Abstract
The complete amino acid sequence of a cytoplasmic ribosomal protein S13 of maize was deduced from the cDNA isolated from a maize cDNA library. The encoded protein is 151 amino acids long and shows a homology of 73% with the corresponding protein S13 of rat. Southern blots analysis shows that the maize protein S13 is encoded by a small multigene family conserved in plant species closely related to maize. The S13 RNAs accumulate preferentially in proliferating tissues and cells and their transcription occurs in parallel to the DNA synthesis.
References
Atanassova R, Chaubet N, Gigot C: A 126 pb fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis. Plant J 2: 291–300 (1992).
Chaubet N, Clément B, Philipps G, Gigot C: Organspecific expression of different histone H3 and H4 gene subfamilies in developing and adult maize. Plant Mol Biol 17: 935–940 (1991).
Gantt JS, Thompson MD: Plant cytosolic ribosomal protein S11 and chloroplast ribosomal protein S17. Their primary structure and evolutionary relationships. J Biol Chem 265: 2763–2767 (1990).
Köhler S, Coraggio I, Becker D, Salamini F: Pattern of expression of meristem-specific cDNA clones of barley (Hordeum vulgare L.). Planta 186: 227–235 (1992).
Larkin JC, Hunsperger JP, Culley D, Rubenstein I, Silflow CD: The organization and expression of a maize ribosomal protein gene family. Genes Devel 3: 500–509 (1989).
Lebrun M, Freyssinet G: Nucleotide sequence and characterization of a maize cytoplasmic ribosomal protein S11 cDNA. Plant Mol Biol 17: 265–268 (1991).
Mager WH: Control of ribosomal protein gene expression. Biochim Biophys Acta 949: 1–15 (1988).
Marty I, Meyer Y: cDNA nucleotide sequence and expression of a tobacco cytoplasmic ribosomal protein L2 gene. Nucl Acids Res 20: 1517–1522 (1992).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Suzuki K, Olvera J, Wool IG: The primary structure of rat ribosomal protein S13. Biochem Biophys Res Comm 171: 519–524 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Joanin, P., Gigot, C. & Philipps, G. cDNA nucleotide sequence and expression of a maize cytoplasmic ribosomal protein S13 gene. Plant Mol Biol 21, 701–704 (1993). https://doi.org/10.1007/BF00014553
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00014553