Abstract
Complementary DNA sequences were isolated from a library of cloned Arabidopsis leaf mRNA sequences in λgt10 that encoded a 21.7 kDa polypeptide (CaBP-22), which shared 66% amino acid sequence identity with Arabidopsis calmodulin. The putative Ca2+-binding domains of CaBP-22 and calmodulin, however, were more conserved and shared 79% sequence identity. Ca2+ binding by CaBP-22, which was inferred from its amino acid sequence similarity with calmodulin, was demonstrated indirectly by Ca2+-induced mobility shifting of in vitro translated CaBP-22 during SDS-polyacrylamide gel electrophoresis. CaBP-22 is encoded by a ca. 0.9 kb mRNA that was detected by northern blotting of leaf poly(A)+ RNA; this mRNA was slightly larger than the 809 bp CaBP-22 cDNA insert, indicating that the deduced amino acid sequence of CaBP-22 is near full-length. CaBP-22 mRNA was detected in RNA fractions isolated from leaves of both soil-grown and hydroponically grown Arabidopsis, but below the limits of detection in RNA isolated from roots, and developing siliques. Thus, CaBP-22 represents a new member of the EF-hand family of Ca2+-binding proteins with no known animal homologue and may participate in transducing Ca2+ signals to a specific subset of response elements.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Babu YS, Bugg CE, Cook WJ: Structure of calmodulin refined at the 2.2A resolution. J Mol Biol 204: 191–204 (1988).
Beltz GA, Jacobs KA, Eickbush TH, Cherbas PT, Kafatos F: Isolation of multigene families and determination of homologies by filter hybridization methods. Meth Enzymol 100: 266–285 (1983).
Braam J, Davis Rw: Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis. Cell 60: 357–364 (1990).
Burgess WH, Jemiolo DK, Kretsinger RH: Interaction of calcium and calmodulin in the presence of sodium dodecyl sulfate. Biochim Biophys Acta 623: 257–270 (1980).
Erickson AH, Blobel G: Cell-free translation of messenger RNA in a wheat germ system: Meth Enzymol 96: 38–50 (1983).
Felle H: Auxin causes oscillations of cytosolic free calcium and pH in Zea mays coleoptiles. Planta 174: 495–499 (1988).
Gawienowski MC, Szymanski D, Perera IY, Zielinski RE: Calmodulin isoforms in Arabidopsis encoded by multiple divergent mRNAs. Plant Mol Biol, in press.
Gehring CA, Williams DA, Cody SH, Parish RW: Phototropism and geotropism in maize coleoptiles are spatially correlated with increases in cytosolic free calcium. Nature 345: 528–530 (1990).
Harper J, Sussman MR, Schaller GE, Putnam-Evans C, Charbonneau H, Harmon AC: A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science 252: 951–954 (1991).
Herzberg O, James MNG: Structure of the calcium regulatory muscle protein troponin-C at 2.8 A resolution. Nature 313: 653–659 (1985).
Hodgson CP, Fisk RZ: Hybridization probe size control: optimized ‘oligolabelling’. Nucl Acids Res 15: 6295 (1987).
Ikura M, Clore GM, Gronenborn AM, Zhu G, Klee CB, Bax A: Solution structure of a calmodulin-target peptide complex by multidimensional NMR. Science 256: 632–638 (1992).
Jablonsky PP, Grolig F, Perkin JL, Williamson RE: Properties of monoclonal antibodies to plant calmodulin. Plant Sci 76: 175–184 (1991).
Johannes E, Brosnan JM, Sanders D: Calcium channels and signal transduction in plant cells. BioEssays 13: 331–336 (1991).
Ling V, Perera IY, Zielinski RE: Primary structures of Arabidopsis calmodulin isoforms deduced from the sequences of cDNA clones. Plant Physiol 96: 1196–1202 (1991).
Ling V, Zielinski RE: Cloning of cDNA sequences encoding the calcium-binding protein calmodulin, from barley (Hordeum vulgare L.). Plant Physiol 90: 714–719 (1989).
McAinsh MR, Brownlee C, Hetherington AM: Abscisic acid-induced elevation of guard cell cytosolic Ca2+ precedes stomatal closure. Nature 343: 186–188 (1990).
Moncrief ND, Kretsinger RH, Goodman M: Evolution of EF-hand calcium-modulated proteins. I. Relationships based on amino acid sequences. J Mol Evol 30: 522–562 (1990).
O'Neil KT, DeGrado WF: How calmodulin binds its targets: sequence independent recognition of amphiphilic α-helices. Trends Biochem Sci 15: 59–64 (1990).
Perera IY, Zielinski RE: Structure and expression of the Arabidopsis CaM-3 calmodulin gene. Plant Mol Biol 19: 649–664 (1992).
Persechini A, Kretsinger RH: The central helix of calmodulin functions as a flexible tether. J Biol Chem 263: 12175–12178 (1988).
Persechini A, Moncrief ND, Kretsinger RH: The EF-hand family of calcium-modulated proteins. Trends Neuro Sci 11: 462–467 (1989).
Roberts DM, Lukas TJ, Watterson DM: Structure, function and mechanism of action of calmodulin. CRC Crit Rev Plant Sci 4: 311–339 (1986).
Suen K-L, Choi JH: Isolation and sequence analysis of a cDNA clone for a carrot calcium-dependent protein kinase: homology to calcium/calmodulin-dependent protein kinases and to calmodulin. Plant Mol Biol 17: 581–590 (1991).
Trewavas A, Gilroy S: Signal transduction in plant cells. Trends Genet 7: 356–361 (1991).
Weber PC, Lukas TJ, Craig TA, Wilson E, King MM, Kwiatkowski AP, Watterson DM: Computational and site-specific mutagenesis analyses of the asymmetric charge distribution on calmodulin. Proteins: Struct Funct Genet 6: 70–85 (1989).
Zielinski RE: Calmodulin mRNA in barley (Hordeum vulgare L.): apparent regulation by cell proliferation and light. Plant Physiol 84: 937–943.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ling, V., Zielinski, R.E. Isolation of an Arabidopsis cDNA sequence encoding a 22 kDa calcium-binding protein (CaBP-22) related to calmodulin. Plant Mol Biol 22, 207–214 (1993). https://doi.org/10.1007/BF00014929
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00014929