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Architecture of the Saccharomyces cerevisiae Replisome

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DNA Replication

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1042))

Abstract

Eukaryotic replication proteins are highly conserved, and thus study of Saccharomyces cerevisiae replication can inform about this central process in higher eukaryotes including humans. The S. cerevisiae replisome is a large and dynamic assembly comprised of ~50 proteins. The core of the replisome is composed of 31 different proteins including the 11-subunit CMG helicase; RFC clamp loader pentamer; PCNA clamp; the heteroligomeric DNA polymerases ε, δ, and α-primase; and the RPA heterotrimeric single strand binding protein. Many additional protein factors either travel with or transiently associate with these replisome proteins at particular times during replication. In this chapter, we summarize several recent structural studies on the S. cerevisiae replisome and its subassemblies using single particle electron microscopy and X-ray crystallography. These recent structural studies have outlined the overall architecture of a core replisome subassembly and shed new light on the mechanism of eukaryotic replication.

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Authors and Affiliations

  1. Cryo-EM Structural Biology Laboratory, Van Andel Research Institute, Grand Rapids, MI, USA

    Lin Bai, Zuanning Yuan, Jingchuan Sun & Huilin Li

  2. Biochemistry and Structural Biology Graduate Program, Stony Brook University, Stony Brook, NY, USA

    Zuanning Yuan & Huilin Li

  3. Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA

    Roxana Georgescu & Michael E. O’Donnell

Authors
  1. Lin Bai
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  2. Zuanning Yuan
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  3. Jingchuan Sun
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  4. Roxana Georgescu
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  5. Michael E. O’Donnell
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  6. Huilin Li
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Corresponding authors

Correspondence to Michael E. O’Donnell or Huilin Li .

Editor information

Editors and Affiliations

  1. Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

    Hisao Masai

  2. Istituto FIRC di Oncologia Molecolare, Milan, Italy

    Marco Foiani

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Bai, L., Yuan, Z., Sun, J., Georgescu, R., O’Donnell, M.E., Li, H. (2017). Architecture of the Saccharomyces cerevisiae Replisome. In: Masai, H., Foiani, M. (eds) DNA Replication. Advances in Experimental Medicine and Biology, vol 1042. Springer, Singapore. https://doi.org/10.1007/978-981-10-6955-0_10

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