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Experimental haploidy in Brassica species

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In Vitro Haploid Production in Higher Plants

Abstract

The genus Brassica comprises many familiar and commercially important food and oil-seed plants. Leaves, stems and roots have been cultivated as vegetables and animal fodder; both edible and industrial oils as well as condiments (mustards) have been prepared from the crushed seed.

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References

  • Arnison, P.G., P. Donaldson, L.C.C. Ho and W.A. Keller, 1990a. The influence of various physical parameters on anther culture of broccoli (Brassica oleracea var. italica). Plant Cell Tiss. Org. Cult. 20: 147–155.

    Google Scholar 

  • Arnison, P.G., P. Donaldson, A. Jackson, C. Semple and W.A. Keller, 1990b. Genotype-specific response of cultured broccoli (Brassica oleracea var. italica) anthers to cytokinins. Plant Cell Tiss. Org. Cult. 20: 217–222.

    CAS  Google Scholar 

  • Arnison, P.G. and W.A. Keller, 1990. A survey of the anther culture response of Brassica oleracea L. cultivars grown under field conditions. Plant Breed. 104: 125–133.

    Google Scholar 

  • Aslam, F.N., M.V. MacDonald, P.T. Loudon and D.S. Ingram, 1990a. Rapid-cycling Brassica species: Inbreeding and selection of Brassica napus for anther culture ability and an assessment of its potential for microspore culture. Ann. Bot. 66: 331–339.

    Google Scholar 

  • Aslam, F.N., M.V. MacDonald and D.S. Ingram, 1990b. Rapid-cycling Brassica species: Anther culture potential of B. campestris L. and B. napus L. New Phytol. 115: 1–9.

    Google Scholar 

  • Aspinall, D. and L.G. Paleg, 1981. Proline accumulation: Physiological aspects. In: L.G. Paleg and D. Aspinall (Eds.), Physiology and Biochemistry of Drought Resistance in Plants, pp. 205–241. Academic Press, Sydney.

    Google Scholar 

  • Bagga, S., N. Bhalla-Sarin, S.K. Sopory and S. Guha-Mukherjee, 1982. Comparison of in vitro plant formation from somatic tissues and pollen grains in Brassica oleracea var. botrytis. Phytomorphology 32: 152–156.

    Google Scholar 

  • Baillie, A.M.K., D.J. Epp, D. Hutcheson and W.A. Keller, 1992. In vitro culture of isolated microspores and regeneration of plants in Brassica campestris. Plant Cell Rep. 11: 234–237.

    Google Scholar 

  • Banga, S.S and K.S. Labana, 1986. Spontaneous haploidy in Brassica napus. Cruciferae Newsl. No. 11: 54–55.

    Google Scholar 

  • Biddington, N.L., 1992. The influence of ethylene in plant tissue culture. Plant Growth Regul. 11: 173–187.

    CAS  Google Scholar 

  • Biddington, N.L. and H.T. Robinson, 1990. Variations in response to high temperature treatments in anther culture of Brussels sprouts. Plant Cell Tiss. Org. Cult. 22: 48–54.

    Google Scholar 

  • Biddington, N.L. and H.T. Robinson, 1991. Ethylene production during anther culture of Brussels sprout (Brassica oleracea var. gemmifera) and its relationship with factors that affect embryo production. Plant Cell Tiss. Org. Cult. 25: 169–177.

    Google Scholar 

  • Biddington, N.L., H.T. Robinson and J.R Lynn, 1993. ABA promotion of ethylene production in anther culture of Brussels sprouts (Brassica oleracea var. gemmifera) and its relevance to embryogenesis. Physiol. Plant 88: 577–582.

    Google Scholar 

  • Biddington, N.L., R.A. Sutherland and H.T. Robinson, 1988. Silver nitrate increases embryo production in anther culture of Brussels sprouts. Ann. Bot. 62: 181–185.

    CAS  Google Scholar 

  • Biddington, N.L., R.A. Sutherland and H.T. Robinson, 1992. The effects of gibberellic acid, fluridone, abscisic acid and paclobutrazol on anther culture of Brussels sprouts. Plant Growth Regul. 11: 81–84.

    CAS  Google Scholar 

  • Blakeslee, A.F., F. Belling, M.E. Farnham and A.D. Bergner, 1922. A haploid mutant in the Jimson weed, Datura stramonium. Science 55: 646–647.

