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Soil Enzymes and Their Role in Nutrient Cycling

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Structure and Functions of Pedosphere

Abstract

Soil is a dynamic living non-renewable resource that acts as an interface between agriculture and environment. Soil enzymes play critical role in soil processes ranging from biochemical reactions in plants, animals, and microbes to decomposition of organic matter, soil structure stabilization and nutrients cycling. Different microbes and enzymes are involved in cycling of carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) in an ecosystem. The nutrient cycles function as a result of biological and physico-chemical reactions in soil. The nutrients cycling in an ecosystem are primarily governed by soil microbes and enzyme activities. The biochemical reactions of these cycles are accomplished by enzymes such as amidases, arylsulphatase, cellulases, dehydrogenase, glucosidases, laccase, phosphatases, and urease. These enzymes serve as biological indicators that help to identify variations in soil physical, chemical, and biological properties. Enzymes respond faster to soil management practices long before other soil quality indicator changes are detectable and their method of detection is simple, easy, and quick. In this chapter, we will discuss about the major enzymes involved in the cycling of C, N, P, S, and their mechanisms of action, role in maintaining soil health and factors that affect their activities in soil.

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References

  • Abbott KC, Karst J, Biederman LA, Borrett SR, Hastings A, Walsh V, Bever JD (2015) Spatial heterogeneity in soil microbes alters outcomes of plant competition. PLoS One 10(5):10–25

    Article  CAS  Google Scholar 

  • Acosta-Martínez V, Tabataba MA (2011) Phosphorus cycle enzymes. In: Dick RP (ed) Methods of soil enzymology. SSSA Book Soil Science Society of America, Madison, pp 161–183

    Google Scholar 

  • Alef K, Nannipieri P (1995) Methods in applied soil microbiology and biochemistry. Academic, London

    Google Scholar 

  • Alkorta I, Aizpurua A, Riga P, Albizu I, Amezaga I, Garbisu C (2003) Soil enzyme activities as biological indicators of soil health. Rev Environ Health 18(1):65–73

    Article  PubMed  Google Scholar 

  • Antonious GF (2003) Impact of soil management and two botanical insecticides on urease and invertase activity. J Environ Sci Health Part B 38(4):479–488

    Article  CAS  Google Scholar 

  • Baldrian P (2006) Fungallaccases: occurrence and properties. FEMS Microbiol Rev 30:215–242

    Article  CAS  PubMed  Google Scholar 

  • Baldrian P (2009) Microbial enzyme-catalyzed processes in soils and their analysis. Plant Soil Environ 55(9):370–378

    Article  CAS  Google Scholar 

  • Bandick AK, Dick RP (1999) Field management effects on soil enzyme activities. Soil Biol Biochem 31:1471–1479

    Article  CAS  Google Scholar 

  • Baoyi J, Hao H, Zhao Y, Xinyuan M, Liu K, Li C (2014) Effects of deep tillage and straw returning on soil microorganism and enzyme activities. Sci World J 2014:451493

    Google Scholar 

  • Botha AM, Nagel MAC, Westhuizen V, Botha FC (1998) Chitinaseisoenzymes in near-isogenic wheat lines challenged with Russian wheat aphid, exogenous ethylene, and mechanical wounding. Bot Bull Acad Sin 39:99–106

    CAS  Google Scholar 

  • Burns RG, DeForest JL, Marxsen J, Sinsabaugh RL, Stromberger ME, Wallenstein MD, Weintraub MN, Zoppini A (2013) Soil enzymes in a changing environment: Current knowledge and future directions. Soil Biol Biochem 58:216–234

    Article  CAS  Google Scholar 

  • Caldwell BA (2005) Enzyme activities as a component of soil biodiversity: a review. Pedobiologia 49:637–644

    Article  CAS  Google Scholar 

  • Chebrou H, Bigey F, Arnaud A, Galzy P (1996) Study of the amidase signature group. Biochim Biophys Acta 1298:285–293

    Article  CAS  PubMed  Google Scholar 

  • Claus H, Filip Z (1988) Behavior of phenoloxidases in the presence of clays and other soil-related adsorbents. Appl Microbiol Biotechnol 28:506–511

    Article  CAS  Google Scholar 

  • Collinge DB, Kragh KM, Mikkelsen JD, Nielsen KK, Rasmussen U, Vad K (1993) Plant chitinases. Plant J 3(1):31–40. https://doi.org/10.1046/j.1365-313x.1993.t01-1-00999.x

    Article  CAS  PubMed  Google Scholar 

  • Dale VH, Beyeler SC (2001) Challenges in the development and use of ecological indicators. Ecol Indic 1:3–10

    Article  Google Scholar 

  • Dedeken M, Voets JP (1965) Research on the metabolism of amino acids in the soil. I. The metabolism of glycine, alanine, aspartic acid and glutaminic acid. Ann Inst Pasteur (Paris) 109(3):103–111

