Skip to main content

Somatic Embryogenesis in Fruit and Forest Trees of Arid Zone

  • Chapter
Somatic Embryogenesis in Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 67))

Abstract

The application of tissue culture methods to the propagation of plants has dramatically altered the way plants are routinely propagated. As tissue culture technology continues to expand and improve, these techniques will be applied to the growing list of plant species. Most of the species that are presently being propagated on a large-scale through tissue culture are herbaceous, ornamental, fruit and forest trees. This technology provides the opportunity to produce in large number uniform, disease-free arid zone hardy plants where lack of method for vegetative propagation, uniformity and/ or virus infestation had been previously a serious problem. Tissue culture propagation is not likely to be applied in the near future to crops that are easily and satisfactorily propagated through other conventional methods. But there are a large number of species, many of which are important agronomically and horticultural crops, that are reproduce asexually and could get benefit from this technology. Unfortunately, conventional vegetative propagation practices can lead to the spread of plant pathogens, resulting in the loss of yield and quality. But, through tissue culture, methods for producing pathogen-free stock plants can successfully be generated and maintained in vitro, which ultimately increase the productivity on cultivation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Ajitkumar, D. and Seeni, S. 1998. Rapid clonal multiplication in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., A medicinal tree, Plant Cell Rept., 17: 422–426.

    Google Scholar 

  • Alexander, M. P. 1980. A versatile stain for pollen, fungi, yeast and bacteria, Stain Tech., 55: 13–18.

    CAS  Google Scholar 

  • Ammer, S. and Benbadis, A. 1977. Multiplication vegetative du palmier dattier (Phoenix dactylifera L.) par la culture de tissue de jeunes paints Issues de Semis, C. R. Acad. Sci. Series D., 284: 1789–1794.

    Google Scholar 

  • Ammimto, P. V. and Steward, F. C. 1971. Some effect of the environment on the development of embryos for cultured free cells. Bot. Gaz., 132: 149–158.

    Google Scholar 

  • Ammirato. P. V. 1983. Embryogenesis In: Handbook of Plant Cell Culture, Vol. I (D. A. Evans, W. R. Sharp, P. V. Ammerato, Yamada, eds.), Mc Milian Pub., New York, pp. 32–123.

    Google Scholar 

  • Ammirato, P. V. 1974. The effect of abscisic acid on the development of somatic embryos from cells of caraway (Carton carvi L.). Bot. Gaz., 135: 328–337.

    Google Scholar 

  • Arumugam, S. and Rao, M.V. 1996. In vitro production of plantlets from cotyledonary node cultures of Aegle marmelos ( L.) Corr. Advances in Plant Sciences, 9: 181–186.

    Google Scholar 

  • Arya, H. C., Ramawat, K. G. and Suthar, K. C. 1981. Culture and differentiation of plants of economic importance II. Aegle marntelos, J. Ind. Bot. Soc., 60: 134–137.

    Google Scholar 

  • Bapat, V. A., Fulzele, D. P-, Heble, M. R. and Rao, P. S. 1990. Production of sandalwood somatic embryos in bioreacter, Curt. Sci., 59: 746–748.

    Google Scholar 

  • Bayliss, M. W. 1980. Chromosomal variations in cultures. In: Perspective in Plant Cell and Tissue Culture, (I. K. Vasil, ed.), Int. Rev. Cytol. Sppl., 11A: 113–114.

    Google Scholar 

  • Becwar, M. R., Noland, T. L. and Wann, S. R. 1987. A method for quantification of the level of somatic embryogenesis among Norway spruce callus line, Plant Cell Rept., 6: 35–38.

    Google Scholar 

  • Ben-Hayyim, G. and Neumann, H. 1983. Stimulatory effect of glycerol on growth and somatic embryogenesis in citrus callus cultures, Z. Pflanzenphysiol, 110: 331–338.

    CAS  Google Scholar 

  • Bhandari, M.M. 1990. Flora of Indian desert, M P S Repros., Jodhpur, India, pp. 1–435.

    Google Scholar 

  • Bhaskaran, S. and Smith, R. H. 1992. Somatic embryogenesis from shoot-tip and immature inflorescence of Phoenix dactylifera cv. Barhee, Plant Cell Rept., 12: 22–25.

    Google Scholar 

  • Bitters, W. P., Murashige, T., Rangan, T. S. and Nauer, E. 1970. Investigations on established virus-free through tissue culture. California Citrus Nursery Soc., 9; 27–30.

    Google Scholar 

  • Bryant, J. A. and Cuming, A. C. 1993. Molecular control of development, In: Biochemistry and molecular biology (P. J. Lee and R. C. Leegood, eds. ), John Wiley and Sons Ltd., pp. 241–273.

    Google Scholar 

  • Chandra, A., Chandra, A. and Gupta, I.C. 1994. Arid Fruit Research, Scientific Publishers, Jodhpur.

    Google Scholar 

  • Chsan, R. 1993. Embryogenesis: New molecular insights. The Plant Cell, 8: 597–599.

    Google Scholar 

  • Chaturvedi, H. C. and Sharma, A. K. 1985. Production of androgenic plants of Citrus aurantifolia, J. Pl. Physiol., 119: 473–477. Chaturvedi, H.C. and Mitra, G.C. 1974. Clonal propagation of citrus from somatic callus cultures, Hort Sci., 9: 118–120.

