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Seasonal changes in the biochemistry of seminal plasma and sperm motility in the ocean pout,Macrozoarces americanus

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Abstract

Sperm motility, pH and osmolality of seminal plasma varied throughout the reproductive season spanning the period from June to September. Initially, sperm motility was low, peaked in July and August and then fell again towards the end of the spawning season. While the pH of seminal plasma increased from pH 7.4 to 7.9 during the period of spermiation, the average seasonal pH (7.78 ± 0.03) remained close to an experimentally determined optimum pH range for ocean pout sperm motility (pH 8–9). Likewise, although the values for seminal plasma osmolality fell during the reproductive season, from 416–339 mmol kg-1, the average osmolality value 356 ± 3 was within the optimum for sperm motility (300–400 mmol kg-1). In comparing fluctuations in sperm motility with the biochemical composition of ocean pout seminal plasma during the spawning season, this analysis showed that increased Mg++ levels were correlated with the summer period of maximum sperm motility. A seasonal decline in Na+ and Cl− ion levels was reflected in lower seminal plasma osmolality values.

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References cited

  • Billard, R. 1986. Spermatogenesis and spermatology of some teleost fish species. Reprod. Nutr. Develop. 26: 877–920.

    Google Scholar 

  • Billard, R. 1990. Artificial insemination in fish.In Marshall's Physiology of Reproduction. Vol. 2, pp. 870–888. Edited by G.E. Lamming. Churchill Livingstone, New York.

    Google Scholar 

  • Billard, R. and Cosson, M.P. 1990. The energetics of fish sperm motility.In Controls of Sperm Motility: Biological and Clinical Aspects. pp. 153–173. Edited by C. Gagnon. CRC Press, Boca Raton.

    Google Scholar 

  • Billard, R. and Cosson, M.P. 1992. Some problems related to the assessment of sperm motility in freshwater fish. J. Exp. Zool. 261: 122–131.

    Article  Google Scholar 

  • Billard, R., Cosson, J. and Crim, L.W. 1993. Motility of fresh and aged halibut sperm. Aquat. Living Resour. 6: 67–75.

    Google Scholar 

  • Billard, R., Cosson, J., Perchec, G. and Linhart, O. 1995. Biology of sperm and artificial reproduction in carp. Aquaculture 129: 95–112.

    Article  Google Scholar 

  • Büyükhatipoglu, S. and Holtz, W. 1984. Sperm output in rainbow trout (Salmo gairdneri) — effect of age, timing and frequency of stripping and presence of females. Aquaculture 37: 63–71.

    Article  Google Scholar 

  • Chambeyron, F. and Zohar, Y. 1990. A diluent for sperm cryopreservation of gilthead seabream,Sparus aurata. Aquaculture 90: 345–352.

    Article  Google Scholar 

  • Crim, L.W., Yao, Z. and Wang, Z. 1996. Reproductive mechanisms for the ocean pout (Macrozoarces americanus L.) a marine temperate fish, include internal fertilization and parental care of the eggs.In Proc. 5th Int. Symp. Reproductive Physiology of Fish. pp. 224–226. Edited by F.W. Goetz and P. Thomas. Austin, Texas.

  • Goodall, J.A., Blackshaw, A.W. and Capra, M.F. 1989. Factors affecting the activation and duration of motility of the spermatozoa of the summer whiting (Sillago ciliata). Aquaculture 77: 243–250.

    Article  Google Scholar 

  • Harmin, S.A. and Crim, L.W. 1993. Influence of gonadotropic hormone-releasing hormone analog (GnRH-A) on plasma sex steroid profiles and milt production in male winter flounder,Pseudopleuronectes americanus (Walbaum). Fish Physiol. Biochem. 10: 399–407.

    Article  CAS  Google Scholar 

  • Koya, Y., Munehara, H., Takano, K. and Takahashi, H. 1993. Effects of extracellular environments on the motility of spermatozoa in several marine sculpins with internal gametic association. Comp. Biochem. Physiol. 106A: 25–29.

