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Soil Metagenomics: A Tool for Sustainable Agriculture

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Mining of Microbial Wealth and MetaGenomics

Abstract

Microorganisms are considered as an unlimited, untapped, and intriguing source for development of novel genes, antibiotics, bioinoculants, biocatalysts, etc. Therefore, the identification and characterization of the microbial wealth becomes inevitable. Besides other features of bacteria that are a challenge to health, including increase in pathogenicity, antibiotic resistance, mounting host spectra, and possibility of usage for bioterrorism, it becomes a compulsion. Furthermore, a sustainable agriculture development program that frequently exploits the plant growth-promoting rhizobacteria (PGPR) also requires a correct identification and characterization of agriculturally important microorganisms. Metagenomics is defined as the study of all available genomes in an environment that contains considerably more genetic information than provided by the cultured subset. It also helps in bridging the gap between genetics and ecology, indicating that the genes of a single microorganism are connected to the genes of other members of the community.

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References

  • Aakvik T, Degnes KF, Dahlsrud R, Schmidt F, Dam R, Yu L, Völker U, Ellingsen TE, Valla S (2009) A plasmid RK2–based broad–host–range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species. FEMS Microbiol Lett 296:149–158. doi:10.1111/j.1574-6968.2009.01639.x

    Article  CAS  PubMed  Google Scholar 

  • Albers SV, Jonuscheit M, Dinkelaker S, Urich T, Kletzin A, Tampe R, Driessen AJM, Schleper C (2006) Production of recombinant and tagged proteins in the hyperthermophilic archaeon Sulfolobus solfataricus. Appl Environ Microbiol 72:102–111. doi:10.1128/AEM.72.1.102-111.2006

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Angelov A, Mientus M, Liebl S, Liebl W (2009) A two–host fosmid system for functional screening of (meta) genomic libraries from extreme thermophiles. Syst Appl Microbiol 3:177–185. doi:10.1016/j.syapm.2008.01.003

    Article  Google Scholar 

  • Bailly J, Fraissinet-Tachet L, Verner MC, Debaud JC, Lemaire M, Wésolowski-Louvel M, Marmeisse R (2007) Soil eukaryotic functional diversity, a metatranscriptomic approach. ISME J 1:632–642. doi:10.1038/ismej.2007.68

    Article  CAS  PubMed  Google Scholar 

  • Bernstein JR, Bulter T, Shen CR, Liao JC (2007) Directed evolution of ribosomal protein S1 for enhanced translational efficiency of high GC Rhodopseudomonas palustris DNA in Escherichia coli. J Biol Chem 282:18929–18936. doi:10.1074/jbc.M701395200

    Article  CAS  PubMed  Google Scholar 

  • Craig JW, Chang FY, Kim JH, Obiajulu SC, Brady SF (2010) Expanding small–molecule functional metagenomics through parallel screening of broad–host–range cosmid environmental DNA libraries in diverse proteobacteria. App Environ Microbiol 76:1633–1641. doi:10.1128/AEM.02169-09

    Article  CAS  Google Scholar 

  • Delmont TO, Robe P, Clark I, Simonet P, Vogel TM (2011) Metagenomic comparison of direct and indirect soil DNA extraction approaches. J Microbiol Methods 86:397–400. doi:10.1016/j.mimet.2011.06.013

    Article  CAS  PubMed  Google Scholar 

  • Ferrari BC, Binnerup SJ, Gillings M (2005) Microcolony cultivation on a soil substrate membrane system selects for previously uncultured soil bacteria. Appl Environ Microbiol 71(12):8714–8720. doi:10.1128/AEM.71.12.8714-8720.2005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Handelsman J, Rondon MR, Brady SF, Clardy J, Goodman RM (1998) Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol 5:245–249. doi:10.1016/S1074-5521(98)90108-9

    Article  Google Scholar 

  • Illeghems K, Weckx S, Vuyst LD (2015) Applying meta-pathway analyses through metagenomics to identify the functional properties of the major bacterial communities of a single spontaneous cocoa bean fermentation process sample. Int J Food Microbiol 50:54–63. doi:10.1016/j.fm.2015.03.005

    Article  CAS  Google Scholar 

  • Jones JG (1977) The effect of environmental factors on estimated viable and total populations of planktonic bacteria in lakes and experimental enclosures. Freshwater Biol 7:67–91. doi:10.1111/j.1365-2427.1977.tb01659.x

    Article  CAS  Google Scholar 

  • Lämmle K, Zipper H, Breuer M, Hauer B, Buta C, Brunner H, Rupp S (2007) Identification of novel enzymes with different hydrolytic activities by metagenome expression cloning. J Biotechnol 127:575–592. doi:10.1016/j.jbiotec.2006.07.036

    Article  PubMed  Google Scholar 

  • Liu Y, Yao T, Jiao N, Kang S, Xu B, Zeng Y, Huang S, Liu X (2009) Bacterial diversity in the snow over Tibetan Plateau Glaciers. Extremophiles 13:411–423. doi:10.1007/s00792-009-0227-5

    Article  CAS  PubMed  Google Scholar 

  • McGarvey KM, Queitsch K, Fields S (2012) Wide variation in antibiotic resistance proteins identified by functional metagenomic screening of a soil DNA library. Appl Environ Microbiol 78:1708–1714. doi:10.1128/AEM.06759-11