    Google Scholar 

  • Carmi, F., Pasquale, F. D. and Crescimanno, F. G. 1995. Somatic embryogenesis in citrus from syles cultures, Plant Sciences, 105: 81–86.

    Google Scholar 

  • Das, A. B., Rout, G. R. and Das, P. 1995. In vitro somatic embryogenesis from callus cultures of timber yielding tree Hardwickia binata Roxb., Plant Cell Rept.. 15: 147–149.

    Google Scholar 

  • Das, P., Samantaray, S., Roberts, A.V. and Rout, G. R. 1997. In vitro somatic embryogenesis of Dalbergia sissoo Roxb: A multipurpose timber yielding tree, Plant Cell Rept., 16: 578–582.

    Google Scholar 

  • Dass, H. C., Kaul. R. K., Joshi, S. P. and Raj Bhansali, R. 1989. In vitro regeneration of date palm plantlets, Curt. Sci., 58: 22–24.

    Google Scholar 

  • Driver, J. A. and Kuniyuki, A. H. 1984. In vitro propagation of paradox walnut rootstock, Hort Sci., 19:507–509.

    Google Scholar 

  • Dodeman, V. L., Ducreux, G. and Kreis, M. 1997. Zygotic embryogenesis versus somatic embryogenesis, J. Exp. Bot., 48: 1493–1509.

    Google Scholar 

  • Drira, N. 1983. Multiplication vegetative du palmier (Phoenix dactylifera L.) par la culture in vitro de bourgons axillaires et de feuilles qui en dervant, C. R. Acad. Sci. Paris, 296: 1077–1082.

    Google Scholar 

  • Durieu, H. and Barber, M. 1982. High frequency of genetic variant plants regenerated from cotyledons of tobacco. In: Variability in Plants Regenerated from Tissue Culture, ( E. D. Earle and Y. Demarley, eds.), Praeger, New York. pp. 221–227.

    Google Scholar 

  • Eeswara, J.P., Stuchbury, T., Allan, E.J. and Mordue, A. J. 1998. A standard procedure for micropropagation of neem tree (Azadirachta indica A. Jass. ), Plant Cell Rept., 17: 215–219.

    Google Scholar 

  • Evans, D. A. Sharp, W. R. and Flick, C. E. 1981. Growth and behaviour of cell cultures: Embryogenesis and organogenesis. In: Plant Tissue Culture Methods and Application in Agriculture, (T. A. Thorpe, cd.), Academic Press, New York, PP. 45–113.

    Google Scholar 

  • Faroda, A.S., Singh, H.P., Singh, M., Lodha, S. Kumar, S., Bhati, T.K. and Harsh, L.N. 1997. Vision-2020-Central Arid Zone Research Institute Perspective Plan, Indian Council Agricultural Research, New Delhi, 1–62.

    Google Scholar 

  • Finer, J. J. 1994. Plant regeneration via embryogenic suspension cultures, In: Plant tissue culture: a pratical approach, (R. A. Dixon. and R. A. Gonzales, eds.), Oxford Press, Oxford, pp. 67–102.

    Google Scholar 

  • Fridborg, G., Pederson, M, Landstom, L.E. and Eriksson, T. 1978. The effect of activated charcoal on tissue cultures: Absorption of metabolites inhibiting morphogenesis. Physiol. Plant, 43: 104–106.

    Google Scholar 

  • Gamborg, O. L., Miller, R. A. and Ojima, K. 1968. Nutrient requirement of suspension cultures of soybean root cells, Exp. Cell Res. 50: 151–158.

    Google Scholar 

  • Garg, L., Bhandari, N. N., Rani, V. and Bhojwani, S. S. 1996. Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica, Plant Cell Rept., 15: 855–858.

    CAS  Google Scholar 

  • Gill, R.I.S., Gill, S.S. and Goyal. S. S. 1994. Protocol for in vitro asexual multiplication of Azadirachta indica. In: Recent Advances in Biotechnological Application of Plant Tissue and Cell, ( G. A. Ravishanker and V. Venkataraman, eds.), Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, pp. 156–160.

    Google Scholar 

  • Gharyal, P. K. and Maheshwari, S. C. 1981. In vitro differentiation of somatic embryos in leguminous tree, Albizia lebbek L. Naturwissenschaften, 68: 379–380.

    Google Scholar 

  • Goodin, J.R. and Northington, D.K. 1985. Plant resource of arid and semi-arid lands - A global perspective, Academic Press Inc., New York.

    Google Scholar 

  • Gupta, P. K. and Durian, D. J. 1985. Biotechnology of somatic embryogenesis and plantlet regeneration in loblolly pine. Bio/Tech. 5: 147–151.

    Google Scholar 

  • Gupta, P.K., Timmis, R., Pullman, G., Yancy, M., Kreitinger, M., Carlson, W. and Carpenter, C. 1991. Develoipment of embryogenic system for automated propagation of forest trees. In: Scale-up and automation in plant propagation, Cell Culture and Somatic Cell Genetics of Plants, Vol. 8, ( I. K. Vasil, ed.), Academic Press Inc., New York, pp. 76–93.

    Google Scholar 

  • Goyal, Y. and Arya, H.C. 1985. Tissue culture of desert trees-U. Clonal multiplication of Zizyphus in vitro, J. Plant Physiol., 119: 399–404.

