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Influence of adenine, isopentyl alcohol and Azotobacter chroococcum on the vegetative growth of Zea mays

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Abstract

Glasshouse experiments were conducted to evaluate the influence of pretested cytokinin precursors, adenine (ADE) and isopentyl alcohol (IA), and a cytokinin producing bacterium, Azotobacter chroococcum added to soil, on the vegetative growth of maize (Zea mays L.). The combination of 2.0 mg ADE kg-1 soil, 13 mg IA kg-1 soil, plus an inoculum of A. chroococcum was the most effective in enhancing the vegetative growth of maize compared with the application of ADE plus IA, ADE plus A. chroococcum, or ADE, IA or A. chroococcum alone. The dry weight of root and shoot tissues was increased up to 5.57- and 5.01-fold, respectively, in comparison to the controls; however, the root/shoot ratios were similar. The increases in shoot height, internodal distance, stem and leaf width over the controls under the optimum treatment were: 2.07-, 2.81-, 1.46-, and 2.11-fold, respectively. The improvement in plant yield was primarily attributed to A. chroococcum production of cytokinins in the rhizosphere.

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References

  • Arshad M and Frankenberger W TJr 1990a Response of Zea mays and Lycopersicon esculentum to the ethylene precursor, L-methionine and L-ethionine applied to soil. Plant and Soil 122, 219–227.

    CAS  Google Scholar 

  • Arshad M and Frankenberger W TJr 1990b. Microbial production of plant hormones. Plant and Soil 133, 1–8.

    Article  Google Scholar 

  • Arshad M and Frankenberger W TJr 1990c Microbial production of plant growth regulators. In Microbial Technologies. Ed. BMetting. Marcel Dekker, New York (In press).

    Google Scholar 

  • Audus L J 1959 Plant growth substances. pp 405–414. Interscience Publishers, Inc., New York.

    Google Scholar 

  • Barea J M, Navarro E and Montoya E 1976 Production of plant growth regulators by rhizosphere phosphate-solubilizing bacteria. J. Appl. Bacteriol. 40, 129–134.

    PubMed  CAS  Google Scholar 

  • Bennici A and Cionini P G 1979 Cytokinins and in vitro development of Phaseolus coccineus embryos. Planta 147, 27–29.

    Article  CAS  Google Scholar 

  • Brown M E 1976 Role of Azotobacter paspali in association with Paspalum notatum. J. Appl. Bacteriol. 40, 341–348.

    Google Scholar 

  • Cooper R 1959 Bacterial fertilizers in the Soviet Union. Soils Fertil. 22, 327–333.

    Google Scholar 

  • Duncan D B 1955 Multiple range and multiple F-tests. Biometrics 11, 1–42.

    Article  Google Scholar 

  • Frankenberger W TJr and Poth M 1987 Biosynthesis of indole-3-acetic acid by the pine ectomycorrhizal fungus Pisolithus tinctorius. Appl. Environ. Microbiol. 53, 2908–2913.

    PubMed  CAS  Google Scholar 

  • Frankenberger W TJr, Chang A and Arshad M 1990 Response of Raphanus sativus to the auxin precursor, L-tryptophan applied to soil. Plant and Soil 129, 235–241.

    CAS  Google Scholar 

  • Hussain A, Arshad M, Hussain A and Hussain F 1987 Response of maize (Zea mays) to Azotobacter inoculation under fertilized and unfertilized conditions. Biol. Fertil. Soils 4, 73–77.

    Google Scholar 

  • Jagnow G 1987 Inoculation of cereal crops and forage grasses with nitrogen-fixing rhizosphere bacteria: Possible causes of success and failure with regard to yield response — A review. Z. Pflanzenernaehr. Bodenkd. 150, 361–368.

    Google Scholar 

  • Kuiper D and Staal M 1987 The effects of exogenously applied plant growth substances on the physiological plasticity in Plantago major spp. pleisosperma: Responses of growth, shoot to root ratio and respiration. Physiol. Plant. 69, 651–658.

    Article  CAS  Google Scholar 

  • Kuiper D, Kuiper P J C, Lambers H, Schuit J, and Staal M 1989 Cytokinin concentration in relation to mineral nutrition and benzyladenine treatment in Plantago major spp. pleiosperma. Physiol. Plant. 75, 511–517.

    Article  CAS  Google Scholar 

  • Maasz H and Klämbt D 1981 On the biogenesis of cytokinins in roots of Phaseolus vulgaris. Planta 151, 353–358.

    Article  Google Scholar 

  • Macura J 1965 Interrelations between microorganisms and plant in the rhizosphere. In Plant Microbes Relationships. Eds. J Macura and V Vancura. pp 26–33. Prag.

  • Miura G A and Miller C O 1969 6-(γ,γ-Dimethylallylamino)purine as a precursor of zeatin. Plant Physiol. 44, 372–376.

    Article  PubMed  CAS  Google Scholar 

  • Murai N 1980 Cytokinin biosynthesis and its relationship to the presence of plasmids in strains of Corynebacterium fascians. In Metabolism and Molecular Activities of Cytokinins. Eds. JGuern and CPéaud-Lenoël. pp 1–26. Springer-Verlag, Berlin, Heidelberg, New York.

    Google Scholar 

  • Nieto K F and Frankenberger W TJr 1988 Determination of cytokinins by ion suppression-reverse phase high performance liquid chromatography. J. Liquid Chromatogr. 11, 2907–2925.

    CAS  Google Scholar 

  • Nieto K F and Frankenberger W TJr 1989a Biosynthesis of cytokinins produced by Azotobacter chroococcum. Soil Biol. Biochem. 21, 967–972.

    Article  CAS  Google Scholar 

  • Nieto K F and Frankenberger W TJr 1989b Biosynthesis of cytokinins in soil. Soil Sci. Soc. Am. J. 53, 735–740.

    Article  CAS  Google Scholar 

  • Nieto K F and Frankenberger W TJr 1990a Microbial production of cytokinins. In Soil Biochemistry. Eds. J MBollag and GStotzky. Vol. 6, pp 191–248. Marcel Dekker, New York.

    Google Scholar 

  • Nieto K F and Frankenberger W TJr 1990b Influence of adenine, isopentyl alcohol and Azotobacter chroococcum on the growth of Raphanus sativus. Plant and Soil 127, 147–156.

    Article  CAS  Google Scholar 

  • Steel R G D and Torrie J H 1980 Principles and Procedures of Statistics — A Biometrical Approach. 2nd Ed., pp 173–177. McGraw-Hill, New York.

    Google Scholar 

  • Stenlid G 1982 Cytokinins as inhibitors of root growth. Physiol. Plant. 56, 500–506.

    Article  CAS  Google Scholar 

  • Stuchbury T, Palni L M S, Horgan R and Wareing P F 1979 The biosynthesis of cytokinins in crown gall tissue of Vinca rosea. Planta 147, 97–102.

    Article  CAS  Google Scholar 

  • Tien T M, Gaskins M H and Hubbell D H 1979 Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Appl. Environ. Microbiol. 37, 1016–1024.

    PubMed  CAS  Google Scholar 

  • Wareing P F and Phillips D J 1981 Growth and Differentiation in Plants. 3rd Ed., pp 64–66. Pergamon Press, New York.

    Google Scholar 

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Nieto, K.F., Frankenberger, W.T. Influence of adenine, isopentyl alcohol and Azotobacter chroococcum on the vegetative growth of Zea mays . Plant Soil 135, 213–221 (1991). https://doi.org/10.1007/BF00010909

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