    PubMed  CAS  Google Scholar 

  • Brown, D.C.W., E.M. Watson and P.M. Pechan, 1993. Induction of desiccation tolerance in microspore-derived embryos of Brassica napus. In Vitro Cell Dev. Biol. 29: 113–118.

    Google Scholar 

  • Burnett, L., S. Yarrow and B. Huang, 1992. Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. oleifera. Plant Cell Rep. 11: 215–218.

    PubMed  CAS  Google Scholar 

  • Cardy, B.J., 1986. Production of anther-derived doubled haploids for breeding oilseed rape (Brassica napus L.). Ph.D. Thesis, University of Guelph, Guelph, Ontario.

    Google Scholar 

  • Cao, M.Q., F. Charlot and C. Doré, 1990. Embryogenèse et régénération de plantes de chou a choucroute (Brassica oleracea L. ssp. capitata) par culture in vitro de microspores isolées. C.R. Acad. Sci. Paris Série III, 310: 203–209.

    Google Scholar 

  • Charne, D.G. and W.D. Beversdorf, 1988. Improving microspore culture as a rapeseed breeding tool: The use of auxins and cytokinins in an induction medium. Can. J. Bot. 66: 1671–1675.

    CAS  Google Scholar 

  • Charne, D.G. and W.D. Beversdorf, 1991. Comparisons of agronomic and compositional traits in microspore-derived and conventional populations of spring Brassica napus. In: D.I. McGregor (Ed.), GCIRC, July 9–11, 1991, Saskatoon, Saskatchewan, pp. 64–69.

    Google Scholar 

  • Charne, D.G., P. Pukacki, L.S. Kott and W.D. Beversdorf, 1988. Embryogenesis following cryopreservation in isolated microspores of rapeseed (Brassica napus L.). Plant Cell Rep. 7: 407–409.

    Google Scholar 

  • Chen, J.L. and W.D. Beversdorf, 1990. A comparison of traditional and haploid-derived breeding populations of oilseed rape (Brassica napus L.) for fatty acid composition of the seed oil. Euphytica 51: 59–65.

    CAS  Google Scholar 

  • Chen, J.L. and W.D. Beversdorf, 1991. Fatty acid inheritance in microspore-derived populations of spring rapeseed (Brassica napus L.). Theor. Appl. Genet. 80: 465–469.

    Google Scholar 

  • Chen, J.L. and W.D. Beversdorf, 1992. Production of spontaneous diploid lines in isolated microspores following cryopreservation of spring rapeseed (Brassica napus L.). Plant Breed. 108: 324–327.

    Google Scholar 

  • Chen, Z.Z., S. Snyder, Z.G. Fan and W.H. Loh, 1993. High efficiency chromosome doubling by direct colchicine treatment of isolated microspores of Brassica napus. In: Crucifer Genetics Workshop. Saskatoon, Saskatoon, July 21–24, 1993, p. 12.

    Google Scholar 

  • Chiang, M.S., C. Frechette, G. Kuo, C. Chong and S.J. Delafield, 1985. Embryogenesis and haploid plant production from anther culture of cabbage (Brassica oleracea var. capitata). Can. J. Plant Sci. 65: 1033–1057.

    Google Scholar 

  • Chuong, P.V. and W.D. Beversdorf, 1985. High frequency embryogenesis through isolated microspore culture of B. napus and B. carinata Braun. Plant Sci. 39: 219–226.

    Google Scholar 

  • Chuong, P.V., C. Deslauriers, L.S. Ott and W.D. Beversdorf, 1988. Effects of donor genotype and bud sampling on microspore culture of Brassica napus. Can. J. Bot. 66: 1653–1657.

    Google Scholar 

  • Coventry, J., L. Kott and W.D. Beversdorf, 1988. Manual for Microspore Culture Technique for Brassica napus. Dept. of Crop Sci. Techn. Bull. OAC Publication 0489, Univ. of Guelph, Guelph.

    Google Scholar 

  • Deslauriers, C., A.D. Powell, K. Fuchs and K.P. Pauls, 1991. Flow cytometric characterization and sorting of cultured Brassica napus microspores. Biochim. Biophys. Acta 1091: 165–172.