    CAS  Google Scholar 

  • Halperin, W. 1966. Alternative morphogenetic events in cell suspension, Am. J. Bot., 53: 443–453.

    Google Scholar 

  • Harms, C. T. and Potrykus, L 1980. Hormone inhibition of citrus protoplast released by coculturig with Nicotiana tobacum protoplasts-Its significance for somatic hybrids selection, Plant Sci. Lett., 19: 611–614.

    Google Scholar 

  • Hazanka, B. N., Nagaraju, V. and Parthasarthy, V. A. 1996. Organogenetic responses of bael (Aegle marmelos) micro shoots to benzylaminopurine, Ann. Plant Physiol., 10: 40–44.

    Google Scholar 

  • Heberle-Bors, E. 1980. Interaction of activated charcoal and iron chelates in anther cultures of Nicotiana and Atropa belladonna. Z. Pflanzenphysiol 99: 339–347.

    CAS  Google Scholar 

  • Homes, J. L. A. 1967. Influence de la concentration en glucose sur al development et al differentiation d’embryons formes dans tissus de canette cultives in vitro. In: Les Culturs de Tissus de Plantes, Coll. Nat. CNRS., Paris, pp. 49–60.

    Google Scholar 

  • Hossain, M., Karim, M. R., Isalam, R. and Jorder, O.I. 1993a. Plant regeneration from nucellar tissues of Aegle marinelos through organogenesis, Plant Cell, Tiss. Org. Cul„ 34: 199–203.

    Google Scholar 

  • Hussain, M., Karim, M.R., Islam, R. and boarder, O. I. 1993b. Aventitious shoot formation on nucellus, embryonic tissues and seedlings of Aegle marmelos Con., Intern. Plant Tissue Culture Conf., Deptt of Bot., Dhaka Univ., Dhaka, December, 1921.

    Google Scholar 

  • Huber, J., Constabel, F. and Gamborg, O. L. 1978. A cell counting procedure applied to embryogenesis in cell suspensions of anise (Pimpinella anism L.), Plant Sci. Let., 12: 309–315.

    Google Scholar 

  • Islam, R., Hossain, M., Reza, M. A., Manton, A. N. K. and Jorder, O. L. 1996a Shoot regeneration from root-tips of intact seedlings of Aegel marmelos, J. Hort. Sci., 71: 995–1000.

    Google Scholar 

  • Islam, R., Hussain, M., Hogue, A. and boarder, O. L 1996b. Somatic embryogenesis and plant regeneration in Aegle marmelos-a multipurpose social tree, Curr. Sci. 71: 259–260.

    Google Scholar 

  • Islam, R., Karim, M. R., Rahman, S. M., Hussain, M. and Joarder, O. L 1994. Plant regeneration from excised cotyledons of Aegle marinelos Con., Pakistan J. Bot.. 26: 393–396

    Google Scholar 

  • Jaidka, K. and Mettra, P. N. 1986. Morphogenesis in Punica gran arum (pomegranate), Can. J. Bot., 64: 1644–1653.

    Google Scholar 

  • Jain, S. M., Newton, R. J. and Solta, B. J. 1988. Enhancement of somatic embryogenesis in Norway spruce (Picea abies). Theor. Appl. Genet., 76: 501–506.

    Google Scholar 

  • Jain, S. M., Dong, N. and Newton, R. J. 1989. Somatic embryogenesis in slash pine (Picea ellionii) from immature embryos. Plant Sci., 65: 233–241.

    Google Scholar 

  • Jain, S. M., Gupta, P. K. and Newton, R. J. 1995. Somatic embryogenesis in woody plants, Vol. 1–3, Kluwer Academic Publishers. The Netherlands.

    Google Scholar 

  • Jain, S. M., Gupta, P. K. and Newton, R. J. 1999. Somatic embryogenesis in woody plants. Vol. 4–5, Kluwer Academic Publishers, The Netherlands.

    Google Scholar 

  • Jumin, B. H. and Nito, N. 1996. Plant regeneration via somatic embryogenesis from protoplasts of six plant species related to Citrus, Plant Cell Reptr., 15: 332–336.

    CAS  Google Scholar 

  • Jufgens, G. and Mayer, U. 1992. Arabidopsis. In: Embryos: A colour atlas of developing embryos, (J. Bard, ed.), London, Wolfe. Kabir, A., Islam, R., Hogue, A., Khalekuzzansan, M. and boarder, O.A. 1994. Micropropagation of Zizyphus mauritiana from nodal segments of mature trees, Butl.Seri. Cult. Res., 5.

    Google Scholar 

  • Kochba, J. and Button, J. 1974. The stimulation of somatic embryogenesis and embryoid development in habituated ovular callus from the ‘Shamouti’ orange (Citrus sinensis) as affected by tissue age and sucrose concentration, Z. Pflanzenphysiol, 73: 415–421.

    Google Scholar 

  • Kochba, J. Spiegel-Roy, P., Neuman, H. and Saad. 1978. Stimulation of somatic embryogenesis in citrus ovular callus by ABA, ethiophon, CCC and ALAR and its suppression by GA, Z. Pflanzenphysiol, 89: 427–432.

