity in plants with and without 2-carboxyarabinitol 1-phosphate. Planta 191: 222–230

    Article  Google Scholar 

  • Santarius KA and Heber U (1965) Changes in the intracellular level of ATP, ADP, AMP and Pi and regulatory function of the adenylate system in leaf cells during photosynthesis. Biochim Biophys Acta 102: 39–54

    PubMed  Google Scholar 

  • Seemann JR, Berry JA, Freas SM and Krump MA (1985) Regulation of ribulose bisphosphate carboxylase activity in vivo by a light-modulated inhibitor of catalysis. Proc Natl Acad Sci USA 82: 8024–8028

    Google Scholar 

  • Seemann JR, Kobza J and Moore BD (1990) Metabolism of 2-carboxyarabinitol 1-phosphate and regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase during the night. Photosyn Res 23: 119–130

    Google Scholar 

  • Servaites JC (1985) Binding of a phosphorylated inhibitor to ribulose bisphosphate carboxylase/oxygenase during the night. Plant Physiol 78: 839–843

    Google Scholar 

  • Servaites JC (1992) Inhibition of ribulose 1,5-bisphosphate carboxylase/oxygenase by 2-carboxyarabinitol-1-phosphate. Plant Physiol 92: 867–870

    Google Scholar 

  • Sharkey TD and PJ Vanderveer (1989) Stromal phosphate concentration is low during feedback limited photosynthesis. Plant Physiol 91: 679–684

    Google Scholar 

  • Spalding MH, Schmitt MR, Ku SB and Edwards GE (1979) Intracellular localization of some key enzymes of Crassulacean acid metabolism in Sedum praealtum. Plant Physiol 63: 738–743

    Google Scholar 

  • Stitt M, Lilley RMcC, Gerhardt R and Heldt HW (1989) Metabolite levels in specific cells and subcellular compartments of plant leaves. Meth Enzymol 174: 518–552

    Google Scholar 

  • Wintermans JFGM and De Mots A (1965) Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol. Biochim Biophys Acta 109: 448–453

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Nevada, 89557, Reno, NV, USA

    Brandon D. Moore & Jeffrey R. Seemann

  2. Department of Botany, University of Wisconsin, 53706, Madison, WI, USA

    Thomas D. Sharkey

Authors
  1. Brandon D. Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas D. Sharkey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jeffrey R. Seemann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moore, B.D., Sharkey, T.D. & Seemann, J.R. Intracellular localization of CA1P and CA1P phosphatase activity in leaves of Phaseolus vulgaris L.. Photosynth Res 45, 219–224 (1995). https://doi.org/10.1007/BF00015562

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00015562

Key words

Search

Navigation