    Google Scholar 

  • Doré, C., 1989. Obtention de plantes haploïdes de chou cabus (Brassica oleracea L. ssp. capitata) après culture in vitro d’ovules pollinisés par du pollen irradié. C.R. Acad. Sci. Paris 309 Série III: 729–734.

    Google Scholar 

  • Duijs, J.G., R.E. Voorrips, D.L. Visser and J.B.M. Custers, 1992. Microspore culture is successful in most crop types of Brassica oleracea L. Euphytica 60: 45–55.

    Google Scholar 

  • Dunwell, J.M. and L.M. Cornish, 1985. Influence of preculture variables on microspore embryo production in Brassica napus ssp. oleifera cv. Duplo. Ann. Bot. 56: 281–289.

    Google Scholar 

  • Dunwell, J.M., L.M. Cornish and A.G.L. Decourcel, 1985. Influence of genotype, plant growth, temperature and anther incubation temperature on microspore embryo production in Brassica napus ssp. oleifera. J. Exp. Bot. 36: 679–689.

    Google Scholar 

  • Dunwell, J.M. and N. Thurling, 1985. Role of sucrose in microspore embryo production in Brassica napus ssp. oleifera. J. Exptl. Bot. 36: 1478–1491.

    Google Scholar 

  • Eikenberry, E., 1993. Chromosome doubling of microspore-derived canola using trifluralin. In: Crucifer Genetics Workshop. Saskatoon, Saskatoon, July 21–24, 1993, Abstr. *54.

    Google Scholar 

  • Fabijanski, S., I. Altosaar and P.G. Arnison, 1987. Heat shock response of Brassica oleracea, L. (broccoli). J. Plant Physiol. 128: 29–38.

    CAS  Google Scholar 

  • Fabijanski, S., I. Altosaar and P.G. Arnison, 1992. Heat shock response during anther culture of broccoli (B. oleracea var. Italica). Plant Cell Tiss. Org. Cult. 26: 203–212.

    Google Scholar 

  • Fan, Z., K.C. Armstrong and W.A. Keller, 1988a. Development of microspores in vivo and in vitro in Brassica napus L. Protoplasma 147: 191–199.

    Google Scholar 

  • Fan, Z., L. Holbrook and W.A. Keller, 1988b. Isolation and enrichment of embryogenic microspores in Brassica napus L. by fractionation using percoll density gradient. In: 7th Intl. Rapeseed Congr. Poland, 1988, 92th Ed.

    Google Scholar 

  • Flores, H.E. and A.W. Galston, 1982. Polyamines and plant stress: activation of putrescine biosynthesis by osmotic shock. Science 217: 1259–1260.

    PubMed  CAS  Google Scholar 

  • Foisset, N., R. Delourme, M.O. Lucas and M. Renard, 1993. Segregation analysis of isozyme markers on isolated microspore-derived embryos in Brassica napus L. Plant Breed. 110: 315–322.

    CAS  Google Scholar 

  • Garrido, D., N. Eller, E. Heberle-Bors and O. Vicente, 1993. De novo transcription of specific mRNAs during the induction of tobacco pollen embryogenesis. Sex. Plant Reprod. 6: 40–45.

    Google Scholar 

  • George, L. and P.S. Rao, 1983. In vitro induction of pollen embryos and plantlets in Brassica juncea through anther culture. Plant Sci. Lett. 26: 111–116.

    Google Scholar 

  • Gland, A., R. Lichter and H.-G. Schweiger, 1988. Genetic and exogenous factors affecting embryogenesis in isolated microspore culture of Brassica napus L. J. Plant Physiol. 132: 613–617.

    Google Scholar 

  • Govil, S., S.B. Babbar and S.C. Gupta, 1986. Plant regeneration from in vitro cultured anthers of black mustard (Brassica nigra Koch). Plant Breed. 97: 64–71.

    Google Scholar 

  • Gresshoff, P.M. and C.H. Doy, 1972. Haploid Arabidopsis thaliana callus and plants from anther culture. Aust. J. Biol. Sci. 25: 259–264.

    Google Scholar 

  • Gruber, S. and G. Röbbelen, 1991. Fatty acid synthesis in microspore-derived embryoids of rapeseed (Brassica napus). In: D.I. McGregor (Ed.), GCIRC, July 9–11, 1991, Saskatoon, Saskatchewan, pp. 1818–1820.

