Abstract
Two year old black porgy (Acanthopagrus schlegeli) fed a diet containing 4.0 mg kg−1 of estradiol-17β (E2) for 5 months had significantly lower GSI than the control group during the spawning season. E2 suppressed testicular development, spermiation and plasma testosterone (T) and 11-ketotestosterone (11-KT) and stimulated ovarian development, vitellogenesis and sex reversal. Spermiation in the control group occurred in January and February with the concentrations of 1.08–1.36 × 1010 sperm ml−1 of milt. Higher plasma T and 11-KT, but lower E2 levels were detected in the spermiating fish (control group). Higher plasma E2 levels were detected in the sex reversing black porgy during the pre-spawning season. A sharp rise in plasma 11-KT and a drop in T levels were detected in spermiating fish (control group) from January to February. Plasma 11-KT levels correlated with the testicular development and spermiation. The data suggest that E2 plays an important role in controlling the sex reversal of black porgy.
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References cited
Billard, R. 1978. Testicular feedback on the hypothalamus-pituitary axis in rainbow trout (Salmo gairdneri R.). Ann. Biol. Anim. Biochem. Biophys. 18: 813–818.
Chang, C.F. and Yueh, W.S. 1990. Annual cycle of gonadal histology and steroid profiles in the juvenile males and adult females of the protandrous black porgy, Acanthopagrus schlegeli. Aquaculture 91: 179–196.
Chang, C.F., Yueh, W.S. and Lee, M.F. 1991. Effects of LHRH-A and HCG on the steroid profiles of bisexual and mature male and female protandrous black porgy, Acanthopagrus schlegeli. Aquaculture 92: 83–92.
Chang, C.F., Lee, M.F. and Chen, G.R. 1994. Estradiol-17β associated with the sex reversal in protandrous black porgy, Acanthopagrus schlegeli. J. Exp. Zool. 268: 53–58.
Chang, C.F. and Shih, T.W. 1995. Reproductive cycle of ovarian development and vitellogenin profiles in the freshwater prawn, Macrobrachium rosenbergii. Invert. Reprod. Dev. 27: 11–20.
Chang, C.F., Yueh, W.S., Lee, M.F. and Schally, A.V. 1995. A microencapsulated analog of LH-RH accelerates maturation but without stimulating sex reversal in the protandrous black porgy, Acanthopagrus schlegeli. Reprod. Nutr. Dev. 35: (In press).
Cochran, R.C. 1992. In vivo and in vitro evidence for the role of hormones in fish spermatogenesis. J. Exp. Zool. 261: 143–150.
de Vlaming, V.L., Wiley, H.S., Delahuntry, G. and Wallace, R.A. 1980. Goldfish (Carassius auratus) vitellogenin: induction, properties and relationship to yolk proteins. Comp. Biochem. Physiol. 67: 613–623.
England, B.G., Niswender, G.D. and Midgley, A.R.Jr. 1974. Radioimmunoassay of estradiol-17β without chromatograph. J. Clin. Endocrinol. Metab. 38: 42–50.
Fostier, A. Billard, R., Breton, B., Legendre, M. and Marlot, S. 1982. Plasma 11-oxotestosterone and gonadotropin during the beginning of spermiation in rainbow trout (Salmo gairdneri R.). Gen. Comp. Endocrinol. 46: 428–434.
Fostier, A., Jalabert, B., Billard, R., Breton, B. and Zohar, Y. 1983. The gonadal steroids. In Fish Physiology. Vol. IXA, pp. 277–372. Edited by W.S. Hoar, D.J. Randall and E.M. Donaldson. Academic Press, New York.
Godwin, J.R. and Thomas, P. 1993. Sex change and steroid profiles in the protandrous anemonefish Amphiprion melanopus (Pomacentridae, Teleostei). Gen. Comp. Endocrinol. 91: 145–157.
Guiguen, Y., Jalabert, B., Thouard, E. and Fostier, A.G. 1993. Change in plasma and gonadal steroid hormone relation to the reproductive cycle and the sex inversion process in the protandrous seabass, Lates calcarifer. Gen. Comp. Endocrinol. 92: 327–338.
Hansson, V., Calandra, R., Purvis, K., Ritzen, M. and French, F.S. 1976. Hormonal regulation of spermatogenesis. Vit. Horm. 34: 187–214.
Hunter, G.A. and Donaldson, E.M. 1983. Hormonal sex control and its application to fish culture. In Fish Physiology. Vol. IXB, pp. 223–303. Edited by W.S. Hoar, D.J. Randall and E.M. Donaldson. Academic Press, New York.