    Google Scholar 

  • Kohlenbach, H.W. 1978. Comparative somatic embryogenesis In: Frontiers of Plant Tissue Culture, University of Calgary, pp. 55–66.

    Google Scholar 

  • Kononowicz, A. K. and Janik, J. 1984. The influence of carbon on the growth and development of asexual embryos of Theobroma cacao, Physiol. Plant., 61: 155–162.

    Google Scholar 

  • Krishnamurthy, K. V. 1999. On embryos and embryoids, Curr. Sci., 76: 647–659.

    Google Scholar 

  • Kurtz, S. L, Hartman, R. D. and Chu, Y. E. L 1991. Current methods of commercial micropropagation, In: Cell Culture and Somatic Cell Genetics of Plants, Vol. 8. Academic Press. New York, pp. 7–34.

    Google Scholar 

  • Larkin, P.J. and Scowcroft, J. M. 1981. Soma clonal variation-a novai source of variability from cell cultures for plant improvement. Theor. Appl. Genet., 60: 197–214.

    Google Scholar 

  • Lee, C. W., Thomas, J. C. and Rice, R. L. 1982. Jojoba oil production from embryo cultures. In: A. Elis-cesnick, ed. Proc. 5`“ Conf. Jojoba and its Uses, Tucson, pp. 9–20.

    Google Scholar 

  • Liu, M. C., Xu. Z. H. and Chua, N. H. 1993. Auxin polar transport is essential for establishment of bilateral symmetry during early plant embryogenesis, The Plant Cell, 5: 621–630.

    CAS  Google Scholar 

  • Litz, A. E. 1984. In vitro somatic embryogenesis from nucellar callus of monoembryogenic embryos, Hort. Sci., 19: 715–717.

    Google Scholar 

  • Lloyd, G. and Mc Cown, B. 1980. Commercially feasible micropropagation of mountain laurel (Kalmia larifolia) by use of shoot tip culture, Proc. Int. Plant Propagators Soc., 30: 421–427.

    Google Scholar 

  • Loh, C. S., Shu, W. and Khor, E. 1997. High frequency production of embryos from liquid flask cultures of oilseed rape, Biotechno. Bioeng., 54: 231–238.

    Google Scholar 

  • Lutz, J. D., Wong, J. R., Rowe, J., Tricoli, D. M. and Lawrence, R. H. 1985. Somatic embryogenesis for mass cloning of crop plants. Basic Life Sciences, 32: 105–116.

    Google Scholar 

  • Maheshwari, P. and Rangaswamy, N. S. 1958. Polyembryony andin vitro culture of citrus and Mangifera, Ind. Hort., 15: 275–286. Manohar, M.S. and Chandra, A. 1995. Date palm culture in Rajasthan, Directorate of Research, Rajalsthan Agricultural University, Bikaner.

    Google Scholar 

  • Mater, A. A. 1986. In vitro propagation of Phoenix dactvliJera L.. Date Palm J., 4: 137–151.

    Google Scholar 

  • Mathur, N., Ramawat, K. G. and Nandwani, D. 1995. Rapid /n vitro multiplication of jujube through mature seed explants, Plant Cell, Tiss. Org. Cultu., 43: 75–79.

    Google Scholar 

  • Medha, S., Thengane, S. R. and Mascarenhas, A.F. 1993. Somatic embryogenesis and plant regeneration in Azadirachta indica A. Juss., In Vitro Cell Dev. Biol., 29: 38–42.

    Google Scholar 

  • Meinke, D. W. 1995. Molecular genetics of plant embryogenesis. Ann. Rev. Plant Physiol. and Mole. Biol., 46: 369–394, Meins, F. 1983. Heritable variations in plant cell cultures. Ann.. Rev. Physiol., 37: 327–346.

    Google Scholar 

  • Mitra, G. C. and Chaturvedi, H. C. 1972. Embryoids and complete plants from unpollinated overy and from ovules of in vivo-grown emasculated flower buds of citrus, Supp. Tony Bot. Club, 99: 184–189.

    Google Scholar 

  • Mitrofanova, I.V., Mitrofanova, O. V. and Ponderi, D. K. 1997 Somatic embryogenesis and plant regeneration in Zizyphuc jujuba Mill. in vitro, Russian J. Plant Physiol., 44: 84–94.

    Google Scholar 

  • Moore. G. A. 1985. Factors affecting in vitro embryogenesis from under developed ovules of mature Citrus fruit. J. Am. Soc. Hort. Sci., 110: 66–70.

    Google Scholar 

  • Monier, M. 1990. Induction of embryogenesis in suspension culture, In: Methods in Molecular Biology Vol. 6: Plant Cell and Tissue Culture, ( J. M. Pollard and J. M. Walker, eds.), Humana Press, New Jersey. pp. 149–157.

    Google Scholar 

  • Mouras, A. and Lutz, A. 1980. Induction, repression et conversion des proprietes embryogenetiques des cultures des tissus de carotte sauvage, Bull. Soc. Bot., ( French ) 127: 93–97.

    Google Scholar 

  • Muralidharan, E. M. and Mascarenhas, A. F. 1987. In vitro planlet formation by organogenesis in E. canwdulensis and by somatic embryogenesis in Eucalyptus citriodora,Plant Cell Rept., 6: 256–259.