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nacke H, Will C, Herzog S, Nowka B, Engelhaupt M, Daniel R (2011) Identification of novel lipolytic genes and gene families by screening of Metagenomic libraries derived from soil samples of the German Biodiversity Exploratories. FEMS Microbiol Ecol 78:188–201. doi:10.1111/j.1574-6941.2011.01088.x

    Article  CAS  PubMed  Google Scholar 

  • Pham VH, Kim J (2012) Cultivation of unculturable soil bacteria. Trends Biotechnol 30(9):475–484. doi:10.1016/j.tibtech.2012.05.007

    Article  CAS  PubMed  Google Scholar 

  • Pradhan S, Srinivas TNR, Pindi PK, Kishore KH, Begum Z, Singh PK, Singh AK, Pratibha MS, Yasala AK, Reddy GS, Shivaji S (2010) Bacterial biodiversity from Roopkund glacier, Himalayan mountain ranges, India. Extremophiles 14:377–395. doi:10.1007/s00792-010-0318-3

    Article  CAS  PubMed  Google Scholar 

  • PremaLatha K, Soni R, Khan M, Marla SS, Goel R (2009) Exploration of csp gene(s) from temperate and glacier soils of Indian Himalaya and in silico analysis of encoding proteins. Curr Microbiol 58:343–348. doi:10.1007/s00284-008-9344-0

    Article  Google Scholar 

  • Raynaud X, Nunan N (2014) Spatial ecology of bacteria at the microscale in soil. PLoS One 9(1):e87217. doi:10.1371/journal.pone.0087217

    Article  PubMed  PubMed Central  Google Scholar 

  • Shivaji S, Pratibha MS, Sailaja B, Kishore KH, Singh AK, Begum Z, Anarasi U, Prabagaran SR, Reddy GSN, Srinivas TNR (2011) Bacterial diversity of soil in the vicinity of Pindari glacier, Himalayan mountain ranges, India, using culturable bacteria and soil 16S rRNA gene clones. Extremophiles 15:1–22. doi:10.1007/s00792-010-0333-4

    Article  CAS  PubMed  Google Scholar 

  • Soni R, Goel R (2010) Triphasic approach for assessment of bacterial population in different soil systems. Ekologija 56:94–98. doi:10.2478/v10055-010-0014-8

    Article  CAS  Google Scholar 

  • Soni R, Goel R (2011) nifH homologous from soil metagenome. Ekologija 57:87–95. doi:10.6001/ekologija.v57i3.1914

    Article  CAS  Google Scholar 

  • Sørensen HP, Mortensen KK (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115:113–128. doi:10.1016/S0167-7799(97)01155-4

    Article  PubMed  Google Scholar 

  • Surmann E-M, Efferth T (2014) Biodiversity and metagenomics. In: Kuete V, Efferth T (eds) Biodiversity natural, products and cancer treatment. World Scientific Publishing Co., Singapore, pp 35–69. doi:10.1142/9789814583510_0002

    Google Scholar 

  • Suyal DC, Yadav A, Shouche Y, Goel R (2015a) Bacterial diversity and community structure of Western Indian Himalayan red kidney bean (Phaseolus vulgaris L.) rhizosphere as revealed by 16S rRNA gene sequences. Biologia 70:305–313. doi:10.1515/biolog-2015-0048

    Article  CAS  Google Scholar 

  • Suyal DC, Yadav A, Shouche Y, Goel R (2015b) Diversified diazotrophs associated with the rhizosphere of Western Indian Himalayan native red kidney beans (Phaseolus vulgaris L.) 3Biotech 5:433–441. doi:10.1007/s13205-014-0238-5

    Google Scholar 

  • Treusch AH, Leininger S, Kletzin A, Schuster SC, Klenk HP, Schleper C (2005) Novel genes for nitrite reductase and Amo–related proteins indicate a role of uncultivated mesophilic crenarchaeota in nitrogen cycling. Environ Microbiol 7:1985–1995. doi:10.1111/j.1462-2920.2005.00906.x

    Article  CAS  PubMed  Google Scholar 

  • Troeschel SC, Drepper T, Leggewie C, Streit WR, Jaeger KE (2010) Novel tools for the functional expression of metagenomic DNA. Methods Mol Biol 668:117–139. doi:10.1007/978-1-60761-823-2_8

    Article  CAS  PubMed  Google Scholar 

  • Wang GY, Graziani E, Waters B, Pan W, Li X, McDermott J, Meurer G, Saxena G, Andersen RJ, Davies J (2000) Novel natural products from soil DNA libraries in a streptomycete host. Org Lett 10:2401–2404. doi:10.1021/ol005860z

    Article  Google Scholar 

  • Zhang X, Yao T, Tian L, Xu S, An L (2008) Phylogenetic and physiological diversity of bacteria isolated from Puruogangri ice core. Microbiol Ecol 55:476–488. doi:10.1007/s00248-007-9293-3

    Article  CAS  Google Scholar 

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Acknowledgment

The work mentioned in this chapter from author group was supported by the National Bureau of Agriculturally Important Microorganisms, India (NBAIM/ICAR), grant to R.G.

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Correspondence to Ravindra Soni .

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Goel, R., Suyal, D.C., Narayan, Dash, B., Soni, R. (2017). Soil Metagenomics: A Tool for Sustainable Agriculture. In: Kalia, V., Shouche, Y., Purohit, H., Rahi, P. (eds) Mining of Microbial Wealth and MetaGenomics. Springer, Singapore. https://doi.org/10.1007/978-981-10-5708-3_13

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