Skip to main content
SpringerLink
Log in

Mutations responsible for high light sensitivity in an atrazine-resistant mutant of Synechcystis 6714

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

The primary target of photoinhibition is the photosystem II reaction center. The process involves a reversible damage, followed by an irreversible inhibition of photosystem II activity. During cell exposition to high light intensity, the D1 protein is specially degraded. An atrazine-resistant mutant of Synechocystis 6714, AzV, reaches the irreversible step of photoinhibition faster than wild-type cells. Two point mutations present in the psbA gene of AzV (coding for D1) lead to the modification of Phe 211 to Ser and Ala 251 to Val in D1. Transformation of wild-type cells with the AzV psbA gene shows that these two mutations are sufficient to induce a faster photodamage of PSII. Other DCMU-and/or atrazine-resistant mutants do not differ from the wild type when photoinhibited. We conclude that the QB pocket is involved in PSII photodamage and we propose that the mutation of Ala 251 might be related to a lower rate of proteolysis of the D1 protein than in the wild type.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

PSII:

photosystem II

RCII:

reaction center II

References

  1. Astier C, Boussac A, Etienne AL: Evidence for different binding sites on the 33 kDa protein for DCMU, atrazine and QB. FEBS Lett 167: 321–326 (1984).

    Article  Google Scholar 

  2. Astier C, Meyer I, Vernotte C, Etienne AL: Photosystem II electron transfer in highly herbicide resistant mutants of Synechocystis 6714. FEBS Lett 207: 234–238 (1986).

    Article  Google Scholar 

  3. Barber J: Photosynthetic reaction centres: a common link. Trends Biochem Sci 12: 321–326 (1987).

    Article  Google Scholar 

  4. Cleland RE, Critchley C: Studies on the mechanism of photoinhibition in higher plants. Inactivation by high light of photosystem II reaction center function in isolated spinach thylakoids and O2 evolving particles. Photobiochem Photobiophys 10: 83–92 (1985).

    Google Scholar 

  5. Cleland RE, Melis A, Neale PJ: Mechanism of photoinhibition: photochemical reaction center inactivation in System II of chloroplasts. Photosynthesis Res 9: 79–88 (1986).

    Google Scholar 

  6. Cleland RE: Molecular events of photoinhibitory inactivation in the reaction center of photosystem II. Aust J Plant Physiol 15: 135–150 (1988).

    Google Scholar 

  7. Critchley C: The molecular mechanism of photoinhibition-facts and fiction. Aust J Plant Physiol 15: 27–41 (1988).

    Google Scholar 

  8. Curtis SB, Haselkorn R: Isolation, sequence and expression of two members of the 32 Kd thylakoid membrane protein gene family from the cyanobacterium Anabaena 7120. Plant Mol Biol 3: 249–258 (1984).

    Google Scholar 

  9. Demeter S, Neale PJ, Melis A: Photoinhibition: impairment of the primary charge separation between P-680 and pheophytin in photosystem II of chloroplasts. FEBS Lett 214: 370–374 (1987).

    Article  Google Scholar 

  10. Driesel AJ, Speirs J, Bohnert HJ: Spinach chloroplast mRNA for a 32000 dalton polypeptide size and localization on the physical map of the chloroplast DNA. Biochim Biophys Acta 610: 297–310 (1980).

    PubMed  Google Scholar 

  11. Gingrich JC, Buzby JS, Stirewalt VL, Bryant DA: Photosynthesis analysis of two new mutations resulting in herbicide resistance in the cyanobacterium Synechococcus sp PCC 7002. Photosynthesis Res 16: 83–99 (1988).

    Google Scholar 

  12. Golden SS, Haselkorn R: Mutation to herbicide resistance maps within the psbA gene of Anacystis nidulans R2. Science 229: 1104–1107 (1985).

    PubMed  Google Scholar 

  13. Golden SS, Brusslan J, Haselkorn R: Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2. EMBO J 5: 2789–2798 (1986).

    PubMed  Google Scholar 

  14. Greenberg BM, Gaba V, Mattoo AK, Edelman M: Identification of a primary in vivo degradation product of the rapidly turning-over 32Kd protein of photosystem II. EMBO J 6: 2865–2869 (1987).

    PubMed  Google Scholar 

  15. Greer DH, Berry JA, Björkman O: Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature and requirement for chloroplast-protein synthesis during recovery. Planta 168: 253–260 (1986).

    Google Scholar 

  16. Grigorieva GR, Shestakov SV: Transformation, in the cyanobacterium Synechocystis 6803. FEMS Microbiol Lett 13: 367–370 (1982).

    Article  Google Scholar 

  17. Jansson C, Debus RJ, Osiewacz HD, Gurevitz M, McIntosch L: Construction of an obligate photoheterotrophic mutant of the cyanobacterium Synechocystis 6803. Plant Physiol 85: 1021–1025 (1987).

    Google Scholar 

  18. Johanningmeier U, Bodner U, Wildner GF: A new mutation in the gene coding for the herbicide-binding protein in Chlamydomonas. FEBS Lett 211: 221–224 (1987).

