Abstract
Presently, nanoparticles (NPs) technology is a booming business marked by a significantly fast growth rate that covers a wide range of industries. NPs demand increases and they have a potential market value. The nanotoxicity sector has grown significantly for the last 10–15 years, which will pose serious problems in the coming future. Nano-toxicology is an innovative area of toxicological study that assesses the toxicological assets of NPs to decide whether they constitute a risk or an ecological problem and to what degree. To assess the different NPs toxicity, numerous nanotoxicological studies were piloted using different methods. The vital mechanisms of nanomaterial toxicity were recently studied especially in aquatic wildlife. In recent years, nanoparticle toxicity evaluation amplified exponentially by using zebrafish as an animal prototypical system. Zebrafish has been tested as an established model system for experimental biological study and are evolving as a solid nanotoxicity prototype which is progressively used as an in vivo model. It is principally used as a platform for rapid testing and assortment of molecules in the object or phenotype techniques. It offers a number of advantages over other living prototypes by offering prospects to speedily screen nanoparticulate medicines beneath in-vivo environments, also an economical way to link the present gap among in vitro and vertebrate studies. Many researchers have summarized experimental parameters critically used by zebrafish as an animal model for biomedical tests such as sample size, organ, and type wild against transgenic lines. Current chapter will discuss considerable factors of experimentation, advantages, and usage of zebrafish in nanomedicine; different methods of evaluating the nanotoxicity such as hatching exploration; malformation of embryos and organs of development; genetically modified zebrafish by means of living biosensor; disturbance in the endocrine system, skin, and gill; reproductive toxicity; genotoxicity; neurotoxicity; immune-toxicity; and behavioral analysis. Furthermore, it will also discuss an overview of studies about investigation of the toxicity of silver, carbon nanotube, metal oxide, and quantum dots nanoparticles using zebrafish. At the end, future lookouts of zebrafish model are discussed. It is projected that this chapter will update study directed at emerging biocompatible nanoparticulates for a choice of uses and toxicity investigation.
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References
Ali S, Champagne DL, Spaink HP, Richardson MK (2011) Zebrafish embryos and larvae: a new generation of disease models and drug screens. Birth Defects Res C Embryo Today 93:115–133
Ali-Boucetta H, Al-Jamal KT, Kostarelos K (2011) Cytotoxic assessment of carbon nanotube interaction with cell cultures. Methods Mol Biol 726:299–312
Asharani PV, Lian Wu Y, Gong Z, Valiyaveettil S (2008a) Toxicity of silver nanoparticles in zebrafish models. Nanotechnol 19:255102
Asharani PV, Serina NG, Nurmawati MH, Wu YL, Gong Z, Valiyaveettil S (2008b) Impact of multi-walled carbon nanotubes on aquatic species. J Nanosci Nanotechnol 8:3603–3609
Bar-Ilan O, Albrecht RM, Fako VE, Furgeson DY (2009) Toxicity assessments of multisized gold and silver nanoparticles in zebrafish embryos. Small 5:1897–1910
Beasley A, Elrod-Erickson M, Otter RR (2012) Consistency of morphological endpoints used to assess developmental timing in zebrafish (Danio rerio) across a temperature gradient. Reprod Toxicol 34:561–567
Beliaeva NF, Kashirtseva VN, Medvedeva NV, Khudoklinova I, Ipatova OM, Archakov AI (2010) Zebrafish as a model organism for biomedical studies. Biomed Khim 56:120–131