    Article  Google Scholar 

  • Kruger, J.C., De, W., Smit, G.L., Van Vuren, J.H.J. and Ferreira, J.T. 1984. Some chemical and physical characteristics of the semen ofCyprinus carpio L. andOreochromis mossambicus. J. Fish Biol. 24: 263–272.

    Article  CAS  Google Scholar 

  • Lahnsteiner, F., Patzner, R.A. and Weismann, T. 1994. The testicular main ducts and the spermatic ducts in some cyprinid fishes — II. Composition of the seminal fluid. J. Fish Biol. 44: 459–467.

    CAS  Google Scholar 

  • Morisawa, M. 1985. Initiation mechanism of sperm motility at spawning in teleosts. Zool. Sci. 2: 605–615.

    CAS  Google Scholar 

  • Morisawa, M. and Suzuki, K. 1980. Osmolality and potassium ion: their roles in initiation of sperm motility in teleosts. Science 210: 1145–1147.

    PubMed  CAS  Google Scholar 

  • Morisawa, M., Suzuki, K., Shimizu, H., Morisawa, S. and Yasuda, K. 1983 Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes. J. Exp. Biol. 107: 95–103.

    PubMed  CAS  Google Scholar 

  • Munkittrick, K.R. and Moccia, R.D. 1987. Seasonal changes in the quality of rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma constituents. Aquaculture 64: 147–156.

    Article  Google Scholar 

  • Pavlov, D.A. and Radzikhovskaya, Ye K. 1991. Reproduction biology of white sea wolffish,Anarhichas marisalbi, based on experimental studies. Voprosy Ikhtiologii 31: 433–441.

    Google Scholar 

  • Pierce, A. 1992. Micro BCA Protein Assay Reagent Kit. Pierce Chemical Company, 1989.

  • Piironen, J. and HyvArinen, H. 1983. Composition of the milt of some teleost fishes. J. Fish Biol. 22: 351–361.

    Article  CAS  Google Scholar 

  • Redondo, M., Cosson, M.P., Cosson, J. and Billard, R. 1991.In vitro maturation of the potential for movement of carp spermatozoa Mol. Reprod. Dev. 29: 259–270.

    Article  Google Scholar 

  • Scott, A.P. and Baynes, S.M. 1980. A review of the biology, handling and storage of salmonid spermatozoa. J. Fish Biol. 17: 707–739.

    Article  Google Scholar 

  • Stoss, J. 1983. Fish gamete preservation and spermatozoan physiology.In Fish Physiology. Vol. IX B, pp. 305–350. Edited by W.S. Hoar, D.J. Randall and E.M. Donaldson. Academic Press, New York.

    Google Scholar 

  • Suquet, M., Dorange, G., Omnes, M.H., Normant, Y., Roux, A.Le and Fauvel, C. 1993. Composition of the seminal fluid and ultrastructure of the spermatozoa of turbot (Scophthalmus maximum). J. Fish Biol. 42: 509–516.

    Article  Google Scholar 

  • Yao, Z. and Crim, L.W. 1995. Spawning of ocean pout (Macrozoarces americanus L.): evidence in favour of internal fertilization of eggs. Aquaculture 130: 361–372.

    Article  Google Scholar 

  • Yao, Z., Emerson, C.J. and Crim, L.W. 1995. Ultrastructure of the spermatozoa and eggs of the ocean pout (Macrozoarces americanus L.): an internally fertilizing marine fish. Mol. Reprod. Dev. 42: 58–64.

    Article  PubMed  CAS  Google Scholar 

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  1. Ocean Sciences Centre, Memorial University of Newfoundland, St. John's, NF, AIC 557, Canada

    Z. Wang & L. W. Crim

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  1. Z. Wang
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  2. L. W. Crim
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Wang, Z., Crim, L.W. Seasonal changes in the biochemistry of seminal plasma and sperm motility in the ocean pout,Macrozoarces americanus . Fish Physiol Biochem 16, 77–83 (1997). https://doi.org/10.1007/BF00004542

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  • DOI: https://doi.org/10.1007/BF00004542

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