    Google Scholar 

  • Guerrero, F. and J.E. Mullet, 1986. Increased abscisic acid biosynthesis during plant dehydration requires transcription. Plant Physiol. 80: 588–591.

    PubMed  CAS  Google Scholar 

  • Guha, S. and S.C. Maheshwari, 1964. In vitro production of embryos from anthers of Datura. Nature (London) 204: 297.

    Google Scholar 

  • Guha, S, and S.C. Maheshwari, 1966. Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature (London) 212: 97–98.

    Google Scholar 

  • Guy, C.L., J.L.A. Huber and S. Huber, 1992. Sucrose phosphate synthase and sucrose accumulation at low temperature. Plant Physiol. 100: 502–508.

    PubMed  CAS  Google Scholar 

  • Hamaoka, Y., Y. Fujita and S. Iwai, 1991. Effects of temperature on the mode of pollen development in anther culture of Brassica campestris. Physiol. Plant 82: 67–72.

    Google Scholar 

  • Hannig, A., 1993. Antimicrotubule agents for diploidizing haploid tissues in Brassica napus. In: Crucifer Genetics Workshop. Saskatoon, Saskatoon, July 21–24, 1993, Abstr. *57.

    Google Scholar 

  • Hansen, M. and K. Svinnset, 1993. Microspore culture of Swede (Brassica napus ssp. rapifera) and the effects of fresh and conditioned medium. Plant Cell Rep. 12: 496–500.

    PubMed  CAS  Google Scholar 

  • Hanson, A.D. and W.D. Hitz, 1982. Metabolic response of mesophytes to plant water deficits. Ann. Rev. Plant Physiol. 33: 163–203.

    Google Scholar 

  • Hause, B., G. Hause, P. Pechan and A.A.M. Van Lammeren, 1993. Cytoskeletal changes in induction of embryogenesis in microspore and pollen cultures of Brassica napus L. Cell Biol. Intl. 17: 153–168.

    Google Scholar 

  • Hetz, E. and O. Shieder, 1991. Direct embryogenesis and plant regeneration through microspore culture of Brassica nigra. Crucifer Newsl. 14, 15: 102–103.

    Google Scholar 

  • Hoekstra, F.A., L.M. Crowe and J.H. Crowe, 1989. Differential desiccation sensitivity of corn and Pennisetum pollen linked to their sucrose content. Plant Cell Environ. 12: 83–91.

    CAS  Google Scholar 

  • Hoekstra, F.A., J.H. Crowe and L.M. Crowe, 1991. Effect of sucrose on phase behaviour of membranes in intact pollen of Typha latifolia L. as measured with Fourier transform infrared spectroscopy. Plant Physiol. 97: 1073–1079.

    PubMed  CAS  Google Scholar 

  • Hoffmann, F., E. Thomas and G. Wenzel, 1982. Anther culture as a breeding tool in rape. II. Progeny analysis of androgenic lines and induced mutants from haploid cultures. Theor. Appl. Genet. 61: 225–232.

    Google Scholar 

  • Homer, M. and E. Street, 1978. Pollen dimorphism-origin and significance in pollen plant formation by anther culture. Ann. Bot. 42: 763.

    Google Scholar 

  • Huang, B., S. Bird, R. Kemble, D. Simmonds, W.A. Keller and B. Miki, 1990. Effects of culture density, conditioned medium and feeder cultures on microspore embryogenesis in Brassica napus L. cv. Topas. Plant Cell Rep. 8: 594–597.

    Google Scholar 

  • Huang, B. and W.A. Keller, 1989. Microspore culture technology. J. Tiss. Cult. Methods 12: 171–178.

    Google Scholar 

  • Iqbal, M.C.M. and C. Mollers, 1991. Selection for low glucosinolate content of rapeseed Brassica napus L. using haploid embryos from microspore culture. In: D.I. McGregor (Ed.), GCIRC, July 9–11, Saskatoon, Saskatchewan, pp. 187–190.

    Google Scholar 

  • Jain, R.K., U. Brune and W. Friedt, 1989. Plant regeneration from in vitro cultures of cotyledon explants and anthers of Sinapis alba and its implications on breeding of crucifers. Euphytica 43: 153–163.