Belyaeva NF, Kashirtseva VN, Medvedeva NV, Khudoklinova YY, Ipatova OM, Archakov AI (2009) Zebrafish as a model system for biomedical studies: review. Biochem (moscow) Suppl Ser B Biomed Chem 3(4):343–350
Bohnsack JP, Assemi S, Miller JD, Furgeson DY (2012) The primacy of physicochemical characterization of nanomaterials for reliable toxicity assessment: a review of the zebra fish nanotoxicology model. In: Reineke J (ed) Nanotoxicity: methods and protocols, methods in molecular biology, vol 926. Springer Science Business Media, LLC, New York, pp 261–316
Bolognesi C (2003) Genotoxicity of pesticides: a review of human biomonitoring studies. Mutat Res 543:251–272
Braunbeck T, Bottcher M, Hollert H, Kosmehl T, Lammer E, Leist E, Rudolf M, Seitz N (2005) Towards an alternative for the acute fish LC50 test in chemical assessment: the fish embryo toxicity test goes multi-species—An Update. ALTEX 22(2/05):87–102
Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann MC (2005) In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol Sci 88:412–419
Busquet F, Nagel R, Landenberg FV, Mueller SO, Huebler N, Broschard TH (2008) Development of a new screening assay to identify proteratogenic substances using zebrafish Danio rerio embryo combined with an exogenous mammalian metabolic activation system (mDarT). Toxicol Sci 104(1):177–188
Cambier S, Gonzalez P, Durrieu G, Bourdineaud JP (2010) Cadmium-induced genotoxicity in zebrafish at environmentally relevant doses. Ecotoxicol Environ Saf 73:312–319
Campbell F, Bos FL, Sieber S, Arias-Alpizar G, Koch BE, Huwyler J, Kros A, Bussmann J (2018) Directing nanoparticle bio distribution through evasion and exploitation of stab2-dependent nanoparticle uptake. ACS Nano 12:2138–2150
Cela P, Vesela B, Matalova E, Vecera Z, Buchtova M (2014) Embryonic toxicity of nanoparticles. Cells Tissues Organs 199:1–23
Chakraborty C, Agoramoorthy G (2010) Why zebrafish? Riv Biol 103:25–27
Chakraborty C, Hsu CH, Wen ZH, Lin CS, Agoramoorthy G (2009) Zebrafish: a complete animal model for in vivo drug discovery and development. Curr Drug Metab 10:116–124
Chakraborty C, Sharma AR, Sharma G, Lee SS (2016) Zebrafish: a complete animal model to enumerate the nanoparticle toxicity. J Nanobiotechnol 14:65
Chen TH, Lin CY, Tseng MC (2011) Behavioral effects of titanium dioxide nanoparticles on larval zebrafish (Danio rerio). Mar Pollut Bull 63:303–308
Dai Q, Bertleff-Zieschang N, Braunger JA, Bjornmalm M, Cortez-Jugo C, Caruso F (2018) Particle targeting in complex biological media. Adv Healthc Mater 7:1700575
Daroczi B, Kari G, McAleer MF, Wolf JC, Rodeck U, Dicker AP (2006) In-vivo radioprotection by the fullerene nanoparticle DF-1 as assessed in a zebrafish model. Clin Cancer Res 12:7086–7091
Dedeh A, Ciutat A, Treguer-Delapierre M, Bourdineaud JP (2015) Impact of gold nanoparticles on zebrafish exposed to a spiked sediment. Nanotoxicol 9:71–80
Delorme-Axford E, Guimaraes RS, Reggiori F, Klionsky DJ (2015) The yeast Saccharomyces cerevisiae: an overview of methods to study autophagy progression. Methods 75:3–12
Di Gioacchino M, Petrarca C, Lazzarin F, Di Giampaolo L, Sabbioni E, Boscolo P, Mariani-Costantini R, Bernardini G (2011) Immunotoxicity of nanoparticles. Int J Immunopathol Pharmacol 24:65S–71S
Embry MR, Belanger SE, Braunbeck TA, Galay-Burgos M, Halder M, Hinton DE, Leonard MA, Lillicrap A, Norberg-King T, Whale G (2010) The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and scientific research. Aquat Toxicol 97(2):79–87
Faklaris O, Joshi V, Irinopoulou T, Tauc P, Sennour M, Girard H et al (2009) Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells. ACS Nano 3:3955–3962
Fako VE, Furgeson DY (2009) Zebrafish as a correlative and predictive model for assessing biomaterial nanotoxicity. Adv Drug Deliv Rev 61:478–486
