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Ascorbate-2-sulfate as a dietary vitamin C source for atlantic salmon (Salmo salar): 1. Growth, bioactivity, haematology and humoral immune response

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Abstract

The present experiment shows that ascorbate-2-sulfate (AS) is not equivalent to ascorbic acid (AA) as a dietary vitamin C source for Atlantic salmon (Salmo salar). Within reasonable feed supplemental levels AS does not provide the tissues with adequate supplies of vitamin C to secure optimal physiological functions as demonstrated by biochemical and haematological analyses.

AS could not be detected in the liver of fish fed either AA or AS, nor in vitamin C — deprived salmon, suggesting that AS is not the natural storage form of vitamin C in this species.

There were no significant differences in antibody production against a soluble artificial antigen (NIP11-LPH) in fish fed 500 and 5000 mg AA/Kg dry diet or equivalent amounts of AS during a period of six weeks at a water temperature of 7.2°C.

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References cited

  • Albrektsen, S., Lie, Ø. and Sandnes, K. 1988. Ascorbyl palmitate as a dietary vitamin C source for Rainbow trout (Salmo gairdneri). Aquaculture 71: 359–368.

    Article  CAS  Google Scholar 

  • Baker III, E.M., Hammer, D.C., March, S.C., Tolbert, B.M. and Canham, J.E. 1971. Ascorbate sulfate: A urinary metabolite of ascorbic acid in man. Science 173: 826–827.

    PubMed  CAS  Google Scholar 

  • Bell, G.R., Higgs, D.A. and Traxler, G.S. 1984. The effect of dietary ascorbate, zinc, and manganeseon the development of experimentally induced bacterial kidney disease in Sockeye salmon (Oncorhynchus nerka). Aquaculture 36: 293–311.

    Article  CAS  Google Scholar 

  • Benitez, L.V. and Halver, J.E. 1982. Ascorbic acid sulfate sulfohydrolase (C2 sulfatase): The modulator of cellular levels of L-ascorbic acid in rainbow trout. Proc. Natl. Acad. Sci. 79: 5445–5449.

    Article  PubMed  CAS  Google Scholar 

  • Blazer, V.S. 1982. The effect of marginal deficiencies of ascorbic acid and alpha-tocopherol on the natural resistance and immune response of rainbow trout (Salmo gairdneri). Ph.D. dissertation. University of Rhode Island, U.S.A.

  • Durve, V.S. and Lovell, R.T. 1982. Vitamin C and disease resistance in channel catfish (Ictalurus punctatus). Can. J. Fish. Aquat. Sci. 39: 948–951.

    CAS  Google Scholar 

  • Espelid, S., Hjelmeland, K. and Jørgensen, T. 1987. The specificity of Atlantic salmon antibodies made against the fish pathogen Vibrio salmonicida, establishing the surface protein VS-P1 as the dominating antigen. Develop. Comp. Immun. 11: 529–537.

    Article  CAS  Google Scholar 

  • Halver, J.E., Smith, R.R., Tolbert, B.M. and Baker, E.M. 1975. Utilization of ascorbic acid in fish. Ann. N.Y. Acad. Sci. 258: 81–102.

    PubMed  CAS  Google Scholar 

  • Hilton, J.W., Cho, C.Y. and Slinger, S.J. 1978. Effect of graded levels of supplemental ascorbic acid in practical diets fed to rainbow trout (Salmo gairdneri). J. Fish. Res. B. Can. 35: 431–436.

    CAS  Google Scholar 

  • Hornig, D. 1975. Distribution of ascorbic acid, metabolites and analogues in man and animals. Ann. N.Y. Acad. Sci. 258: 103–118.

    PubMed  CAS  Google Scholar 

  • Hornig, D., Glatthaar, B. and Moser, U. 1984. General aspects of ascorbic acid function and metabolism. In: Ascorbic acid in domestic animals. pp. 3–24. Edited by I. Wegger, F.J. Tagwerker and J. Moustgaard. The Royal Danish Agricultural Society, Copenhagen.

    Google Scholar 

  • Killie, J-E.A. 1987. Humoral immunrespons hos Atlantisk laks (Salmo salar) studert ved hjelp av haptenisert antigen, M.D. Thesis, University of Tromsø, Norway (in Norwegian).

  • Kuenzig, W., Avenia, R. and Kamm, J.J. 1974. Studies on the antiscorbutic activity of ascorbate 2-sulfate in the Guinea pig. J. Nutr. 104: 952–956.

    PubMed  CAS  Google Scholar 

  • Landolt, M.L. 1989. The relationship between diet and the immune responses of fish. Aquaculture (in press).

  • Li, Y. and Lovell, R.T. 1985. Elevated levels of dietary ascorbic acid increase immune responses in Channel catfish. J. Nutr. 115: 123–131.

    PubMed  CAS  Google Scholar 

  • Lie, Ø., Waagbø, R. and Sandnes, K. 1988. Growth and chemical composition of adult Atlantic salmon (Salmo salar) fed dry and silage based diets. Aquaculture 69: 343–353.

    Article  Google Scholar 

  • Lovell, R.T. 1984. Ascorbic acid metabolism in fish. In: Ascorbic acid in domestic animals. pp. 196–205. Edited by I. Wegger, F.J. Tagwerker and J. Moustgaard. The Royal Danish Agricultural Society, Copenhagen.

    Google Scholar 

  • Murai, T., Andrews, J.W. and Bauernfeind, J.C. 1978. Use of L-ascorbic acid, ethocel coated ascorbic acid and ascorbate-sulfate in diets for channel catfish, Ictalurus punctatus. J. Nutr. 108: 1761–1766.