    Google Scholar 

  • Muralidharan, E. M., Gupta. P. K. and Mascarenhas, A. F. 1989. Plantlet production through high frequency somatic embryogenesis in long-term cultures of Eucalyptus citridara, Plant Cell Rept., 8: 41–43.

    Google Scholar 

  • Murashige, T. 1974. Plant propagation through tissue cultures, Ann. Rev. Plant Physiol., 25: 135–166.

    Google Scholar 

  • Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 15: 473–497.

    Google Scholar 

  • Murthy, N.S. and Saxena, P.K. 1998. Somatic embryogenesis and plant regeneration in neem (Azadiraachra indica A. Juss. ), Plant Cell Rept., 17: 469–473.

    Google Scholar 

  • Nandwani, D. and Ramawat, K. G. 1993. In vitro plantlet formation through juvenile and mature explants in Prosospis cineruria. Ind. Exp. Biol. 31: 156–160.

    Google Scholar 

  • Nandwani, D. 1995. Micropropagation of Acacia tartilis adapted to arid soils, Ann. Sci. For., 52. 183–189.

    Google Scholar 

  • Nataraja, K. and Neelambica, G.K. 1996. Somatic embryhogenesis and plantlet from petal cultures of pomegranate, Punica granatum L., Indian J. Exp. Biol., 34 719–721.

    Google Scholar 

  • Navarro, L. 1981. Citrus shoot-tip grafting in vitro (STG) and its application: A Review, Proc. Intern. Soc. Citriculture pp. 452–456.

    Google Scholar 

  • Navarro, L. and Juarez, J. 1977. Tissue culture techniques used in Spain to recover virus free culture plants, Acta Hort., 78: 425–435.

    Google Scholar 

  • Nito, N. and Iwamasa, M. 1990. In vitro plantlet formation from juice vesicle callus of satsuma (Citrus unshiu, Mare ), Plant Cell Tiss. Org. Cul., 20 137–140.

    Google Scholar 

  • Oghawara, T., Kobayashi, S., Ishii, H., Yoshinaga, K. and Oiyama, I. 1989 Somatic hybridization in Citas novai orange (C. sinensi.s Osb.) and grapefruit (C. paradisi Macf.), Theor. Appt Genet., 78: 609–612.

    Google Scholar 

  • Ohta, Y. and Furusato, K. 1967. Embryo culture in citrus, Seiken Ziho, 8: 49–54.

    Google Scholar 

  • Omura, M., Matsuta, N., Moriguchi, T., Kozaki, I. and Sanada, T. 1987a. Establishment of tissue culture methods of dwarf pomegranate (Punica granatum L var. Nana and application for induction of varieties, Bull. Fruit Tree Res. Stn. Japan A. 14: 17–44.

    Google Scholar 

  • Omura, M., Matsuta, N.. Morikguchi, T. and Kozaki, I. 1987b. Adventitious shoots and plantlet formation from cultured pomegranate leaf explants. Hort. Sci., 22: 133–134.

    Google Scholar 

  • Oppenheimer, C. and Reuveni, O. 1972. Development of a method for quick propagation of new and superior date varieties, The Valconi Centre, Bet-Dagan, Israel, pp. 1–92.

    Google Scholar 

  • Osuga, K. and Komamaine. A. 1994. Synchronization of somatic embryogenesis from carrot cells at high frequecy as a basis for mass production of embryos, Plant Cell Tiss. Org. Cul., 39: 125–135.

    Google Scholar 

  • Pareek, O. P. and Nath, V. 1996. Coordinated fruit research in Indian arid zone, National Research Centre for Arid Fruits, Bikaner. pp 1–104.

    Google Scholar 

  • Pareek, O.P. 1997. Arid horticulture: its potential, Indian Horticulture, 42: 42–46.

    Google Scholar 

  • Pareek, O. P. 1996. Indian jujube and pomegranate, In: 50 Years of Crop Science Research in India ( R. S. Paroda and K. L. Chadha, eds.), Indian Council Agricultural Reseach, New Delhi, pp. 557–573.

    Google Scholar 

  • Parada, R.S. 1979. Plant resources of Indian arid zones for industrial uses. In: Arid Land Plant Resources, pp. 261–281 ( J.R. Goodin and D.K. Northington, eds.), ICA and SAIS, Texas Technology University, Lubbock, Texas.

    Google Scholar 

  • Parathasarthy, V. A. 1993. Embryogenesis in ovules of Citrus reticulate ( Blanco ), Ind J. Hort., 50: 103–108.

    Google Scholar 

  • Parrhasarhy, V. A. and Nagaraju, V. 1992. Effect of growth regulators and organic supplementation on embryogenesis in two Citrus species, J. Tree Sci., 11: 146–148.

    Google Scholar 

  • Parathasarthy, V. A. and Nagaraju, V. 1994. Effect of NAA and malt extract on in vitro growth of excised embryos of Citrus, Ind. J. Trop. Agric., 12: 107–111.

    Google Scholar 

  • Pierik, R. L. M. 1971. Plant tissue culture as motivation for the symposium, In: Effect of sterilization on components in nutrient media, (J. V. Bragt, D. A. A. Mossel., R.L.M. Pierik and H. Veldstra, eds.), Veenman, H. and Zonen, N. V., Wageningen, The Netherlands, pp. 3–14.