Fernandez-Garcia M, Rodriguez JA (2007) Metal oxide nanoparticles. Encyclopedia inorganic bioinorganic chemistry. Wiley, New York, NY
Foriel S, Willems P, Smeitink J, Schenck A, Beyrath J (2015) Mitochondrial diseases: Drosophila melanogaster as a model to evaluate potential therapeutics. Int J Biochem Cell Biol 63:60–65
Franke ME, Koplin TJ, Simon U (2006) Metal and metal oxide nanoparticles in chemiresistors: does the nanoscale matter? Small 2:36–50
Gad SC (2014) Animal models in toxicology. CRC, London, p 983
Gambardella C, Gallus L, Gatti AM, Faimali M, Carbone S, Antisari LV (2014) Toxicity and transfer of metal oxide nanoparticles from microalgae to sea urchin larvae. Chem Ecol 30:308–316
Geffroy B, Ladhar C, Cambier S, Treguer-Delapierre M, Brethes D, Bourdineaud JP (2012) Impact of dietary gold nanoparticles in zebrafish at very low contamination pressure: the role of size, concentration and exposure time. Nanotoxicol 6:144–160
George S, Lin S, Ji Z, Thomas CR, Li L, Mecklenburg M, Meng H, Wang X, Zhang H, Xia T et al (2011) Surface defects on plate-shaped silver nanoparticles contribute to its hazard potential in a fish gill cell line and zebrafish embryos. ACS Nano 6:3745–3759
Gonalez-Moragas L, Roig A, Laromaine A (2015) C. elegans as a tool for in vivo nanoparticle assessment. Adv Colloid Interf Sci 219:10–26
Griffitt RJ, Weil R, Hyndman KA, Denslow ND, Powers K, Taylor D, Barber DS (2007) Exposure to copper nanoparticles causes gill injury and acute lethality in zebrafish (Danio rerio). Environ Sci Technol 41:8178–8186
Gustafson HH, Holt-Casper D, Grainger DW, Ghandehari H (2015) Nanoparticle uptake: the phagocyte problem. Nano Today 10:487–510
Haque E, Ward AC (2018) Zebrafish as a model to evaluate nanoparticle toxicity. Nano 8(561):1–18
Hill AJ, Teraoka H, Heideman W, Peterson RE (2005) Zebrafish as a model vertebrate for investigating chemical toxicity: review. Toxicol Sci 86(1):6–19
Hofmann D, Tenzer S, Bannwarth MB, Messerschmidt C, Glaser SF, Schild H, Landfester K, Mailander V (2014) Mass spectrometry and imaging analysis of nanoparticle-containing vesicles provide a mechanistic insight into cellular trafficking. ACS Nano 8:10077–10088
Hsu CH, Wen ZH, Lin CS, Chakraborty C (2007) The zebrafish model: use in studying cellular mechanisms for a spectrum of clinical disease entities. Curr Neurovasc Res 4:111–120
Huang Y, Zhang J, Han X, Huang T (2014) The use of zebrafish (Danio rerio) behavioral responses in identifying sublethal exposures to deltamethrin. Int J Environ Res Public Health 11:3650–3660
Hung KW, Suen MF, Chen YF, Cai HB, Mo ZX, Yung KK (2012) Detection of water toxicity using cytochrome P450 transgenic zebrafish as live biosensor: for polychlorinated biphenyls toxicity. Biosens Bioelectron 31:548–553
Igartua DE, Azcona PL, Martinez CS, Alonso SV, Lassalle VL, Prieto MJ (2018) Folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on zebrafish embryo and larvae. Toxicol Appl Pharmacol 358:23–34
Iguchi Y, Michiue H, Kitamatsu M, Hayashi Y, Takenaka F, Nishiki T, Matsui H (2015) Tumor-specific delivery of BSH-3R for boron neutron capture therapy and positron emission tomography imaging in a mouse brain tumor model. Biomaterials 56:10–17
Jang GH, Hwang MP, Kim SY, Jang HS, Lee KH (2014) A systematic in-vivo toxicity evaluation of nanophosphor particles via zebrafish models. Biomaterials 35:440–449
Jin Y, Zheng S, Fu Z (2011) Embryonic exposure to cypermethrin induces apoptosis and immunotoxicity in zebrafish (Danio rerio). Fish Shellfish Immunol 30:1049–1054
Kalishwaralal K, Jeyabharathi S, Sundar K, Muthukumaran A (2016) A novel one-pot green synthesis of selenium nanoparticles and evaluation of its toxicity in zebrafish embryos. Artif Cells Nanomed Biotechnol 44(2):471–477
