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Polyamines in the photosynthetic apparatus

Photosystem II highly resolved subcomplexes are enriched in spermine

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Abstract

The three main polyamines putrescine (Put), spermidine (Spd) and spermine (Spm) were characterized by HPLC in intact spinach leaf cells, intact chloroplasts, thylakoid membranes, Photosystem II membranes, the light-harvesting complex and the PS II complex. All contain the three polyamines in various ratios; the HPLC polyamine profiles of highly resolved PS II species (a Photosystem II core and the rection center) suggest an enrichment in the polyamine Spm.

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Abbreviations

Chl:

chlorophyll

HPLC:

high performance liquid chromatography

LHC:

light-harvesting complex

PS II:

Photosystem II

PS II-RC:

Photosystem II reaction center

Put:

putrescine

Spd:

spermidine

Spm:

spermine

10%S-core:

D1-D2-Cyt b559-47 kD-43 kD complex

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Authors and Affiliations

  1. Department of Biology, University of Crete, Heraklion, Crete, Greece

    Kiriakos Kotzabasis & Kalliopi A. Roubelakis-Angelakis

  2. Department of Chemistry, University of Crete, Heraklion, Crete, Greece

    Constantina Fotinou & Demetrios Ghanotakis

  3. Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Heraklion, Crete, Greece

    Constantina Fotinou & Demetrios Ghanotakis

Authors
  1. Kiriakos Kotzabasis
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  2. Constantina Fotinou
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  3. Kalliopi A. Roubelakis-Angelakis
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  4. Demetrios Ghanotakis
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Kotzabasis, K., Fotinou, C., Roubelakis-Angelakis, K.A. et al. Polyamines in the photosynthetic apparatus. Photosynth Res 38, 83–88 (1993). https://doi.org/10.1007/BF00015064

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  • DOI: https://doi.org/10.1007/BF00015064

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