    Google Scholar 

  • Jain, S.M., N. Bagga, N. Bhalla-Sarin, S. Guha-Mukherjee and S.K. Sopory, 1980. In vitro culture of anthers in Petunia hybrida and Brassica oleracea. In: P.S. Rao, M.R. Heble and M.S. Chadha (Eds.), Plant Tissue Culture, Genetic Manipulation and Somatic Hybridization of Plant Cells, pp. 85–92. Bhabha Atomic Research Centre, Bombay.

    Google Scholar 

  • Kameya, T. and K. Hinata, 1970. Induction of haploid plants from pollen grains of Brassica. Jpn. J. Breed. 20: 82–87.

    Google Scholar 

  • Keller, W.A. and K.C. Armstrong, 1977. Embryogenesis and plant regeneration in Brassica anther culture. Can. J. Bot. 55: 1383–1388.

    Google Scholar 

  • Keller, W.A. and K.C. Armstrong, 1978. High frequency production of microspore-derived plants from Brassica napus anther cultures. Z. Pfianzenzüchtg. 80: 100–108.

    Google Scholar 

  • Keller, W.A. and K.C. Armstrong, 1979. Stimulation and embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments. Theor. Appl. Genet. 55: 65–67.

    Google Scholar 

  • Keller, W.A. and K.C. Armstrong, 1981. Production of anther derived dihaploid plants in autotetraploid marrowstem kale (Brassica oleracea var acephala). Can. J. Genet. Cytol: 23: 259–265.

    Google Scholar 

  • Keller, W.A. and K.C. Armstrong, 1982. Production of haploids via anther culture in Brassica oleracea var. italica. Euphytica 32: 151–159.

    Google Scholar 

  • Keller, W.A., K.C. Armstrong and I.A. de la Roche, 1982. The production and utilization of microspore-derived haploids in Brassica crops. In: K.L. Giles and S.K. Sen (Eds.), Plant Cell Culture in Crop Improvement, pp. 169–183. Plenum Pub. Corp., New York.

    Google Scholar 

  • Keller, W.A., P.G. Arnison and B.K. Cardy, 1987. Haploids from gametophytic cells–recent development and future prospects. In: C.E. Green, D.A. Somers, W.P. Nackett and D.D. Biesboer (Eds.), Plant Tissue and Cell Culture, pp. 233–241. Allan R. Liss, New York.

    Google Scholar 

  • Keller, W.A., T. Rajhathy and J. Lacapra, 1975. In vitro production of plants from pollen in Brassica campestris. Can. J. Genet. Cytol. 17: 655–666.

    Google Scholar 

  • Keller, W.A. and G.R. Stringam, 1978. Production and utilization of microspore-derived plants. In: T.A. Thorpe (Ed.), Frontiers of Plant Tissue Culture, pp. 113–122. Intern. Assoc. Plant Tiss. Cult., Calgary.

    Google Scholar 

  • Kieffer, M., M.P. Fuller, J.E. Chauvin and A. Schlesser, 1993. Anther culture of kale (Brassica oleracea L. con var. acephala D.C. Alef.). Plant Cell Tiss. Org. Cult. 33: 303–313.

    Google Scholar 

  • Kimber, G. and R. Riley, 1963. Haploid angiosperms. Bot. Rev. 29: 480–531.

    Google Scholar 

  • Klimaszewska, K. and W.A. Keller, 1983. The production of haploids from Brassica hirta Moench (Sinapis alba) anther cultures. Z. Pflanzenphysiol. 109: 235–241.

    Google Scholar 

  • Komatsu, Y., 1936. Investigations on the progeny of haploid plants and embryo sac formation by diploid and haploid plants of Brassica napus. Proc. Crop Sci. Soc. Japan 8: 364–372.

    Google Scholar 

  • Kott, L.S. and W.D. Beversdorf, 1990. Enhanced plant regeneration from microspore-derived embryos of Brassica napus by chilling, partial desiccation and age selection. Plant Cell Tiss. Org. Cult. 23: 187–192.

    Google Scholar 

  • Kott, L.S., L. Polsoni and W.D. Beversdorf, 1988a. Cytological aspects of isolated microspore culture in Brassica napus. Can. J. Bot. 66: 1658–1664.

    Google Scholar 

  • Kott, L.S., L. Polsoni, B. Ellis and W.D. Beversdorf, 1988b. Autotoxicity in isolated microspore cultures of Brassica napus. Can. J. Bot. 66: 1665–1670.

