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Assay for soil urease activity

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Summary

A procedure is described that allows assay of soil urease activity. The method uses a phosphate buffer (pH 8.8) and a urea substrate concentration of 0.007 M. Incubation for 4 h at 37°C is recommended and urease activity is estimated by determining the amount of ammonium produced by urea hydrolysis in soil. The method is precise, and compares favourably with other procedures. re]19750710

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Authors and Affiliations

  1. Soil Science Section, Faculty of Agriculture and Forestry, University of Melbourne, 3052, Parkville, Victoria, Australia

    P. B. May & L. A. Douglas

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  1. P. B. May
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  2. L. A. Douglas
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May, P.B., Douglas, L.A. Assay for soil urease activity. Plant Soil 45, 301–305 (1976). https://doi.org/10.1007/BF00011156

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  • DOI: https://doi.org/10.1007/BF00011156

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