Synopsis
Numbers of offspring produced by genetically marked male and female rainbow trout,Oncorhynchus mykiss were determined by an electrophoretic analysis of 1713 progeny from crosses produced by premixing gametes from different parents prior to insemination and associated full- and half-sib controls. We examined whether male potency, defined as the ability of males to sire progeny when their semen is pooled with that of other males, is affected by interaction with female gametes, concentration of sperm cells and the timing of semen application. Male potency differed significantly and was not affected by which of two females was involved in either of two sets of experiments. The differential between males decreased significantly when sperm concentrations were equalized. The first male sired over 75% of progeny when semen from four males was added sequentially to ova at 30 s intervals in each of two experiments. The lack of significant deviations of marker genotypes from expected Mendelian segregation in full-sib families suggested that there was no differential survival of progeny with specific marker genotypes. Furthermore, there was no correspondence between the survival of progeny of specific males in control crosses and the number of progeny sired in matings where sperm was premixed prior to insemination. Thus. variation in male reproductive success appeared the result of differential fertilization rates.
Similar content being viewed by others
References cited
Aas, G.H., T. Refstie & B. Gjerde. 1991. Evaluation of milt quality of Atlantic salmon. Aquaculture 95: 125–132.
Allendorf, F.W., N. Mitchell, N. Ryman & G. St»hl. 1977. Isozyme loci in the brown trout (Salmo trutta L.): detection and interpretation from population data. Hereditas 86: 179–190.
Allendorf, F.W. & N. Ryman. 1987. Genetic management of hatchary stocks. pp. 141–159. In: N. Ryman & F. Utter (ed.) Population Genetics and Fishery Management, University of Washington Press, Seattle.
Balon, E.K. 1985. The theory of saltatory ontogeny and life history models revisited. pp. 13–30. In: E.K. Balon.
Blaber, A.P. 1986. Relationship between transmembrane potential and activation of motility in spermatozoa of rainbow trout (Salmo gairdneri). M.Sc. Thesis, University of Guelph. Guelph. 109pp.
Bouck, G.R. & J. Jacobson. 1976. Estimation of salmonid sperm concentration by microhematocrit technique. Trans. Amer. Fish. Soc. 105: 534–535.
Danzmann, R.G. & M.M. Ferguson. 1988. Temperature-dependent genotypic selection and embryonic survival of rainbow trout. Biochem. Genet. 26: 69–81.
Ferguson, M.M., P.E. Ihssen & J.D. Hynes. 1991. Are cultured stocks of brown trout and rainbow trout genetically similar to their source populations? Can. J. Fish. Aquat. Sci. 48: 118–123.
Gharrett, A.J. & S.M. Shirley. 1985. A genetic examination of spawning methodology in a salmon hatchery. Aquaculture 47: 245–256.
Gile, S.R. & M.M. Ferguson. 1990. Crossing methodology and genotypic diversity in a hatchery strain of rainbow trout (Oncorhynchus mykiss). Can. J. Fish. Aquat. Sci. 47: 719–724.
Gilkey, J.C. 1981. Mechanisms of fertilization in fishes. Amer. Zool. 21: 359–375.
Ginsburg, A.S. 1963. Sperm-egg association and its relationship to the activation of the egg in salmonid fishes. J. Embryol. Esp. Morph. 11: 13–33.
Hindar, K., N. Ryman & F. Utter. 1991. Genetic effects of cultured fish on natural fish populations. Can. J. Fish. Aquat. Sci. 48: 945–957.
Laale, H.W. 1980. The perivitelline space and egg envelopes in bony fishes: a review. Copeia 1980: 210–226.
Levanduski, M.J. & J.G. Cloud. 1988. Rainbow trout (Salmo gairdneri) semen: effect of non-motile sperm on fertility. Aquaculture 75: 171–179.
Moccia, R.D. & K.R. Munkittrick. 1987. Relationship between the fertilization of rainbow trout (Salmo gairdneri) eggs and motility of spermatozoa. Theriogeneology 27: 679–688.
Munkittrick, K.R. & R.D. Moceia. 1987. Seasonal changes in the quality of rainbow trout (Salmo gairdneri) semen: effect of delay in stripping on spermatocrit, motility, volume and seminal plasma constituents. Aquaculture 64: 141–156.
Terner, C. 1986. Evaluation of salmonid sperm motility for cryo-reservation. Prog. Fish Cult. 48: 230–232.
Watanabe, T., T. Iwamatsu, T. Ohta, K. Onitake, O.P. Saxena & M. Tadano. 1988. Antigenicity on sperm surface of the rainbow troutSalmo gairdneri. Ind. J. Exp. Biol. 26: 345–351.
Withler, R.E. 1988. Genetic consequences of fertilizing Chinook salmon (Oncorhynchus tshawytscha) eggs with pooled milt. Aquaculture 68: 15–25.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gile, S.R., Ferguson, M.M. Factors affecting male potency in pooled gamete crosses of rainbow trout,Oncorhynchus mykiss . Environ Biol Fish 42, 267–275 (1995). https://doi.org/10.1007/BF00004920
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00004920