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Mussel (Mytilus coruscus) Water Extract Containing Taurine Prevents LPS-Induced Inflammatory Responses in Zebrafish Model

  • Conference paper
Taurine 10

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 975))

Abstract

Mussel (Mytilus coruscus) water extract had strong anti-inflammatory activities, but the effects and its mechanisms of mussel on anti-inflammatory properties in vivo remain to be determined. This study, therefore, was designed to investigate anti-inflammatory activities of mussel water extract containing a large amounts of taurine (151.96 nmol/mg) using the lipopolysaccharide (LPS)-induced inflammatory zebrafish model. In this study, mussel water extract containing taurine shows potent protective effects against the cell death stimulated by LPS exposure in zebrafish embryos. In addition, zebrafish subjected to LPS treatment exhibited significantly increased reactive oxygen species (ROS) and nitric oxide (NO) levels. However, mussel water extract markedly suppressed LPS-induced ROS and NO production. Our results indicate that mussel water extract attenuated inflammation by inhibiting the LPS-induced intracellular ROS and NO production in zebrafish embryos. These findings could demonstrate the anti-inflammatory activity of mussel water extract containing taurine, which might have a protective effects on inflammatory diseases.

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Abbreviations

AIA:

Adjuvant-induced arthritis

CIA:

Collagen-induced arthritis

Cys:

Cysteine

DAF-FM-DA:

Diaminofluorophore 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate

DCF:

Dichlorofluorescein

DCF-DA:

2′,7′-Dichlorodihydrofluorescein diacetate

LPS:

Lipopolysaccharide

Met:

Methionine

NO:

Nitric oxide

NOS:

Nitric oxide synthase

iNOS:

Inducible NOS

ROS:

Reactive oxygen species

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Acknowledgement

This study was financially supported by Chonnam National University, 2016.

Author information

Authors and Affiliations

  1. Department of Marine Bio Food Science, College of Fisheries and Ocean Science, Chonnam National University, Yeosu, 550-749, South Korea

    Sun Hee Cheong

  2. Division of Food Bioscience and Korea Nokyong Research Center, Konkuk University, Chungju, 27478, South Korea

    Seung-Hong Lee

  3. Department of Marine Life Science, Jeju National University, Jeju, 63243, South Korea

    You-Jin Jeon

  4. College of Pharmacy, Chosun University, Dong-gu, Gwangju, 61452, South Korea

    Dong-Sung Lee

Authors
  1. Sun Hee Cheong
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  2. Seung-Hong Lee
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  3. You-Jin Jeon
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  4. Dong-Sung Lee
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Corresponding author

Correspondence to Dong-Sung Lee .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, University of Seoul, Seoul, South Korea

    Dong-Hee Lee  & Ha Won Kim  & 

  2. College of Medicine, University of South Alabama, Mobile, Alabama, USA

    Stephen W. Schaffer

  3. Department of Developmental Neurobiology, NY State Institute for Basic Research in Developmental Disabilities, New York, USA

    Eunkyue Park

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Cheong, S.H., Lee, SH., Jeon, YJ., Lee, DS. (2017). Mussel (Mytilus coruscus) Water Extract Containing Taurine Prevents LPS-Induced Inflammatory Responses in Zebrafish Model. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_74

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