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Chlorophyll-protein and polypeptide composition of Mn-deficient sugar beet thylakoids

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Abstract

The chlorophyll-protein and polypeptide composition of manganese deficient and control sugar beet thylakoids was examined using three different detergent-electrophoresis systems. On a per chlorophyll basis, manganese deficiency reduced the amounts of CPa complex (separated by sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis), and CP 47 and CP 43 complexes (separated by octylglucoside/SDS-polyacrylamide gel electrophoresis) without decreasing the amounts of light harvesting complexes. Lithium dodecylsulfate/Triton X-100 polyacrylamide gel electrophoresis showed that manganese deficiency decreased several thylakoid polypeptides, including a chlorophyll b containing 30 kilodalton chlorophyll-protein complex, but did not decrease the amounts of 28 and 29 kilodalton light-harvesting chlorophyll b-containing polypeptides.

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Author notes

  1. Javier Abadia

    Present address: Estacion Experimental de Aula Dei, (C.S.I.C.), Ap. 202, Zaragoza, Spain

Authors and Affiliations

  1. Department of Plant and Soil Biology, University of California, 94720, Berkeley, CA, USA

    Javier Abadia, John N. Nishio & Norman Terry

Authors
  1. Javier Abadia
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  2. John N. Nishio
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  3. Norman Terry
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Abadia, J., Nishio, J.N. & Terry, N. Chlorophyll-protein and polypeptide composition of Mn-deficient sugar beet thylakoids. Photosynth Res 7, 237–245 (1986). https://doi.org/10.1007/BF00014677

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  • DOI: https://doi.org/10.1007/BF00014677

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