    Article  Google Scholar 

  19. Kaiser K, Murray NE: In DNA cloning: a practical approach, Vol 1: 1–47, IRL Press, Oxford (1985).

    Google Scholar 

  20. Kirilovsky D, Vernotte C, Astier C, Etienne AL: Reversible and irreversible photoinhibition in herbicide-resistant mutants of Synechocystis 6714. Biochim Biophys Acta 933: 124–131 (1988).

    Google Scholar 

  21. Kyle DJ, Ohad I, Arntzen CJ: Membrane protein damage and repair I. Selective loss of quinone protein function in chloroplast membranes. Proc Natl Acad Sci USA 81: 4070–4074 (1984).

    Google Scholar 

  22. Kyle DJ: The biochemical basis for photoinhibition of photosystem II. In: Kyle DJ, Osmond CB, Arntzen CJ (eds) Photoinhibition, Chapter 9, pp. 197–226. Elsevier, Amsterdam (1987).

    Google Scholar 

  23. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature New Biol 277: 680–685 (1970).

    Google Scholar 

  24. Lönneborg A, Kalla SR, Samuelsson G, Gustafsson P, Oquist G: Light-regulated expression of the psbA transcript in the cyanobacterium Anacystis nidulans. FEBS Lett 240: 110–114 (1988).

    Article  Google Scholar 

  25. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, Ny (1982).

    Google Scholar 

  26. Michel H and Deisenhofer J: Relevance of the photosynthetic reaction center from purple bacteria to the structure of photosystem II. Biochemistry 27: 1–7 (1988).

    Google Scholar 

  27. Mulligan B, Schulter N, Chen L, Bogorad L: Nucleotide sequence of a multiple copy gene for the B protein of photosystem II of a cyanobacterium. Proc Natl Acad Sci USA 81: 2693–2697 (1984).

    Google Scholar 

  28. Nanba O and Satoh K: Isolation of a photosystem II reaction center consisting of a D1 and D2 polypeptides and cytochrome b559. Proc Natl Acad Sci USA 84: 109–112 (1987).

    Google Scholar 

  29. Ohad I, Kyle DJ, Arntzen CJ: Membrane protein damage and repair removal and replacement of inactivated 32 Kd polypeptides in chloroplast membranes. J Cell Biol 99: 481–485 (1985).

    Article  Google Scholar 

  30. Ohad I, Koike H, Shochat S, Inoue Y: Changes in the properties of reaction center II during the initial stages of photoinhibition as revealed by thermoluminescence measurements. Biochim Biophys Acta 933: 288–298 (1988).

    Google Scholar 

  31. Powles SB, Björkman O: Photoinhibition of photosynthesis: effect on chlorophyll fluorescence at 77°K in intact leaves and in chloroplast membranes of Nerium oleander. Planta 156: 97–107 (1982).

    Google Scholar 

  32. Reisman S, Michaels M, Ohad I: Lack of recovery from photoinhibition in a temperature-sensitive Chlamydomonas reinhardtii mutant T44 unable to synthesize and/or integrate the QB protein of photosystem II at 37°C. Biochim Biophys Acta 849: 41–50 (1986).

    Google Scholar 

  33. Rogers S, Wells R, Rechsteiner M: Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. Science 234: 364–368 (1986).

    PubMed  Google Scholar 

  34. Schuster G, Timberg R and Ohad I: Turnover of thylakoid photosystem II proteins during photoinhibition of Chlamydomonas reinhardtii. Eur J Biochem 177: 403–410 (1988).

    PubMed  Google Scholar 

  35. Toneguzzo F, Glyn S, Levi E, Mjolsness S, Hayday A: Use of chemically modified T7 DNA polymerase for manual and automated sequencing of supercoiled DNA. Biotechniques 6: 460–469 (1988).

    PubMed  Google Scholar 

  36. Trebst A: The three-dimensional structure of the herbicide binding niche on the reaction center polypeptides of photosystem II. Z Naturforsch 42c: 742–750 (1987).

    Google Scholar 

  37. Trebst A and Depka B: Antennas and reaction centers of photosynthetic bacteria-Structure, interactions and dynamics. In: Michel ME (ed) Springer Series in Chemical Physics, Vol 42, pp. 216–226, Springer-Verlag, Berlin (1985).

    Google Scholar 

  38. Vernotte C, Etienne AL, Briantais JM: Analysis of the kinetics of the cation-induced increase in photosystem II fluorescence in isolated thylakoids. Biochim Biophys Acta 545: 519–527 (1982).

    Google Scholar 

  39. Zurawski G, Bonhert HJ, Whitfield PR, Bottomley W: Nucleotide sequence of the gene for the M4 32000 thylakoid membrane protein from Spinacia oleracea and Nicotiana debneyi predicts a totally conserved primary translation product of Mr 38950. Proc Natl Acad Sci USA 79: 7699–7703 (1982).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. UPR 407, CNRS, 91198, Gif sur Yvette, Cedex, France

    Diana L. Kirilovsky, Ghada Ajlani, Martine Picaud & Anne-Lise Etienne

Authors
  1. Diana L. Kirilovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ghada Ajlani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Martine Picaud
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anne-Lise Etienne
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kirilovsky, D.L., Ajlani, G., Picaud, M. et al. Mutations responsible for high light sensitivity in an atrazine-resistant mutant of Synechcystis 6714. Plant Mol Biol 13, 355–363 (1989). https://doi.org/10.1007/BF00015547

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00015547

Key words

Search

Navigation