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The effect of root temperature on the induction of nitrate reductase activities and nitrogen uptake rates in arctic plant species

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Abstract

We investigated whether six arctic plant species have the potential to induce nitrate reductase (NR) activity when exposed to NO3 --nitrogen under controlled environment conditions, using an in vivo assay that uses the rate of NO2 --accumulation to estimate potential NR activity. We also assessed the effect of low root temperatures on NR activity, growth and nitrogen uptake (using 15N applications) in two of the selected species. Five of the six species (Cerastium alpinum, Dryas intergrifolia, Oxyria digyna, Saxifraga cernua and Salix arctica) were capable of inducing NR activity when exposed to solutions containing 0.5 mM NO3 - at 20°C for 10 days. Although in vivo NR activity was not induced in Saxifraga oppositifolia under controlled conditions, we conclude that it was capable of growing successfully on NO3 -, due to the presence of moderate rates of NR activity observed in both NH4 +-grown and NO3 --treated plants. Exposure of O. digyna and D. integrifolia to 3°C root temperatures for two weeks, with the shoots kept at 20°C, resulted in root and leaf NR activity rates of NO3 --treated plants being reduced to rates exhibited by NH4 +-grown plants. Although these decreases in NR in both species appeared to be due to limitations in NO3 --uptake and growth rate (rather than direct low-temperature inhibition of NR synthesis per se), direct low-temperature inhibition of root NR synthesis could not be ruled out. In contrast to the temperature insensitivity of NH4 + uptake in D. integrifolia, NO3 --uptake in D. integrifolia was inhibited by low root temperatures. We conclude that the selected arctic species have the genetic potential to utilize NO3 --nitrogen, and that low root temperatures, in conjunction with other environmental limitations, may be responsible for the lack of induction of NR in D. integrifolia and Salix arctica under field conditions.

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Abbreviations

NR:

Nitrate reductase

RGR:

relative growth rate

RWR:

root weight ratio

DMC:

dry matter content

NUR:

nitrogen uptake rate

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Author notes

  1. Owen K. Atkin

    Present address: Department of Plant Ecology and Evolutionary Biology, Utrecht University, Sorbonnelaan 16, P.O. Box 800.84, 3508 TB, Utrecht, The Netherlands

Authors and Affiliations

  1. Department of Botany, Erindale College, University of Toronto, L5L 1C6, Mississauga, Ontario, Canada

    Owen K. Atkin & W. Raymond Cummins

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  1. Owen K. Atkin
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  2. W. Raymond Cummins
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Atkin, O.K., Cummins, W.R. The effect of root temperature on the induction of nitrate reductase activities and nitrogen uptake rates in arctic plant species. Plant Soil 159, 187–197 (1994). https://doi.org/10.1007/BF00009280

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