Skip to main content
SpringerLink
Log in

Diversity of Trigonella foenum graecum Microsymbionts in Morocco

  • Chapter
  • First Online:
Fenugreek

Abstract

Trigonella foenum graecum is a medicinal and culinary plant with varied health benefits. The improvement of its growth in weak soils depends on symbiotic soil bacteria called rhizobia which contribute to the autonomy of legumes towards nitrogen. In this work, we characterized 75 bacteria isolated from root nodules of Fenugreek grown in different soils of the Eastern Morocco with the aim to select some performant strains to use as inoculum for the improvement of the plant growth and yield in weak soils of Moroccan semi-arid areas. We found a great phenotypic diversity of fenugreek rhizobia and they were more tolerant to environmental stresses than the reference strains. The strain S3D has similar behavior as Ensifer meliloti strain USDA1002, whereas the other strains analyzed were different from the studied reference strains. There was no relationship between rep-PCR fingerprinting and phenotypic properties. The nearly 16S rRNA gene sequence of some representative strains showed they are closely related to the genus Ensifer with the highest similitude values (100%) with E. meliloti USDA1021. Sequencing of the symbiotic nodC gene from some representative strains revealed they were close to nodC sequences of different Ensifer meliloti strains. Host range tests revealed that all the fenugreek rhizobia nodulated several legumes from different Fabaceae tribes. In conclusion, the strains analyzed are very infective and may be used as inoculum in the arid areas of Morocco.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abdelmoumen, H., Filali-Maltouf, A., Neyra, M., Belabed, A., Missbah El-Idrissi, M., 1999. Effect of high salts concentrations on the growth of rhizobia and responses to added osmotica. J. Appl. Microbiol., 80, 889–898

    Google Scholar 

  • Ajabnoor M.A., Tilmisany A.K., 1989. Effect of Trigonella foenum graecum on blood glucose levels in normal and alloxan-diabetic mice. J. Ethnopharmacol., 26, 249–54

    Google Scholar 

  • Allen, O.N., Allen, E.K., 1981. The Leguminosae: a Source Book of Characteristics, Uses, and Nodulation, University of Wisconsin Press, Madison, WI

    Google Scholar 

  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., 1990. Basic local alignment search tool. J. Mol. Biol., 25, 403–410

    Google Scholar 

  • Azani, N., Babineau, M., Bailey, C. D., Banks, H., Barbosa, A. R., Pinto, R. B., et Candido, E., (2017). A new subfamily classification of the Leguminosae based on a taxonomically comprehensive phylogeny The Legume Phylogeny Working Group (LPWG). Taxon, 66(1), 44–77

    Google Scholar 

  • Benata H, Ourarhi M., Boukhatem N., Abdelmoumen H., Berrichi, R. Muresu, A., Squartini A. and M. Missbah El Idrissi. (2008) Diversity of bacteria that nodulate Prosopis juliflora in the eastern area of Morocco. Syst. Appl Microbiol. 31 (5), 378–386

    Google Scholar 

  • Beringer, J. E., 1974. Factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol., 84, 188–198

    Google Scholar 

  • Boivin C., Giraud E., 1999. Molecular symbiotic characterization of rhizobia: Toward a polyphasic approach using Nod factors and nod genes, in: Martínez-Romero E., Hernández G. (Eds.), Highlights of Nitrogen Fixation Research, Kluwer Academic/Plenum Publishers

    Google Scholar 

  • Bournaud C., Moulin L., Cnockaert M., de Faria S., Prin Y., Severac D. & Vandamme, P. (2017) Paraburkholderia piptadeniae sp. nov. and Paraburkholderia ribeironis sp. nov., two root-nodulating symbiotic species of Piptadenia gonoacantha in Brazil. International Journal of Systematic and Evolutionary Microbiology 67, 432–440

    Google Scholar 

  • Brenner, D.J., McWhorter, A.C., Knutson, J.K., Steigerwalt, A.G., 1982. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds, J. Clin. Microbiol., 5, 1133–1140

    Google Scholar 

  • Castro, I.V.; Fareleira, P. and Ferreira, E. (2016) - Nitrogen Fixing Symbioses in a Sustainable Agriculture. In: Hakeem, K.R.; Akhtar, M.S. & Abdullah, S.N.A. (Eds) - Plant, Soil and Microbes. Volume 1: Implications in Crop Science, p. 55–91

    Google Scholar 

  • Cooper, J.E. (2007). Early interactions between legumes and rhizobia: disclosing complexity in a molecular dialogue. J. Appl. Microbiol. 103 (5), 1355–1365