    Google Scholar 

  • Dotaniya ML, Aparna K, Dotaniya CK, Singh M, Regar KL (2019) Role of soil enzymes in sustainable crop production. Enzymes Food Biotechnol 2019:569–589

    Article  Google Scholar 

  • Elbl J, Makova J, Javorekova S, Medo J, Kint A, Losak T, Lukas V (2019) Response of microbial activities in soil to various organic and mineral amendments as an indicator of soil quality. Agronomy 9:485. https://doi.org/10.3390/agronomy9090485

    Article  CAS  Google Scholar 

  • Esen A (1993) β-Glucosidases. In: Esen A (ed) Î’-Glucosidases: biochemical and molecular biology. American Chemical Society, Washington, DC, pp 1–14

    Chapter  Google Scholar 

  • Fournand D, Arnaud A (2001) Aliphatic and enantio selective amidases: from hydrolysis to acyl transfer activity. J Appl Microbiol 91:381–393

    Article  CAS  PubMed  Google Scholar 

  • Gelsomino A, Badalucco L, Landi LCG (2006) Soil carbon, nitrogen and phosphorus dynamics as affected by solarization alone or combined with organic amendment. Plant Soil 279:307–325

    Article  CAS  Google Scholar 

  • Gil-Sotres F, Trasar-Cepeda C, Leirós M, Seoane S (2005) Different approaches to evaluating soil quality using biochemical properties. Soil Biol Biochem 37:877–887

    Article  CAS  Google Scholar 

  • Glick BR (2010) Using soil bacteria to facilitate phytoremediation. Biotechnol Adv 28(3):367–374

    Article  CAS  PubMed  Google Scholar 

  • Gomez L, Allona I, Casado R, Aragoncillo C (2002) Seed chitinases. Seed Sci Res 12:217–230

    Article  CAS  Google Scholar 

  • Gu Y, Wag P, Kong C (2009) Urease, invertase, dehydrogenase and polyphenoloxidase activities in paddy soils influenced by allelophatic rice variety. Eur J Soil Biol 45:436–441

    Article  CAS  Google Scholar 

  • Gunjal AB, Waghmode MS, Patil NN, Nawani NN (2019) Significance of soil enzymes in agriculture. In: Smart bioremediation technologies. Springer, New York, pp 159–168

    Chapter  Google Scholar 

  • Gupta RD, Bhardwaj KKR (1990) Phosphatase and urease enzymatic activities in some soil profiles of north west Himalayas. J Indian Soc Soil Sci 38(4):756–759

    Google Scholar 

  • Hatakka A (1994) Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation. FEMS Microbiol Rev 13:125–135

    Article  CAS  Google Scholar 

  • Jian S, Li J, Chen J, Wang G, Mayes MA, Dzantor KE (2016) Soil extracellular enzyme activities, soil carbon and nitrogen storage under nitrogen fertilization: A meta-analysis. Soil Biol Biochem 101:32–43

    Article  CAS  Google Scholar 

  • Joshi S, Mohapatra B, Mishra JPN (2018) Microbial soil enzymes: implications in the maintenance of rhizosphere ecosystem and soil health. In: Adhya TK et al (eds) Advances in soil microbiology: recent trends and future prospects, microorganisms for sustainability. Springer, Singapore

    Google Scholar 

  • Kandeler E, Poll C, Frankenberger WT, Tabatabai MA (2011) Nitrogen cycle enzymes. In: Dick RP (ed) Methods of soil enzymology. SSSA Book Series, Madison, pp 211–245

    Google Scholar 

  • Killham K (2002) Bioindicators and sensors of soil health and the application of geostatistics. In: Burns RG, Dick RP (eds) Enzymes in the environment: activity, ecology and applications. Marcel Dekker, Inc., New York

    Google Scholar 

  • Klose S, Bilen S, Tabatabai MA, Dick WA (2011) Sulfur cycle enzymes methods of soil enzymology. SSSA Book Series, New York

    Google Scholar 

  • Liu C, Song Y, Dong X, Wang X, Ma X, Zhao G, Zang S (2021) Soil enzyme activities and their relationships with soil C, N, and P in peatlands from different types of permafrost regions northeast china. Front Environ Sci 9:670769. https://doi.org/10.3389/fenvs.2021.670769

    Article  Google Scholar 

  • Lynd LR, van Zyl WH, McBride JE, Laser M (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol 16:577–583

    Article  CAS  PubMed  Google Scholar 

  • Majchrzak L, Sawinska Z, Natywa M, Skrzypczak G, WoÅ‚oszyn R (2016) Impact of different tillage systems on soil dehydrogenase activity and spring wheat infection. J Agric Sci Technol 18:1871–1881