    PubMed  CAS  Google Scholar 

  • Machlin, L.J., Garcia, F., Kuenzig, W., Richter, C.B., Spiegel, H.E. and Brin, M. 1976. Lack of antiscorbutic activity of ascorbate-2-sulfate in the rhesus monkey. Am. J. Clin. Nutr. 29: 825–831.

    PubMed  CAS  Google Scholar 

  • Måge, A., Waagbø, R., Olson, O., Julshamn, K. and Sandnes, K., 1990. Ascorbate-2-sulfate as a vitamin C source for Atlantic salmon (Salmo salar): 2. Effects of dietary level and immunization on the metabolism of trace elements. Fish. Phys. Biochem. 8 (6): 429–436.

    Google Scholar 

  • Navarre, O. and Halver, J.E. 1989. Disease resistance and humoral antibody production in rainbow trout fed high levels of vitamin C. aquaculture 79: 207–221.

    Article  CAS  Google Scholar 

  • Omaye, S.T., Tillotson, J.A. and Sauberlich, H.E. 1982. Metabolism of L-ascorbic acid in the monkey. In: Ascorbic acid: Chemistry, Metabolism, and Uses. pp. 317–334. Edited by P.A. Seib and B.M. Tolbert. American Chemical Society, Washington, D.C.

    Google Scholar 

  • Rose, R.C. 1981. Transport and metabolism of water-soluble vitamins in intestine. Am. J. Physiol. 240: G97-G101.

    PubMed  CAS  Google Scholar 

  • Sandnes, K., Lie, Ø. and Waagbø, R. 1988. Normal ranges of some blood chemistry parameters in adult farmed Atlantic salmon, Salmo salar. J. Fish. Biol. 32: 129–136.

    Article  CAS  Google Scholar 

  • Schuep, W., Vuilleumier, J.P., Gysel, D. and Hess, D. 1984. Determination of ascorbic acid in body fluids, tissues and feedstuffs. In: Ascorbic Acid in Domestic Animals. pp. 50–55. Edited by I. Wegger, F.J. Wagwerker and J. Moustgaard. The Royal Danish Agricultural Society, Copenhagen.

    Google Scholar 

  • Schuep, W., Marmet, Jurg and Studer, W. 1989. Stability of ascorbate-2-sulfate in trout feed measured by HPLC. Aquaculture 79: 249–258.

    Article  Google Scholar 

  • Soliman, A.K., Jauncey, K. and Roberts, R.J. 1986. The effect of dietary ascorbic acid supplementation on hatchability, survival rate and fry performance in Oreochromis mossambicus (Peters). Aquaculture 59: 197–208.

    Article  CAS  Google Scholar 

  • Tillotson, J.A. and McGown, E.L. 1981. The relationship of the urinary ascorbate metabolites to specific levels of ascorbate supplementation in the monkey. Amer. J. Clin. Nutr. 34: 2405–2411.

    PubMed  CAS  Google Scholar 

  • Tsujimura, M., Haruhisa, Y., Hasegawa, T., Suzuki, T., Takamasa, K., Suiva, T. and Kitamura, S. 1978. Studies on vitamin C activity of ascorbic acid-2-sulfate on the feeding test of newborn rainbow trout. Vitamins (Japan) 52: 35–44.

    CAS  Google Scholar 

  • Tsujimura, M., Fukuda, T., Kasai, T. and Kitamura, S. 1981. Hydrolysis of L-ascorbic acid 2-sulfate to L-ascorbic acid in freshwater fishes. Bull. Jap. Soc. Sci. Fish. 47: 435.

    CAS  Google Scholar 

  • Tsujimura, M., Fukuda, T. and Kasai, T. 1982. Studies on the excretion of ascorbic acid 2-sulfate and total vitamin C into human urine after oral administration of ascorbic acid 2-sulfate. J. Nutr. Sci. Vitaminol. 28: 467–476.

    PubMed  CAS  Google Scholar 

  • Tucker, B.W. 1983. Studies on vitamin C metabolism in rainbow trout. Ph.D. dissertation, University of Washington, U.S.A.

  • Tucker, B.W. and Halver, J.E. 1984. Distribution of ascorbate-2-sulfate and distribution, halv-life and turnover rates of (1-14C)ascorbic acid in rainbow trout. J. Nutr. 114: 991–1000.

    PubMed  CAS  Google Scholar 

  • Tucker, B.W. and Halver, J.E. 1986. Utilization of ascorbate-2-sulfate in fish. Fish Physiol. Biochem. 2: 151–160.

    Article  CAS  Google Scholar 

  • Waagbø, R., Thorsen, T. and Sandnes, K. 1989. Role of dietary ascorbic acid in vitellogenesis in rainbow trout (Salmo gairdneri). Aquaculture 80: 301–314.

    Article  Google Scholar 

  • Wahli, T., Meier, W. and Pfister, K. 1986. Ascorbic acid induced immune-mediated decrease in mortality in Ichthyophthirius multifiliis infected rainbow-trout (Salmo gairdneri). Acta Tropica 43: 287–289.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Nutrition, Directorate of Fisheries, P.O. Box 1900, N-5024, Bergen, Norway

    K. Sandnes & R. Waagbø

  2. Aquaculture Station Matre, N-5198, Matredal, Norway

    T. Hansen

  3. The Foundation of Applied Research at the University of Tromsø, P.O. Box 2806, Elverhøy, N-9001, Tromsø, Norway

    J-E. A. Killie

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  1. K. Sandnes
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  2. T. Hansen
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  3. J-E. A. Killie
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  4. R. Waagbø
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Sandnes, K., Hansen, T., Killie, JE.A. et al. Ascorbate-2-sulfate as a dietary vitamin C source for atlantic salmon (Salmo salar): 1. Growth, bioactivity, haematology and humoral immune response. Fish Physiol Biochem 8, 419–427 (1990). https://doi.org/10.1007/BF00003398

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