    Google Scholar 

  • Cubo, M.T., Buendia-Claveria A. M, Beringer and Ruiz-Sainz J.E., 1988. Melanin production by Rhizobium strains. Appl. Environ. Microbiol. 54, 1812–1817

    Google Scholar 

  • Dommergues Y., Duhoux E., Diem H.G., 1999. Les arbres fixateurs d’azote. Editions Espace 34, Montpellier

    Google Scholar 

  • Duke, J.A., 1981. Trigonella foenum-graecum L., In: Handbook of legumes of world economic importance. Plenum Press, New York

    Google Scholar 

  • Eardly, B., Elia, P., Brockwell, J., Golemboski, D., and van Berkum, P. (2017). Biogeography of a Novel Ensifer meliloti Clade Associated with the Australian Legume Trigonella suavissima. Appl Environ Microbiol, 83(10): e03446-16. doi: https://doi.org/10.1128/AEM.03446-16

  • Ernst, W. H. O., 1990. Mine vegetation in Europe, p. 21. In A. J. Shaw (ed.), Heavy metal tolerance in plants: evolutionary aspects. CRC Press, Boca Raton, Fla

    Google Scholar 

  • Foury, A., 1954. Les lÕgumineuses fourragÒres au Maroc. Les cahiers de la recherche agronomique. N° 3 - 5, pp 33–3 8. Eds. Service de la recherche agronomique, Rabat

    Google Scholar 

  • El Batanony, N.H., Castellano-Hinojosa, A., Correa-Galeote, D., Bedmar, E.J. 2015. The diversity of rhizobia nodulating the Medicago, Melilotus and Trigonella inoculation group in Egypt is marked by the dominance of two genetic types. Symbiosis, 67 (1–3), 3–10

    Google Scholar 

  • Gabay M. P., 2002. Galactogogues: medications that induce lactation. J. Hum. Lact., 18(3), 274–9

    Google Scholar 

  • Graham, P. H., Parker, C. A., 1964. Diagnostic features in the characterization of root nodule bacteria of legumes. Plant Soil, 20, 3 83–396

    Google Scholar 

  • Guerrouj K., Ruíz-Díez B., Chahboune R., RamÚrez-Bahena M-H., Abdelmoumen H., Quiñones M. A., Missbah El Idrissi M., VelÃzquez E., Fernández-Pascual M., Bedmar E. J., Peix A. 2013. Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma. Systematic and Applied Microbiology. 36: 218–223

    Google Scholar 

  • Kimura M., 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol.,6,111-120

    Google Scholar 

  • Kumar, H., Dubey, R.C., Maheshwari, D.K. 2018. Rhizobial genetic diversity in root nodules of Trigonella foenum-graecum cultivated in sub-Himalayan region of Uttarakhand. Biocatalysis and Agricultural Biotechnology, 16, 243–252

    Google Scholar 

  • Laguerre, G., vanBerkum, P., Amarger, N., Prevost, D. 1997. Genetic diversity of rhizobial symbionts isolated from legume species within the genera Astragalus, Oxytropis, and Onobrychis. Appl. Environ. Microbiol. 63(12), 4748–4758

    Google Scholar 

  • Lindström, K., Lehtomäki, S., 1988. Metabolic properties, maximum growth temperature and phase sensitivity of rhizobium sp. (Galega) compared with other fastgrowing rhizobia. FEMS Microbiol. Lett., 50, 277–287

    Google Scholar 

  • Mir, Z., Mir P.S., Acharya S.N., Zaman M.S. et al., 1998. Comparison of alfalfa and fenugreek silages supplemented with barley grain on performance of growing steers. Can. J. Anim. Sci., 78, 343–349

    Google Scholar 

  • Mishkinsky, J.S., Goldschmied, A., Joseph, B. Ahronson, Z., Sulman, F.G., 1977. Hypoglycaemic effect of Trigonella foenum graecum and Lupinus termis (leguminosae) seeds and their major alkaloids in alloxan-diabetic and normal rats. Pahlavi Med. J., 8, 14–25

    Google Scholar 

  • Missbah El Idrissi, M., Auajjar, N., Belabed, A., Dessaux, Y., Filali-Maltouf, A., 1996. Characterization of rhizobia isolated from carob tree Ceratonia siliqua, J. Appl. Bacteriol.,80: 165–173

    Google Scholar 

  • Mohammed, S. H., Smouni, A., Neyra, M., Kharchaf, D., Filali-Matouf, A. (2000). Phenotypic characteristics of root-nodulating bacteria isolated from Acacia spp. grown in Libya. Plant Soil. 224, 171–183