    Google Scholar 

  • Rai, V. R. 1993. Embryogenesis through cell suspension culture of forest tree: Dalbergia lattjolia Roxb., Plant Cell Rept., 8: 23–27.

    Google Scholar 

  • Raj Bhansali, R. 1989. Micropropagation of temperate and tropical fruit trees through repetitive somatic embryogenesis, XII Plant Tissue Culture Conf., North-Eastern Hill University, Shillong, p. 16.

    Google Scholar 

  • Raj Bhansali, R. 1990. Somatic embryogenesis and regeneration of plantlets in pomegranate, Ann. Bot., 66: 249–1253.

    Google Scholar 

  • Raj Bhansali, R. 1993a. Bud culture of shoot multiplication and plantlet formation of Tecomella undulata ( Rohida) woody tree of arid zone, Tropical Science 33: 1–8.

    Google Scholar 

  • Raj Bhansali, R. 1993b. In vitra selection of disease resistant embroygenic cell lines of date palm by using pathotoxins. International Conference on Biotechnology in Agriculture and Forestry. Kanpur, India.

    Google Scholar 

  • Raj Bhansali, R. 1994. Micropropagation of temperate and tropical fruit trees through somatic embryogenesis. In: Advances in Plant Tissue Culture in India, ( P. Tandon, ed.), Pragati Prakashan, Meerut, pp. 136–144.

    Google Scholar 

  • Raj Bhansali, R. 1995. A Manual of Plant-Tissue Culture (Prepared for the short-term training course), Department of Biotechnology, New Delhi, pp. 1–122.

    Google Scholar 

  • Raj Bhansali, R. 1998. Repetitive somatic embryogenesis and enhanced axillary proliferation methods for multiplication of arid zone fruit plants. Intern. Seminar on Micropropagation and Spread of Superior Genetic Material of Forest Trees, WWF., New Delhi, p. 17.

    Google Scholar 

  • Raj Bhansali, R. 1999. Biotechnology in arid agriculture and forestry. In: Research and Development Priorities for Md Zone

    Google Scholar 

  • Beyond 2000 A.D., (S. K. Vanna, R. Raj Bhansali, S. K. Jindal, S. Vir eds.), Scientific Publishers, Jodhpur, (in press).

    Google Scholar 

  • Raj Bhansali, R. and Arya, H.C. 1978a. Differentiation in Citrus paradisi Macf (Grapefruit) grown in culture, Indian J. Expt Biol. 16: 409–410.

    Google Scholar 

  • Raj Bhansali, R. and Arya, H.C. 1978b. Shoot formation in stem and root callus of Citrus aurantifolia ( Christuri) Swingle grown in culture, Curr. Sci. 47: 780–781.

    Google Scholar 

  • Raj Bhansali, R. and Arya, H.C. 1978c. Tissue culture propagation of Citrus trees. Proc. Intern. Soc. Citri., p. 135–140.

    Google Scholar 

  • Raj Bhansali, R. and Arya, H.C. 1979. Organogenesis in Citrus limettoides Tanaka ( Sweet lime) callus culture, Phytomorphology, 28: 97–100.

    Google Scholar 

  • Raj Bhansali, R. and Singh, K. 1982. Callus and shoot formation from leaf of sugar cane in tissue cultures, Phytomorphology, 12: 167–170.

    Google Scholar 

  • Raj Bhansali, R. and Kaul, R.K. 1991. Into future - Date through tissue culture, Ind. Hort, 36: 6–10.

    Google Scholar 

  • Raj Bhansali, R., Driver, J. A. and Durzan D. J. 1990. Rapid multiplication of adventitious somatic embryos in peach and nectarine by secondary embryogenesis, Plant Cell Rept., 9: 280–284.

    Google Scholar 

  • Raj Bhansali, R., Driver, J. and Durian, D.J. 1991. Somatic embryogenesis in cell suspension cultures of Prunus persica (L.). J. Hort. Sci., 66: 601–605.

    Google Scholar 

  • Raj Bhansali, R., Jindal, S. K. and Satyavir 1995. Biotechnology of somatic embryogenesis and plant regeneration in Neem (Azadirachta indica A. Jess.)-A potential pesticidal tree of arid zone. National Symposium on Recent Advances in Plant Tissue Culture and Biotechnology, Jaipur, India. p. 31.

    Google Scholar 

  • Raj Bhansali, R., Kaul, R.K. and Dass, H.C. 1988. Mass cloning of date palm plantlets through repetitive somatic embryogenesis process, J. Plant Anat. and Morph., 5: 73–79.

    Google Scholar 

  • Rangan, T.S., Murashige, T. and Bitters, W.P. 1969. In vitro studies of zygotic and nucellar embryogenesis in citrus. Proc. 1e Intern. Citrus. Symp., University of California, Riverside, pp. 225–229.

    Google Scholar 

  • Rangan, T.S., Murashige, T. and Bitters, W.P. 1968. In vitro initiation of nucellar embryos in monoembryonic citrus, Hort Sci., 3: 226–227.

    Google Scholar 

  • Rao, M. M. and Laxmisita, G. 1996. Direct somatic Dalbergia lanfolia embryogenesis from immature embryos of resewood ( Roxb. ). Plant Cell Rept., 15: 355–359.

