Abstract
A broad-host-range plasmid (pEA2-21) containing a Bradyrhizobium sp (F-4) nod DABC-lacZ translation fusion was constructed and used to monitor nod gene expression in response to pigeonpea root exudate. Two nod-inducing compounds were isolated and identified. Spectral analysis using ultraviolet absorption, infrared spectra, proton nuclear magnetic resonance, and mass spectrometry showed that the two inducers were 5,4′-dihydroxy-6-(3‴-methyl-2‴-butenyl)-2‴, 2″-dimethyl pyrano-[5″, 6″:7, 8]-flavanone (cajaflavanone) and 2′,4′,5′-trihydroxy-5-isopentenyl-6, 7-dimethylchromene iso-flavanone (cajanone). When pEA2-21 was introduced into Rhizobium trifolii and R. meliloti cajanone and cajaflavanone did not induce nod gene indicating that specificity of induction appears to be influenced by the host-strain genome.
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Dahiya, J.S. Cajaflavanone and cajanone released from Cajanus cajan (L. Millsp.) roots induce nod genes of Bradyrhizobium sp.. Plant Soil 134, 297–304 (1991). https://doi.org/10.1007/BF00012049
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DOI: https://doi.org/10.1007/BF00012049