    Google Scholar 

  • Kuriyama, H. and Y. Watanabe, 1955. Studies on the haploid plant of Brassica carinata. Ikushugaku Zasshi/Jpn. J. Breed. 5: 1–5.

    Google Scholar 

  • Kyo, M. and H. Harada, 1990. Specific phosphoproteins in the initial period of tobacco pollen embryogenesis. Planta 182: 58–63.

    PubMed  CAS  Google Scholar 

  • Leelavathi, S., V.S. Reddy and S.K. Sen, 1984. Somatic cell genetics in Brassica species. I. High frequency production of haploid plants in Brassica alba (L.) H.F. + T. Plant Cell Rep. 3: 102–105.

    Google Scholar 

  • Leelavathi, S., V.S. Reddy and S.K. Sen, 1987. Somatic cell genetic studies in Brassica species. II. Production of androgenetic haploid plants in Brassica nigra ( L.) Koch. Euphytica 36: 215–219.

    Google Scholar 

  • Lelu, M.-A. and H. Ballon, 1985. Obtention d’haploïdes par culture d’anthère de Brassica oleracea L. var. capitata et var. gemmifera. C.R. Acad. Sci. Paris 300, Série III, no. 2: 7176.

    Google Scholar 

  • Lichter, R., 1981. Anther culture of Brassica napus in a liquid culture medium. Z. Pflanzenphysiol. 103: 229–237.

    CAS  Google Scholar 

  • Lichter, R., 1982. Induction of haploid plants from isolated pollen of Brassica napus. Z. Pflanzenphysiol. 105: 427–434.

    Google Scholar 

  • Lichter, R., 1989. Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species. Plant Breed. 103: 119–123.

    Google Scholar 

  • Lichter, R., E. DeGroot, D. Fiebig, R. Schweiger and A. Gland, 1988. Glucosinolates determined by HPLC in the seeds of microspore-derived homozygous lines of rapeseed (Brassica napus L.). Plant Breed. 100: 269–221.

    Google Scholar 

  • Lillo, C. and M. Hansen, 1987. Anther culture of cabbage. Influence of growth temperature of donor plants and media composition on embryo yield and plant regeneration. Norwegian J. Agric. Sci. 1: 105–109.

    Google Scholar 

  • Liu, C.-M., Z.H. Xu and N.-H. Chua, 1993. Auxin polar transport is essential for establishment of bilateral symmetry during early plant embryogenesis. Plant Cell 5: 621–630.

    PubMed  CAS  Google Scholar 

  • Lo, K.-H. and K.P. Pauls, 1992. Plant growth environment effects on rapeseed microspore development and culture. Plant Physiol. 99: 468–472.

    PubMed  CAS  Google Scholar 

  • Loh, C.S. and D.S. Ingram, 1982. Production of haploid plants from anther cultures and secondary embryoids of winter oilseed rape, Brassica napus ssp. oleifera. New Phytol. 91: 507–516.

    Google Scholar 

  • Loh, C.S., D.S. Ingram and D.E. Hanke, 1983. Cytokinins and the regeneration of plantlets from secondary embryoids of winter oilseed rape, Brassica napus ssp. oleifera. New Phytol. 95: 349–358.

    CAS  Google Scholar 

  • Lott, C.S. and D.E. Haube, 1983. Cytokinins and the regeneration of plantlets from secondary embryoids of winter oilseed rape, Brassica napus ssp. oleifera. New Phytol. 95: 349–350.

    Google Scholar 

  • MacDonald, M.V., M.A. Hadwiger, F.N. Aslam and D.S. Ingram, 1988a. The enhancement of anther culture efficiency in Brassica napus ssp. oleifera Metzg. ( Sinsk) using low doses of gamma radiation. New Phytol. 110: 101–107.

    Google Scholar 

  • MacDonald, M.V., D.M. Newsholme and D.S. Ingram, 1988b. The biological effects of gamma irradiation on secondary embryoids of Brassica napus ssp. oleifera Metzg. ( Sinsk) winter oilseed rape. New Phytol. 10: 255–259.

    Google Scholar 

  • Maheshwari, S.C., A.K. Tyagi, K. Malhotra and S.K. Sopory, 1980. Induction of haploidy from pollen grains in angiosperms - the current status. Theor. Appl. Genet. 58: 193–206.