    Google Scholar 

  • Rao, P. S., Bapat, V. A. and Mhatre, M. 1984. Regulatory factors for in vitro multiplication of sandal wood tree (Santalum album Linn.), Proc. Ind. Nat. Acad. Sci. Bio., 50: 196–202.

    Google Scholar 

  • Rathore, T. S., Singh, R. P., Deora, N. S. and Sekhawat, N. S. 1992. Clonal propagation of Zizyphus species through tissue culture. Scientia Hort., 51: 165–168.

    CAS  Google Scholar 

  • Redenbaugh, K., Paasch, B., Nichol, J., Kossler, M., Viss, P. and Waker, K. 1986. Somatic seeds: Encapsulation of asexual plant embryos, Biotech., 4: 797–801.

    Google Scholar 

  • Redenbaugh, K., Fugii, A. and Slade, D. 1991. Sythetic seed technology. In: Scale-up and autamation in plant propagation, Cell Culture and Somatic Cell Genetics of Plants, Vol. 8 ( I. K. Vasil, ed.), Academic Press, New York, pp. 35–75.

    Google Scholar 

  • Reinert, J. 1958. Morphogenese und ihre kont rolle on Gewebekulturen aus carotten, Naturwiessenschaften 45: 344–345.

    CAS  Google Scholar 

  • Reinert, J. 1959. Uber die kontrolle der morphogenese unds die induktion von adveembryonem and gewebekulturen aus karotten. Planta, 58: 318–333.

    Google Scholar 

  • Reinert, J., Tazawa, M. and Semenoff, S. 1967. Nitrogen compound as a factor of embryogenesis in vitro, Nature, 216: 1215–1216.

    PubMed  CAS  Google Scholar 

  • Reynolnd, J. F. and Murashige, T. 1979. Asexual embryogenesis in callus cultutres of palm, In Vitro Cell Dev. Biol., 15: 383–387.

    Google Scholar 

  • Phillip, G. C., Hubstenberger, J. F. and Hennen, E. E. 1995. Plant regeneration from callus and cell suspension cultures by somatic embryogenesis, In: Plant cell, tissue and organ cultures-fundamental methods (O. L. Gamborg and G. C. Phillips, eds.) Narosa Pub. House, New Delhi, pp. 81–90.

    Google Scholar 

  • Rhiss, A., Poulin, C. and Beauchesne, G. 1979. La culture In vitro applique a la multiplication vegetative du palmier dattier (Phoenix dactylifera L), Fruits, 34: 551–554.

    Google Scholar 

  • Rout, G. R., Samantaray, S. and Das, P. 1995. Effect of growth regulators and cultural environment on somatic embryogenesis of Acacia catechu wild., Israel J. PI. Sci., 43: 263–269.

    Google Scholar 

  • Roy, S. K., Assaduzzaman, M. and Hossain, Z. 1994. Clonal propagation of Azadirachta indica by in vitro culture. In: Recent Advances in Biotechnological Application of Plant Tissue and Cell, ( G. A. Ravishanker and V. Venkataraman, eds.), Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, pp. 149–154.

    Google Scholar 

  • Schenk, R. U. and Hilderbrandt, A. C. 1972. Medium and technique for induction of growth of monocotyledonous and dicotyledonous plant tissue cultures, J. Exp. Bot., 50: 166–204.

    Google Scholar 

  • Singh, A. K., Suri, S. S. Ramawat, K. G. and Soni, K. C. 1997. Somatic embryogenesis from immature zygotic embryos of Commiphora wightii, a woody medicinal plant, Gartenbauwissenschaft, 62: 44–47.

    Google Scholar 

  • Sharma, A. K. and Sharma, A. 1972. Chromosome techniques, Butterworths, London.

    Google Scholar 

  • Shamra, D.R., Dawara, S. and Chowdhary, J.B. 1984. Somatic embryogenesis and plant regenemtion in date palm (Phoenix dactylifera Lin) cv. ‘Khadravi’ through tissue culture, Ind. J. Exp. Biol., 22: 596–598.

    Google Scholar 

  • Sharma, D.R., Deepak, S. and Chowdhary, J.B. 1986. Regeneration of plants from somatic tissues of date palm (Phoenix dactylifera Linn.), Ind. J. Exp. Biol. 24: 763–766.

    Google Scholar 

  • Sharp, W. R., Evans, D. A., Ammirato, P.V. and Yamada, Y. 1984. Handbook of plant cell culture, Vol. 2, Mac Milian, New York.

    Google Scholar 

  • Sharp, W. R., Sondahl. M. R., Clades, L. S. and Maraffa, S. B. 1980. The physiology of in vitro asexual embryogenesis. Hortic. Rev. 2: 268–310.

    Google Scholar 

  • Srivastava, P.S., lqbal. M. and Purohit, M. 1997. Biotechnological approaches in the improvement of Citrus, In: Role of Biotechnology in Aromatic and Medicinal Plants, Vol. I (L A. Khan, ed.), Ukaaz Publications, Hyderabad, pp. 199–209.

    Google Scholar 

  • Steward, F. C., Mapes, M. O. and Meare, K. 1958. Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells, Ans. J. Bot., 45: 705–708.

    Google Scholar 

  • Steward, F.C., Israel, H. W., Mott, R. L, Wilson, H. J. and Krikorian, A. D. 1975. Observations on growth and morphogenesis in cultured cells of carrot (Daucus carota L.), Philos. Trans. R. Soc. London Ser. B., 273: 33–53.

