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Fractionation of sediment phosphate with chelating compounds: a simplification, and comparison with other methods

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Abstract

The NTA/EDTA fractionation of sediment-bound, inorganic phosphate was improved and made more effective. The iron-bound phosphate is now extracted with Ca-EDTA plus dithionite and followed by an extraction of calcium-bound phosphate by Nat-EDTA at pH = 4.5. A comparison is made with two other sequential extraction procedures: the SEDEX and the Hieltjes & Lijklema extractions. The EDTA extractions have an advantage over the other two methods.

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Abbreviations

o-P =:

ortho phosphate

ASOP=:

Acid Soluble Organic Phosphate

TRIS-buffer=:

tris (hydroximethyl)-amino methane

NTA=:

Nitrilo triacetic acid (or its salts)

M =:

mol dm 3

EDTA=:

Ethylene dinitrilo tetraacetic acid(or its salts)

Fe(OOH)≈P =:

iron-bound phosphate

CaCO3 ≈P =:

calcium-bound phosphate

NaOH-Pextr =:

phosphate extracted with NaOH (etc.)

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Author notes

  1. H. L. Golterman

    Present address: Mas dou Bòchi, Ch. de Tintarlot, F-13200, Arles, France

Authors and Affiliations

  1. Association ‘Leiden-Camargue’, Station Biologique de la Tour du Valat, le Sambuc, F-13200, Arles, France

    H. L. Golterman

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  1. H. L. Golterman
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Golterman, H.L. Fractionation of sediment phosphate with chelating compounds: a simplification, and comparison with other methods. Hydrobiologia 335, 87–95 (1996). https://doi.org/10.1007/BF00013687

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