    Google Scholar 

  • Margale, E. and A.M. Chèvre, 1991. Factors affecting embryo production from microspore culture of Brassica nigra (Koch). Crucifer Newsl. 14, 15: 100–101.

    Google Scholar 

  • Mathias, R., 1988. An improved in vitro culture procedure for embryoids derived from isolated microspores of rape (Brassica napus L). Plant Breed. 100: 320–322.

    Google Scholar 

  • Mathias, R. and G. Röbbelen, 1991. Effective diploidization of microspore-derived haploids of rape (Brassica napus L.) by in vitro colchicine treatment. Plant Breed. 106: 82–84.

    CAS  Google Scholar 

  • Matsubayashi, M. and K. Kuranuki, 1975. Embryogenic response of the pollen to varied sucrose concentrations in anther culture. Sci. Rep. Fac. Agric. Kobe Univ. 11: 215–230.

    Google Scholar 

  • Melchers, G., 1972. Haploid higher plants for plant breeding. Z. Pflanzenzüchtg. 67: 21–32.

    Google Scholar 

  • Naleczynska, A. and T. Cegielska, 1991. Doubled haploid production and field experiments with homozygous lines of rapeseed. In: D.I. McGregor (Ed.), GCIRC, July 9–11, 1991, Saskatoon, Saskatchewan, pp. 1488–1491.

    Google Scholar 

  • Morinaga, T. and E. Fukushima, 1933. Karyological studies on a spontaneous haploid mutant of Brassica napella. Cytologia 4: 457–460.

    Google Scholar 

  • Nitsch, J.P., 1972. Haploid plants from pollen. Z. Pflanzenzüchtg. 67: 3–18.

    Google Scholar 

  • Nitzsche, W. and G. Wenzel, 1977. Haploids in Plant Breeding. In: Fortschr. Pflanzenzüchtg. 8. Parey, Berlin.

    Google Scholar 

  • Ockendon, D.J., 1984. Anther culture in Brussels sprouts (Brassica oleracea var. gemmifera). I. Embryo yields and plant regeneration. Ann. Appl. Biol. 105: 285–291.

    Google Scholar 

  • Ockendon, D.J., 1985. Anther culture in Brussels sprouts (Brassica oleracea var. gemmifera). II. Effect of genotype on embryo yields. Ann. Appl. Biol. 107: 101–104.

    Google Scholar 

  • Ockendon, D.J. and R. McClenaghan, 1993. Effect of silver nitrate and 2,4-D on anther culture of Brussels sprout (Brassica oleracea var. gemmifera). Plant Cell Tiss. Org. Cult. 32: 41–46.

    Google Scholar 

  • Ockendon, D.J. and R.A. Sutherland, 1987. Genetic and non-genetic factors affecting anther culture of Brussels sprout (Brassica oleracea var. gemmifera). Theor. Appl. Genet. 74: 566–570.

    Google Scholar 

  • Olsson, G. and A. Hagberg, 1955. Investigations on a haploid rape. Hereditas 41: 227–237.

    Google Scholar 

  • Orton, T.J. and M.A. Browers, 1985. Segregation of genetic markers among plants regenerated from cultured anthers of broccoli (Brassica oleracea var. italica). Theor. Appl. Genet. 69: 637–643.

    Google Scholar 

  • Osolnik, B., B. Bohancec and S. Jelaska, 1993. Stimulation of androgenesis in white cabbage (Brassica oleracea var. capitata) anthers by low temperature and anther dissection. Plant Cell Tiss. Org. Cult. 32: 241–246.

    Google Scholar 

  • Pan, Q.Y., G. Seguin-Swartz, R.K. Downey and G.F.W. Rakow, 1991. Number of microspores in immature and mature flowerbuds in Brassica species. In: D.I. McGregor (Ed.), 8th Intl. Rapeseed Congress, Vol. 6, pp. 1836–1839.

    Google Scholar 

  • Pandey, K.K., 1973. Theory and practice of induced androgenesis. New Phytol. 72: 1129–1140.

    Google Scholar 

  • Pearcy, R.W., 1978. Effect of growth temperature on the fatty acid composition of leaf lipids in Atriplex lentiformis ( Torr.) Wats. Plant Physiol. 61: 484–486.

    Google Scholar 

  • Pechan, P.M., D. Bartels, D.C.W. Brown and J. Schell, 1991. Messenger RNA and protein changes associated with induction of Brassica microspore embryogenesis. Planta 184: 161–165.