    Google Scholar 

  • Makoi JHJR, Ndakidemi PA (2008) Selected soil enzymes: examples of their potential roles in the ecosystem. Afr J Biotechnol 7(3):181–191

    CAS  Google Scholar 

  • Mangalassery S, Mooney SJ, Sparkes DL, Fraser WT, Sjögersten S (2015) Impacts of zero tillage on soil enzyme activities, microbial characteristics and organic matter functional chemistry in temperate soils. Eur J Soil Biol 68:9–17

    Article  CAS  Google Scholar 

  • Merino C, Godoy R, Matus F (2016) Soil enzymes and biological activity at different levels of or ganic matter stability. J Soil Sci Plant Nutr 16:14–30

    CAS  Google Scholar 

  • McGill WB, Colle CV (1981) Comparative aspects of cycling of organic C, N, S and P through soil organic matter. Geoderma 26:267–286

    Article  CAS  Google Scholar 

  • Moghimian N, Hosseini SM, Kooch Y, Darki BZ (2017) Impacts of changes in land use/cover on soil microbial and enzyme activity. Catena 157:407–414

    Article  CAS  Google Scholar 

  • Murphy CD (2016) Microbial degradation of fluorinated drugs: biochemical pathways, impacts on the environment and potential applications. Appl Microbiol Biotechnol 100(6):2617–2627

    Article  CAS  PubMed  Google Scholar 

  • Nannipieri P, Giagnoni L, Landi L, Renella G (2011) Role of phosphatase enzymes in soil. In: Beunemann EK et al (eds) Phosphorus in action, vol 26. Springer, Berlin. https://doi.org/10.1007/978-3-642-15271-9_9

    Chapter  Google Scholar 

  • Pace HC, Brenner C (2001) The nitrilase superfamily: classification, structure and function. Genome Biol 2(1):REVIEWS0001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pancholy SK, Rice EL (1973) Soil enzymes in relation to old field succession: amylase, cellulase, invertase, dehydrogenase and urease. Soil Sci Soc Am Proc 37:47–50

    Article  CAS  Google Scholar 

  • Patle PN, Navnage NP, Barange PK (2018) Fluorescein diacetate (FDA): measure of total microbial activity and as indicator of soil quality. J Curr Microbiol Appl Sci 7(6):2103–2107. https://doi.org/10.20546/ijcmas.2018.706.249

    Article  CAS  Google Scholar 

  • Pertsovich SI, Guranda DT, Podchernyaev DA, Yanenko AS, Svedas VK (2005) Aliphatic amidase from Rhodococcusrhodochrous M8 is related to the nitrilase/cyanide hydratase family. Biochemistry 70:1280–1287

    CAS  PubMed  Google Scholar 

  • Prober SM, Leff JW, Bates ST, Borer ET, Firn J, Harpole WS, Lind EM, Seabloom EW, Adler PB, Bakker JD (2015) Plant diversity predicts beta but not alpha diversity of soil microbes across grasslands worldwide. Ecol Lett 18:85–95

    Article  PubMed  Google Scholar 

  • Rajkumar M, Prasad MNV, Swaminathan S, Freitas H (2013) Climate change driven plant–metal–microbe interactions. Environ Int 53:74–86

    Article  CAS  PubMed  Google Scholar 

  • Raju MN, Golla N, Vengatampalli R (2017) Soil protease. Springer, Cham

    Google Scholar 

  • Reddy GB, Faza A, Bennett R Jr (1987) Activity of enzymes in rhizosphere and nonrhizosphere soils amended with sludge. Soil Biol Biochem 19(2):203–205

    Article  Google Scholar 

  • Roberto G-R, Victoria O, Benjamín V, Belen HM, Reyes P-S, Liébanas G, Juan L, José C (2009) Soil enzymes, nematode community and selected physico-chemical properties as soil quality indicators in organic and conventional olive oil farming: influence of seasonality and site features. Appl Soil Ecol 41:305–314

    Article  Google Scholar 

  • Ross DJ (1975) Studies on a climosequence of soils in tussock grasslands-5: invertase and amylase activities of top soils and their relationships with other properties. N Z J Sci 18:511–518

    CAS  Google Scholar 

  • Sahai AS, Manocha MS (1993) Chitinases of fungi and plants: their involvement in morphogenesis and host-parasite interaction. FEMS Microbiol Rev 11:317–338

    Article  CAS  Google Scholar 

  • Saiya-Cork K, Sinsabaugh R, Zak D (2002) The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol Biochem 34(9):1309–1315

    Article  CAS  Google Scholar 

  • Sánchez-Monedero MA, Mondini C, Cayuela ML, Roig A, Contin M, De Nobili M (2008) Fluorescein diacetate hydrolysis, respiration and microbial biomass in freshly amended soils. Biol Fertil Soils 44:885–890. https://doi.org/10.1007/s00374-007-0263-1