Kannan RR, JerleyAJA RM, Prakash VSG (2011) Antimicrobial silver nanoparticle induces organ deformities in the developing zebrafish (Danio rerio) embryos. J Biomed Sci Eng 4:248–254
Kari G, Rodeck U, Dicker AP (2007) Zebrafish: an emerging model system for human disease and drug discovery. Discovery 82(1):70–80
Karlsson J, von Hofsten J, Olsson PE (2001) Generating transparent zebrafish: a refined method to improve detection of gene expression during embryonic development. Mar Biotechnol 3:522–527
Keller JM, Escara-Wilke JF, Keller ET (2008) Heat stress-induced heat shock protein 70 expression is dependent on ERK activation in zebrafish (Danio rerio) cells. Comp Biochem Physiol A Mol Integr Physiol 150:307–314
Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF (1995) Stages of embryonic development of the zebrafish. Dev Dyn 203:253–310
King Heiden TC, Dengler E, Kao WJ, Heideman W, Peterson RE (2007) Developmental toxicity of low generation PAMAM dendrimers in zebrafish. Toxicol Appl Pharmacol 225:70–79
King-Heiden TC, Wiecinski PN, Mangham AN et al (2009) Quantum dot nanotoxicity assessment using the zebrafish embryo. Environ Sci Technol 43(5):1605–1611
Kokel D, Bryan J, Laggner C, White R, Cheung CY, Mateus R, Healey D, Kim S, Werdich AA, Haggarty SJ et al (2010) Rapid behavior-based identification of neuroactive small molecules in the zebrafish. Nat Chem Biol 6:231–237
KovriZnych JA, Sotnikova R, Zeljenkov A, Rollerov A, Szabova E (2014) Long-term (30 days) toxicity of NiO nanoparticles for adult zebrafish Danio rerio. Interdiscip Toxicol 7(1):23–26
Lankveld DP, Van Loveren H, Baken KA, Vandebriel RJ (2010) In-vitro testing for direct immunotoxicity: state of the art. Methods Mol Biol 598:401–423
Lee HC, Lu PN, Huang HL, Chu C, Li HP, Tsai HJ (2014) Zebrafish transgenic line huORFZ is an effective living bioindicator for detecting environmental toxicants. PLoS One 9:e90160
Lee KY, Jang GH, Byun CH, Jeun M, Peter-Searson C, Lee KH (2017) Zebrafish models for functional and toxicological screening of nanoscale drug delivery systems: promoting preclinical applications. Biosci Rep 37:1–13
Liegertova M, Wrobel D, Herma R, Müllerova M et al (2018) Evaluation of toxicological and teratogenic effects of carbosilane glucose glycodendrimers in zebrafish embryos and model rodent cell lines. Nanotoxicol 12(8):797–818
Lin S, Zhao Y, Nel AE, Lin S (2013) Zebrafish: an in-vivo model for nano EHS studies. Small 9(0):1608–1618
Liu Z, Tabakman S, Welsher K, Dai H (2009) Carbon nanotubes in biology and medicine: in vitro and in vivo detection imaging and drug delivery. Nano Res 2:85–120
MacPhail RC, Hunter DL, Irons TD, Padilla S (2011) Locomotion and behavioral toxicity in larval zebrafish: background, methods, and data. In: McGrath P (ed) Zebrafish methods assess drug safety and toxicity. Wiley, Hoboken, NJ, pp 151–164
MacRae CA, Peterson RT (2015) Zebrafish as tools for drug discovery. Nat Rev Drug Discov 14:721–731
Madani SY, Mandel A, Seifalian AM (2013) A concise review of carbon nanotube’s toxicology. Nano Rev 4:21521
Maynard AD, Warheit DB, Philbert MA (2011) The new toxicology of sophisticated materials: nanotoxicology and beyond. Toxicol Sci 120(Suppl 1):S109–S129
Mitragotri S, Lammers T, Bae YH, Schwendeman S et al (2017) Drug delivery research for the future: expanding the nano horizons and beyond. J Control Release 246:183–184
Morimoto Y, Kobayashi N, Shinohara N, Myojo T, Tanaka I, Nakanishi J (2010) Hazard assessments of manufactured nanomaterials. J Occup Health 52:325–334
OECD (2013) Guideline for the testing of chemicals. Fish Embryo Toxicity (FET), Paris, France