    Google Scholar 

  • Street, H. E. 1977. Plant tissue and cell culture, 2°d ed., University California Press, Berkeley.

    Google Scholar 

  • Sudhersan, C., Abo El Nil, M. M. and Al Bair, A. 1993. Occurrence of direct somatic embryogenesis on sword leaf in vitro plantlets of Phoenix dactylifera L cv. Barhee, Curr. Sci., 65: 687–689.

    Google Scholar 

  • Sung, D. O. and Song, W. L 1992. Regeneration of Chinese jujube (Zizyphus jujube Miller) from immature cotyledons and segements of seedling. Hort Sci., 27: 210.

    Google Scholar 

  • Tay, L F., Khoh, L K., Loh, C. S. and Khor, E. 1993. Alginate-chitosan conservation in production of artificial seeds. Biotechnol. Bioeng., 42: 449–454.

    Google Scholar 

  • Tisseras, B. 1984. Clonal propagation, In: Cell Culture and Somatic Cell Genetics of Plants, ( L K. Vasil, ed.), Academic Press, New York, pp. 74–80.

    Google Scholar 

  • Tisserat, B. 1987. Palms. In: Cell and Tissue Culture in Forestry ( J. M. Bonga and D. J. Durzan eds.), Martinus Nijhoff Publishers, Boston, USA, pp. 338–356.

    Google Scholar 

  • Tisseras, B. and Murashige, T. 1977. Repression of asexual embryogenesis in vitro by some plant growth regulators, In Vitro, 13: 799805.

    Google Scholar 

  • Tisseras, B., Foster, G. and De Mason, D. 1979. Plantlet production In vitro Phoenix dactylifera L., Dates Growers Rept., 54: 19–23.

    Google Scholar 

  • Toonen, M. A. J., Hendriks, T., Schmidt, E. D. I., Verhooven, H. A., van Kammen, A. and de Vries, S. C. 1994. Description of somatic embryo forming single cell in canut suspension cultures employing video cell tracking. Planta, 194: 565–572.

    CAS  Google Scholar 

  • Tulecke, W. 1987. Somatic embryogenesis in woody perennials. In: Cell and Tissue Culture in Forestry (J. M. Bongs and D. J. Durzan, eds ), Martinus Nijhoff Publication, pp. 61–91.

    Google Scholar 

  • Tusa, N., Grosser, J.W. and Gmitter, F. G. 1990. Plant regeneration of “Valencia” sweet orange. ‘Femminello’ lemon, and inter specific somatic hybrid following protoplast fusion, J. Am. Soc. Hort. Sci., 113: 1043–1046.

    Google Scholar 

  • Verma, D. C. and Dougall, D. K. 1977. Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures, Plant Physiol., 59: 81–85.

    PubMed  CAS  Google Scholar 

  • Vasil, I. K. 1985. Somatic embryogenesis and its consequences in the graminar. In: Tissue Culture in Forestry and Agriculture (R. Henke, K. Hughes, M. Constantin and A. Hollender, eds.), Plenum Press, Basic Life Sciences, 32: 31–47.

    Google Scholar 

  • Vasil, L K. 1991. Pint tissue culture and molecular biology as tool in understanding plant development and improvement, Current Opinios in Biotechnology, 2: 158–163.

    CAS  Google Scholar 

  • Vasil, V. and Vasil, L K. 1881. Somatic embryogenesis and plant regeneration from tissue cultures of pearl millet (Pennesetum americanum), Ann. Bot., 47: 669–678.

    Google Scholar 

  • Wang, H. and Chang, C. 1978. Triploid citrus plantlets from endosperm cultures. In: Proc. Syrup. Plant Tissue Culture, Science Press, Peking, pp. 463–467.

    Google Scholar 

  • Weatherhead, M. A., Burdon, J. A. and Henshaw, G. G. 1978. Some effect of activated charcoal as an adventive to plant tissue culture media, Z. Pflanzenphysiol., 89: 141–147..

    Google Scholar 

  • Wetherell, D. F. 1978. In vitro embryoid formation in cell derived from somatic plant tissues. In: Propagation of Higher Plants through Tissue Culture, Bridge between Research and Application (K. W. Hughes, R. Henke and M. Constaintin, eds.), Technical Information Centre, U. S. Department of Energy, Oak Ridge, pp. 102–124.

    Google Scholar 

  • Withers, L. and Alderson, P. 1986. Plant tiisue culture and its agricultural application, Butterworths, London.

    Google Scholar 

  • Zaid, A. 1987. In vitro browning of tissue and media with special reference to date palm cultures- A review, Acta Hort., 212: 56 1566.

    Google Scholar 

  • Zaid, A. and Tisseras, B. 1983. Morphogenetic responses obtained from a variety of somatic expiant tissue of date palm, Bot. Mag. ( Tokyo ), 96: 67–73.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Bhansali, R.R., Singh, M. (2000). Somatic Embryogenesis in Fruit and Forest Trees of Arid Zone. In: Jain, S.M., Gupta, P.K., Newton, R.J. (eds) Somatic Embryogenesis in Woody Plants. Forestry Sciences, vol 67. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3030-3_5

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-3030-3_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5508-8

  • Online ISBN: 978-94-017-3030-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics