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Meta-topolin and Related Cytokinins as a Solution to Some In Vitro Problems

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Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture

Abstract

Plant micropropagation does not always run smoothly. With a number of plants, problems such as hyperhydricity, shoot-tip necrosis, early senescence, and poor rooting are bottlenecks. These difficulties prevent the large-scale exploitation of elite genotypes of food crops, ornamental plants and fruit, forest, and timber trees. In this chapter the current evidence is summarized that shows that important in vitro problems can be alleviated using the right type and concentration of meta-topolin or a member of its expanding group of derived analogues.

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References

  • Ahmad A, Anis M (2019) Meta-topolin improves in vitro morphogenesis, rhizogenesis and biochemical analysis in Pterocarpus marsupium Roxb.: a potential drug-yielding tree. J Plant Growth Regul 38(3):1007–1016

    Google Scholar 

  • Amoo SO, Aremu AO, Moyo M et al (2014) Physiological effects of a novel aromatic cytokinin analogue in micropropagated Aloe arborescens and Harpagophytum procumbens. Plant Cell Tissue Organ Cult 116(1):17–26

    Google Scholar 

  • Aremu AO, Bairu MW, Szüčová L et al (2012) Assessment of the role of meta-topolins on in vitro produced phenolics and acclimatization competence of micropropagated ‘Williams’ banana. Acta Physiol Plant 34(6):2265–2273

    Google Scholar 

  • Bairu MW, Stirk WA, Dolezal K, Van Staden J (2007) Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell Tissue Organ Cult 90(1):15–23

    Google Scholar 

  • Bairu MW, Stirk WA, Van Staden J (2009) Factors contributing to in vitro shoot-tip necrosis and their physiological interactions. Plant Cell Tissue Organ Cult 98(3):239–248

    Google Scholar 

  • Bairu MW, Novák O, Doležal K, Van Staden J. (2011) Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments. Plant Growth Regul 63(2):105–114

    Google Scholar 

  • Behera S, Kar SK, Rout KK et al (2019) Assessment of genetic and biochemical fidelity of field-established Hedychium coronarium J. Koenig regenerated from axenic cotyledonary node on meta-topolin supplemented medium. Indus Crop Prod 134:206–215

    Google Scholar 

  • Bogaert I, Van Cauter S, Prinsen E et al (2006) New aromatic cytokinin can make the difference. Acta Hortic 725:265–270

    Google Scholar 

  • Dobránszki J, Magyar-Tábori K, Jámbor-Benczúr E, et al. (2002) Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves. Acta Agron Hung 50(2):117–126

    Google Scholar 

  • Doležal K, Popa I, Kryštof V et al (2006) Preparation and biological activity of 6-benzylaminopurine derivatives in plants and human cancer cells. Bioorg Med Chem 14:875–884

    Google Scholar 

  • Gentile A, Frattarelli A, Nota P et al (2017) The aromatic cytokinin meta-topolin promotes in vitro propagation, shoot quality and micrografting in Corylus colurna L. Plant Cell Tissue Organ Cult 128(3):693–703

    Google Scholar 

  • Horgan R, Hewett EW, Purse JG, Wareing PF (1973) A new cytokinin from Populus x robusta. Tetrahedron 30:2827–2828

    Google Scholar 

  • Ivanova M, van Staden J (2008) Effect of ammonium ions and cytokinins on hyperhydricity and multiplication rate of in vitro regenerated shoots of Aloe polyphylla. Plant Cell Tissue Organ Cult 92(2):227–231

    Google Scholar 

  • Kadota M, Niimi Y (2003) Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tissue Organ Cult 72(3):261–265

    Google Scholar 

  • Lata H, Chandra S, Techen N et al (2016) In vitro mass propagation of Cannabis sativa L.: a protocol refinement using novel aromatic cytokinin meta-topolin and the assessment of eco-physiological, biochemical and genetic fidelity of micropropagated plants. J Appl Res Med Arom Plants 3(1):18–26

    Google Scholar 

  • Mutui TM, Mibus H, Serek M (2012) Effect of meta-topolin on leaf senescence and rooting in Pelargonium× hortorum cuttings. Posthar Biol Technol 63(1):107–110

    Google Scholar 

  • Naaz A, Hussain SA, Anis M, Alatar AA (2019) Meta-topolin improved micropropagation in Syzygium cumini and acclimatization to ex vitro conditions. Biol Plant 63(1):174–182