Ong KJ, Zhao X, Thistle ME, Maccormack TJ, Clark RJ, Ma G, Martinez- Rubi Y, Simard B, Loo JS, Veinot JG, Goss GG (2014) Mechanistic insights into the effect of nanoparticles on zebrafish hatch. Nanotoxicology 8:295–304
Paterson G, Ataria JM, Hoque ME, Burns DC, Metcalfe CD (2011) The toxicity of titanium dioxide nanopowder to early life stages of the Japanese medaka (Oryzias latipes). Chemosphere 82:1002–1009
Paunovska K, Sago CD, Monaco C, Hudson WH, Castro MG et al (2018) A direct comparison of in vitro and in vivo nucleic acid delivery mediated by hundreds of nanoparticles reveals a weak correlation. Nano Lett 18:2148–2157
Pecoraro R, D’Angelo D, Filice S, Scalese S, Capparucci F, Marino F, Iaria C, Guerriero G, Tibullo D, Scalisi EM, Salvaggio A, Nicotera I, Brundo MV (2017a) Toxicity evaluation of grapheme oxide and titania loaded nafion membranes in zebrafish. Front Physiol 8:1039
Pecoraro R, Salvaggio A, Marino F, Caro GD, Capparucci F, Lombardo BM, Messina G, Scalisi EM, Tummino M, Loreto F, D’Amante G, Avola R, Tibullo D, Brundo MV (2017b) Metallic nano-composite toxicity evaluation by zebrafish embryo toxicity test with identification of specific exposure biomarkers. Curr Protoc Toxicol 74:1.14.1–1.14.13
Rabergh CM, Airaksinen S, Soitamo A, Bjorklund HV, Johansson T, Nikinmaa M, Sistonen L (2000) Tissue-specific expression of zebrafish (Danio rerio) heat shock factor 1 mRNAs in response to heat stress. J Exp Biol 203:1817–1824
Rennekamp AJ, Peterson RT (2015) 15 years of zebrafish chemical screening. Curr Opin Chem Biol 24:58–70
Riccio EK, Pratt-Riccio LR, Bianco-Junior C, Sanchez V, Totino PR, Carvalho LJ, Daniel-Ribeiro CT (2015) Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research. Malar J 14:166
Roper C, Tanguay RL (2018) Chapter 12. Zebrafish as a model for developmental biology and toxicology. In: Handbook of developmental neurotoxicology. Elsevier, London, pp 143–151
Samaee SM, Rabbani S, Jovanovic B, Mohajeri-Tehrani MR, Haghpanah V (2015) Efficacy of the hatching event in assessing the embryo toxicity of the nano-sized TiO(2) particles in zebrafish: a comparison between two different classes of hatching-derived variables. Ecotoxicol Environ Saf 116:121–128
Sassen WA, Koster RW (2015) A molecular toolbox for genetic manipulation of zebrafish. Adv Genomics Genet 5:151–163
Seaton A, Tran L, Aitken R, Donaldson K (2010) Nanoparticles, human health hazard and regulation. J R Soc Interface 7(Suppl 1):S119–S129
Sheng L, Wang L, Su M, Zhao X, Hu R, Yu X, Hong J, Liu D, Xu B, Zhu Y et al (2014) Mechanism of TiO2 nanoparticle-induced neurotoxicity in zebrafish (Danio rerio). Environ Toxicol 31:163–175
Sieber S, Grossen P, Detampel P, Siegfried S, Witzigmann D, Huwyler J (2017) Zebrafish as an early stage screening tool to study the systemic circulation of nanoparticulate drug delivery systems in-vivo. J Control Release 264:180–191
Sieber S, Grossen P, Bussmann P, Campbell F, Kros A, Witzigmann D, Huwyler J (2019) Zebrafish as a preclinical in vivo screening model for nanomedicines. Adv Drug Deliv Rev 151–152:152–168
Strahle U, Scholz S, Geisler R, Greiner P, Hollert H, Rastegar S, Schumacher A, Selderslaghs I, Weiss C, Witters H, Braunbeck T (2012) Zebrafish embryos as an alternative to animal experiments—a commentary on the definition of the onset of protected life stages in animal welfare regulations. Reprod Toxicol 33:128–132
Sun YP, Cheng SH (2009) Acute and long-term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio). Toxicol Appl Pharmacol 235:216–225
Thanh NTK, Green LAW (2010) Functionalization of nanoparticles for biomedical applications. Nano Today 5:213–230
Thomas J, Vijayakumar S, Thanigaivel S, Mukherjee A, Chandrasekaran N (2014) Toxicity of magnesium oxide nano particles in two fresh water fishes tilapia (Oreochromis mossambicus) and zebra fish (Danio rerio). Int J Pharm Sci 6(2):487–490