Kime, D.E. and Dolben, I.P. 1985. Hormonal changes during induced ovulation of the carp, Cyprinus carpio. Gen. Comp. Endocrinol. 58: 137–149.
Kime, D.E., Lone, K.P. and Al-Marzouk, A. 1991. Seasonal changes in serum steroid hormones in a protandrous teleost, the sobaity (Sparidentex hasta Valenciennes). J. Fish Biol. 39: 745–753.
Kime, D.E. and Manning, N.J. 1982. Seasonal pattern of free and conjugated androgens in the brown trout, Salmo trutta. Gen. Comp. Endocrinol. 48: 222–231.
Kishida, M., Anderson, T.R. and Specker, J.L. 1992. Induction by 17β-estradiol of vitellogenin in striped bass (Morone saxatilis): characterization and quantification in plasma and mucus. Gen. Comp. Endocrinol. 88: 29–39.
Kramer, C.R., Koulish, S. and Bertacchi, P.L. 1988. The effects of testosterone implants on ovarian morphology in the bluehead wrasse, Thalassonia bifasciatium (Bloch) (Teleostei: Labridae). J. Fish Biol. 32: 397–407.
Lee, M.F., Chang, C.F., Huang, J.D. and Schally, A.V. 1993. Microencapsulated LH-RH analog accelerated spermiation in protandrous black porgy, Acanthopagrus schlegeli. Bull. Inst. Zool. Acad. Sin. 32: 32–38.
Miura, T., Yamauchi, K., Nayahama, Y. and Takahashi, H. 1991. Induction of spermatogenesis in male Japanese eel, Anguilla japonica, by a single injection of human chorionic gonadotropin. Zool. Sci. 8: 63–73.
Miura, T., Yamauchi, K., Takahashi, H. and Nagahama, Y. 1991. Hormonal induction of all stages of spermatogenesis in vitro in the male Japanese eel (Anguilla japonica). Proc. Nat. Acad. Sci. U.S.A. 88: 5774–5778.
Ng, T.B. and Idler, D.R. 1983. Yolk formation and differentiation in teleost fishes. In Fish Physiology. Vol. IXA, pp. 373–404. Edited by W.S. Hoar, D.J. Randall and E.M. Donaldson. Academic Press, New York.
Pandey, S. 1969. Effects of methyl-testosterone on the testis and secondary sex characters of the hypophysectomized adult guppy Poecilia reticulata Peter. Can. J. Zool. 47: 783–786.
Scott, A.P., Bye, V.J., Baynes, S.M. and Springate, J.R.C. 1980. Seasonal variations in plasma concentrations of 11-ketotestosterone and testosterone in male rainbow trout, Salmo gairdneri Richardson. J. Fish Biol. 17: 495–505.
Steel, R.G.D. and Torrie, J.H. 1980. Principles and Procedures of Statistics. McGraw-Hill Book Co., New York.
Sundararaj, B.I. and Nayyar, S.K. 1967. Effects of exogenous gonadotrophins and gonadal hormones on the testis and seminal vesicles of hypophysectomized catfish, Heteropneustes fossilis (Block). Gen. Comp. Endocrinol. 8: 403–416.
Tang, F., Chan, S.T.H. and Lofts, B. 1974. Effects of steroid hormones on the process of natural sex reversal in the ricefield eel, Monopterus albus (Zuiew). Gen. Comp. Endocrinol. 24: 227–241.
Ueda, H., Kambegawa, A. and Nagahama, Y. 1985. Involvement of gonadotrophin and steroid hormones in spermiation in the amago salmo, Oncorhynchus rhodurus, and goldfish, Carassius auratus. Gen. Comp. Endocrinol. 59: 24–30.
Yamamoto, T. 1969. Sex differentiation. In Fish Physiology. Vol. 3, pp. 117–175. Edited by W.S. Hoar and D.J. Randall. Academic Press, New York.
Yamazaki, F. 1983. Sex control and manipulation in fish. Aquaculture 33: 329–354.
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Chang, CF., Lau, EL. & Lin, BY. Estradiol-17β suppresses testicular development and stimulates sex reversal in protandrous black porgy, Acanthopagrus schlegeli . Fish Physiol Biochem 14, 481–488 (1995). https://doi.org/10.1007/BF00004348
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DOI: https://doi.org/10.1007/BF00004348