    Article  CAS  Google Scholar 

  • Schmidt G, Laskowski MSK (1961) Phosphate csttr cleavage (survey), 2nd edn. Academic, New York

    Google Scholar 

  • Schnürer J, Rosswall T (1982) Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter. Appl Environ Microbiol 43:1256–1261

    Article  PubMed  PubMed Central  Google Scholar 

  • Shah T, Lateef S, Noor MA (2020) Carbon and nitrogen cycling in agroecosystems: an overview. In: Datta R et al (eds) Carbon and nitrogen cycling in soil. Springer, Singapore

    Google Scholar 

  • Sherene T (2017) Role of soil enzymes in nutrient transformation: a review. Biol Bull 3(1):109–131

    Google Scholar 

  • Singh M, Meena SC, Jain RK (2016) Application of chitinase for sustainable agriculture. Adv Life Sci 5(24):1131–1138

    Google Scholar 

  • Song Y, Song C, Shi F, Wang M, Ren J, Wang X (2019) Linking plant community composition with the soil C pool, N availability and enzyme activity in boreal Peatlands of Northeast China. Appl Soil Ecol 140:144–154. https://doi.org/10.1016/j.apsoil.2019.04.019

    Article  Google Scholar 

  • Strong PJ, Claus H (2011) Laccase: a review of its past and its future in bioremediation. Crit Rev Environ Sci Technol 41(4):373–434. https://doi.org/10.1080/10643380902945706

    Article  Google Scholar 

  • Suarez V, Staehelin C, Arango R, Holtorf H, Hofsteenge J, Meins F Jr (2001) Substrate specificity and antifungal activity of recombinant tobacco class I chitinases. Plant Mol Biol 45:609–618

    Article  CAS  PubMed  Google Scholar 

  • Tabatabai MA (1994) Soil enzymes. In: Weaver RW et al (eds) Methods of soil analysis, part 2. Microbiological and biochemical properties. Springer, New York, pp 775–833

    Google Scholar 

  • Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307

    Article  CAS  Google Scholar 

  • Tabatabai MA, Dick WA (1979) Distribution and stability of pyrophosphatase in soils. Soil Biol Biochem 11:655–659

    Article  CAS  Google Scholar 

  • Tarafdar JC, Jungk A (1987) Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol Fertil Soils 3:199–204

    Article  CAS  Google Scholar 

  • Thoma JA, Spradlin JE, Dygerd S (1971) Plant and animal amylases. Academic, New York

    Book  Google Scholar 

  • Thurston CF (1994) The structure and function of fungal laccases. Microbiology 140:19–26

    Article  CAS  Google Scholar 

  • Ullah S, Ai C, Huang S, Zhang J, Jia L, Ma J, Zhou W, He P (2019) The responses of extracellular enzyme activities and microbial community composition under nitrogen addition in an upland soil. PLoS ONE 14(9):e0223026

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang SL, Wang CY, Huang TY (2008) Microbial reclamation of squid pen for the production of a novel extracellular serine protease by Lactobacillus paracaseisubspparacasei TKU012. Bioresour Technol 99:3411–3417

    Article  CAS  PubMed  Google Scholar 

  • Wang H, Wu J, Li G, Yan L (2020) Changes in soil carbon fractions and enzyme activities under different vegetation types of the northern Loess Plateau. Ecol Evol 10:12211–12223

    Article  PubMed  PubMed Central  Google Scholar 

  • WoliÅ„ska A, Stepniewska Z (2012) Dehydrogenase activity in the soil environment. In: Dehydrogenases. Intech Open, London

    Google Scholar 

  • Yang ST, El-Enshasy HA, Thongchul N (2013) Cellulases: characteristics, sources, production, and applications. In: Zhang X-Z, Yi-Heng PZ (eds) Bioprocessing technologies in biorefinery for sustainable production of fuels, chemicals, and polymers, 1st edn. Wiley, Hoboken, pp 131–146

    Chapter  Google Scholar 

  • Zhang NH, Xing-Dong G, Yu-Bao LI, Yong-Hong W, Hai-Tao MA, Di ZR, Yang S (2010) Pedogenic carbonate and soil dehydrogenase activity in response to soil organic matter in Artemisia Ordosica community. Pedosphere 20:229–235

    Article  CAS  Google Scholar 

  • Zhang T, Wan S, Kang Y, Feng H (2014) Urease activity and its relationships to soil physiochemical properties in a highly saline-sodic soil. J Soil Sci Plant Nutr 14(2):304–315

    Article  CAS  Google Scholar 

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Neemisha, Sharma, S. (2022). Soil Enzymes and Their Role in Nutrient Cycling. In: Giri, B., Kapoor, R., Wu, QS., Varma, A. (eds) Structure and Functions of Pedosphere. Springer, Singapore. https://doi.org/10.1007/978-981-16-8770-9_8

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