Truong L, Saili KS, Miller JM, Hutchison JE, Tanguay RL (2012) Persistent adult zebrafish behavioral deficits results from acute embryonic exposure to gold nanoparticles. Comp Biochem Physiol C Toxicol Pharmacol 155:269–274
Tu W, Niu L, Liu W, Xu C (2013) Embryonic exposure to butachlor in zebrafish (Danio rerio): endocrine disruption, developmental toxicity and immunotoxicity. Ecotoxicol Environ Saf 89:189–195
Vargas A, Zeisser-Labouebe M, Lange N, Gurny R, Delie FV (2007) The chick embryo and its chorioallantoic membrane (CAM) for the in vivo evaluation of drug delivery systems. Adv Drug Deliv Rev 59:1162–1176
Varshney GK, Lu J, Gildea DE, Huang H, Pei W, Yang Z, Huang SC, Schoenfeld D, Pho NH, Casero D et al (2013) A large-scale zebrafish gene knockout resource for the genome-wide study of gene function. Genome Res 23:727–735
Villamizar N, Ribas L, Piferrer F, Vera LM, Sanchez-Vazquez FJ (2012) Impact of daily thermocycles on hatching rhythms, larval performance and sex differentiation of zebrafish. PLoS One 7:e52153
Wang J, Zhu X, Zhang X, Zhao Z, Liu H, George R, Wilson-Rawls J, Chang Y, Chen Y (2011) Disruption of zebrafish (Danio rerio) reproduction upon chronic exposure to TiO(2) nanoparticles. Chemosphere 83:461–467
Weiss C, Diabate S (2011) A special issue on nanotoxicology. Arch Toxicol 85:705–706
Wicki A, Witzigmann D, Balasubramanian V, Huwyler J (2015) Nanomedicine in cancer therapy: challenges, opportunities, and clinical applications. J Control Release 200:138–157
Witzigmann D, Hak S, Van der Meel R (2018) Translating nanomedicines: thinking beyond materials? A young investigator’s reply to ‘the novelty bubble’. J Control Release 290:138–140
Xin Q, Rotchell JM, Cheng J, Yi J, Zhang Q (2015) Silver nanoparticles affect the neural development of zebrafish embryos. J Appl Toxicol 35:1481–1492
Xu L, Liu Y, Chen Z, Li W, Wang L, Wu X, Ji Y, Zhao Y, Ma L, Shao Y, Chen C (2012a) Surface-engineered gold nanorods: promising DNA vaccine adjuvant for HIV-1 treatment. Nano Lett 12:2003–2012
Xu Z, Zhang YL, Song C, Wu LL, Gao HW (2012b) Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development. PLoS One 7(4):e32818
Xu H, Dong X, Zhang Z, Yang M, Wu X, Liu H, Lao Q, Li C (2015) Assessment of immunotoxicity of dibutyl phthalate using live zebrafish embryos. Fish Shellfish Immunol 45:286–292
Xu J, Zhang Q, Li X, Zhan S, Wang L, Chen D (2017) The effects of copper oxide nanoparticles on dorsoventral patterning, convergent extension, and neural and cardiac development of zebrafish. Aquat Toxicol 188:130–137
Zhang Q, Kopp M, Babiak I, Fernandes JMO (2018) Low incubation temperature during early development negatively affects survival and related innate immune processes in zebrafish larvae exposed to lipopolysaccharide. Sci Rep 8:4142
Zhao X, Wang S, Wub Y, Youa H, Lina LV (2013) Acute ZnO nanoparticles exposure induces developmental toxicity, oxidative stress and DNA damage in embryo-larval zebrafish. Aquat Toxicol 136–137:49–59
Zhuang S, Zhang Z, Zhang W, Bao L, Xu C, Zhang H (2015) Enantioselective developmental toxicity and immunotoxicity of pyraclofos toward zebrafish (Danio rerio). Aquat Toxicol 159:119–126
Zon DLI, Peterson RT (2005) In-vivo drug discovery in the zebrafish. Nat Rev Drug Discov 4:35–44
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Jagdale, S.C., Hude, R.U., Chabukswar, A.R. (2020). Zebrafish: A Laboratory Model to Evaluate Nanoparticle Toxicity. In: Siddhardha, B., Dyavaiah, M., Kasinathan, K. (eds) Model Organisms to Study Biological Activities and Toxicity of Nanoparticles. Springer, Singapore. https://doi.org/10.1007/978-981-15-1702-0_18
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