    Google Scholar 

  • Mousavi, S. A., Osterman, J., Wahlberg, N., Nesme, X., et al., 2014. Phylogeny of the Rhizobium–Allorhizobium–Agrobacterium clade supports the delineation of Neorhizobium gen. nov. Syst. Appl. Microbiol., 37, 208–215

    Google Scholar 

  • Munnevar, F., Wollum, A.G., 1981. Effect of high root temperature and Rhizobium strain on nodulation, nitrogen fixation and growth of soybeans. Soil Sci. A., 24, 1113–1120

    Google Scholar 

  • Ourarhi M., Abdelmoumen H., Guerrouj K., Benata H., Muresu R., Squartini A., Missbah El Idrissi M. 2010. Colutea arborescens is nodulated by diverse rhizobia in Eastern Morocco. Arch. Microbiol. 193: 115–24. doi: 10.1007/s00203-010-0650-0

    Google Scholar 

  • Robert, F.M., Molina, J.A., Schmidt, E.L., 1982. Properties of Rhizobium leguminosarum isolated from various regions of Morocco. Ann. Microbiol., /33(3), 46 1–70

    Google Scholar 

  • Sachiko, M., Setsuko, N., Shi, X., 2001. Expression of the cytoplasmic domain of NodC as an active form in drosophila S2 cells. J. Biosci. Bioeng., 9/, 25 1–255

    Google Scholar 

  • Saitou, N., Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425

    Google Scholar 

  • Sneath, P.H.A., Sokal, R.R., 1973. Numerical Taxonomy. The Principles and Practices of Numerical Classification. San Francisco: W. H. Freeman

    Google Scholar 

  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol. Biol. Evol., 30, 2725–2729

    Google Scholar 

  • Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res., 5, (24), 4876–4882

    Google Scholar 

  • Van de Sande, K., Bisseling, T., 1997. Signalling in symbiotic root nodule formation. Ass. Biochem., 32, 127–142

    Google Scholar 

  • Versalovic, J.T., Schneider, M., de Bruijn F.J., Lupski, J. R., 1994. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol. Cell. Biol. 5, 25–40

    Google Scholar 

  • Vincent, J.M., 1970. A manual for the practical study of root nodule bacteria, Blackwell Scientific publications, Oxford, pp. 164. IBP Handbook, N° 15

    Google Scholar 

  • Wdowiak-Wróbel S, Marek-Kozaczuk M, Kalita M, Karaś M, Wójcik M, Małek W. (2017) Diversity and plant growth promoting properties of rhizobia isolated from root nodules of Ononis arvensis. Antonie Van Leeuwenhoek. 110(8):1087–1103. doi: 10.1007/s10482-017-0883-x

    Google Scholar 

  • Zerhari, K., Aurag, J., Khbaya, B., Kharchaf, D., Filali-Maltouf, A., 2000. Phenotypic characteristics of rhizobia isolates nodulating Acacia species in the arid and Saharan regions of Morocco. Lett. Appl. Microbiol., 30(5), 351–357

    Google Scholar 

  • Zurdo-Piñeiro JL, García-Fraile P, Rivas R, Peix A, León-Barrios M, Willems A, Mateos PF, Martínez-Molina E, Velázquez E, van Berkum P. 2009. Rhizobia from Lanzarote, the Canary Islands, that nodulate Phaseolus vulgaris have characteristics in common with Sinorhizobium meliloti isolates from mainland Spain. Appl Environ Microbiol, 75(8):2354–9. doi: https://doi.org/10.1128/AEM.02811-08

Download references

Acknowledgement

The authors want to thank all the persons who contributed to this work.

Author information

Authors and Affiliations

  1. Centre de Biotechnologies Végétales et Microbiennes, Faculty of Sciences, Mohammed V University in Rabat, Rabat, Morocco

    Mustapha Missbah El Idrissi & Hanaa Abdelmoumen

Authors
  1. Mustapha Missbah El Idrissi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hanaa Abdelmoumen
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    M. Naeem

  2. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Tariq Aftab

  3. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    M. Masroor A. Khan

Rights and permissions

Reprints and permissions

Copyright information

© 2021 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

El Idrissi, M.M., Abdelmoumen, H. (2021). Diversity of Trigonella foenum graecum Microsymbionts in Morocco. In: Naeem, M., Aftab, T., Khan, M.M.A. (eds) Fenugreek. Springer, Singapore. https://doi.org/10.1007/978-981-16-1197-1_13

Download citation

Publish with us

Policies and ethics

Search

Navigation