    Google Scholar 

  • Naidoo D, Aremu AO, Van Staden J, Finnie JF (2017) In vitro plant regeneration and alleviation of physiological disorders in Scadoxus puniceus. S Afr J Bot 109:316–322

    Google Scholar 

  • Palavan-Ünsal N, Çağ S, Çetin E et al (2002) Retardation of senescence by meta-topolin in wheat leaves. J Cell Mol Biol 1:101–108

    Google Scholar 

  • Poisson AS, Berthelot P, Le BC a (2016) A droplet-vitrification protocol enabled cryopreservation of doubled haploid explants of Malus x domestica Borkh. ‘Golden delicious’. Sci Hortic 209:187–191

    Google Scholar 

  • Rojas-Martínez L, Visser RG, De Klerk GJ (2010) The hyperhydricity syndrome: waterlogging of plant tissues as a major cause. Propag Ornam Plants 10(4):169–175

    Google Scholar 

  • Saeiahagh H, Mousavi M, Wiedow C et al (2019) Effect of cytokinins and sucrose concentration on the efficiency of micropropagation of ‘Zes006’ Actinidia chinensis var. chinensis, a red-fleshed kiwifruit cultivar. Plant Cell Tissue Organ Cult 138:1–10

    Google Scholar 

  • Strnad M, Peters W, Hanuš J, Beck E (1994) Ortho-topolin-9-glucoside, an aromatic cytokinin from Populusxcanadensis cv Robusta leaves. Phytochemistry 37(4):1059–1062

    Google Scholar 

  • Strnad M, Hanuš J, Vaněk T et al (1997) Meta-topolin, a highly active aromatic cytokinin from poplar leaves (Populus×canadensis Moench., cv. Robusta). Phytochemistry 45(2):213–218

    Google Scholar 

  • Szüčová L, Spíchal L, Doležal K et al (2009) Synthesis, characterization and biological activity of ring-substituted 6-benzylamino-9-tetrahydropyran-2-yl purine derivatives. Bioorg Med Chem 17(5):1938–1947

    Google Scholar 

  • Tarkowská D, Doležal K, Tarkowski P et al (2003) Identification of new aromatic cytokinins in Arabidopsis thaliana and Populus× canadensis leaves by LC-(+) ESI-MS and capillary liquid chromatography/frit–fast atom bombardment mass spectrometry. Physiol Plant 117(4):579–590

    Google Scholar 

  • Valero-Aracama C, Kane ME, Wilson SB et al (2010) Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult-and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes. Plant Growth Regul 60(1):43

    Google Scholar 

  • Van der Westhuizen A (2014) The use of meta-topolin as an alternative cytokinin in the tissue culture of Eucalyptus species. Acta Hortic 1055(1055):25–28

    Google Scholar 

  • Van Staden J, Cook EL, Noodén LD (1988) Cytokinins and senescence. In: Noodén LD, Leopold AC (eds) Senescence and aging in plants. Academic, San Diego, pp 281–328

    Google Scholar 

  • Werbrouck SP, van der Jeugt B, Dewitte W et al (1995) The metabolism of benzyladenine in Spathiphyllum floribundum ‘Schott Petite’ in relation to acclimatisation problems. Plant Cell Rep 14(10):662–665

    Google Scholar 

  • Werbrouck SP, Strnad M, Van Onckelen HA et al (1996) Meta-topolin, an alternative to benzyladenine in tissue culture? Physiol Plant 98(2):291–297

    Google Scholar 

  • Wojtania A (2010) Effect of meta-topolin on in vitro propagation of Pelargonium x hortorum and Pelargonium x hederaefolium cultivars. Acta Soc Bot Pol 79:101–106

    Google Scholar 

  • Zwack PJ, Rashotte AM (2013) Cytokinin inhibition of leaf senescence. Plant Signal Behav 8(7):e24737

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Applied In Vitro Plant Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium

    Stefaan P. O. Werbrouck

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  1. Stefaan P. O. Werbrouck
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Correspondence to Stefaan P. O. Werbrouck .

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Editors and Affiliations

  1. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Naseem Ahmad

  2. Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences and Palacký University, Olomouc, Czech Republic

    Miroslav Strnad

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Werbrouck, S.P.O. (2021). Meta-topolin and Related Cytokinins as a Solution to Some In Vitro Problems. In: Ahmad, N., Strnad, M. (eds) Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture. Springer, Singapore. https://doi.org/10.1007/978-981-15-9046-7_9

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