    PubMed  CAS  Google Scholar 

  • Pechan, P.M. and W.A. Keller, 1988. Identification of potentially embryogenic microspores in Brassica napus. Physiol. Plant. 74: 377–384.

    Google Scholar 

  • Pechan, P.M. and W.A. Keller, 1989. Induction of microspore embryogenesis in Brassica napu L. by gamma irradiation and ethanol stress. In Vitro Cell Devel. Biol. 25: 1073.

    Google Scholar 

  • Pechan, P.M., W.A. Keller, F. Mandy and M. Bergeron, 1988. Selection of Brassica napus L. embryogenic microspores by flow sorting. Plant Cell Rep. 7: 596–398.

    Google Scholar 

  • Phippen, C. and D.J. Ockendon, 1990. Genotype, plant, bud size and media factors affecting anther culture of cauliflower (Brassica oleracea var. botrytis). Theor. Appl. Genet. 79: 3338.

    Google Scholar 

  • Pierek, R.L.M., 1987. Test tube fertilization. In: R.L.M. Pierek (Ed.), In Vitro Culture of Higher Plants, pp. 234–257. Martinus Nijhoff Publishers, Dordrecht.

    Google Scholar 

  • Polsoni, L., L.S. Kott and W.D. Beversdorf, 1988. Large-scale microspore culture technique for mutation-selection studies in Brassica napus. Can. J. Bot. 66: 1681–1685.

    Google Scholar 

  • Prakash, S., 1973. Haploidy in Brassica nigra Koch. Euphytica 22: 613–614.

    Google Scholar 

  • Prakash, S., 1974. Haploid meiosis and origin of Brassica tournefortii Gouan. Euphytica 23: 591–595.

    Google Scholar 

  • Primard, C., C. Camilleri, M. Tepfer, C. Tourneur, M. Bonade-Bottino, A. Martin-Canadel, L. Jouanin, G. Pelletier and M. Renard, 1991. Towards in situ androgenesis in Brassica napus. In: D.I. McGregor (Ed.), GCIRC, July 9–11, 1991, Saskatoon, Saskatchewan, pp. 1128–1129.

    Google Scholar 

  • Ramanujam, S., 1941. A haploid plant in Toria (Brassica campestris). Proc. Indian Acad. Sci. B 14: 25–34.

    Google Scholar 

  • Rikin, A., J.W. Dillwith and D.K. Bergman, 1993. Correlation between the circadian rhythm of resistance to extreme temperatures and changes in fatty acid composition in cotton seedlings. Plant Physiol. 101: 31–36.

    PubMed  CAS  Google Scholar 

  • Renu-Arora, R., S.S. Bhojwani and R. Arora, 1988. Production of androgenic plants through pollen embryogenesis in anther cultures of Brassica carinata A. Draun. Biol. Plant. 30: 2529.

    Google Scholar 

  • Roulund, N., L. Hansted, S.B. Anderson and B. Farestveit, 1990. Effect of genotype, environment an carbohydrate on anther culture response in head cabbage (Brassica oleracea L. convar. capitata Alef.). Euphytica 49: 237–242.

    CAS  Google Scholar 

  • Sato, T., T. Nishio and M. Hirai, 1989a. Varietal differences in embryogenic ability in anther culture of Chinese cabbage (Brassica campestris ssp. pekinensis). Jpn. J. Breed. 39: 149–157.

    Google Scholar 

  • Sato, T., T. Nishio and M. Hirai, 1989b. Plant respiration from isolated microspore cultures of chinese cabbage (Brassica campestris ssp. pekinensis). Plant Cell Rep. 8: 486–488.

    Google Scholar 

  • Scarth, R., G. Sequin-Swartz and G.F.W. Rakow, 1991. Application of doubled haploidy to Brassica napus breeding. In: D.I. McGregor (Ed.), GCIRC, July 9–11, 1991, Saskatoon, Saskatchewan, pp. 1449–1453.

    Google Scholar 

  • Scholl, R.L. and J.A. Amos, 1980. Isolation of doubled-haploid plants through anther culture in Arabidopsis thaliana [analyzed genetically and cytologically]. Z. Pflanzenphysiol. 96: 407–414.

    Google Scholar 

  • Seguin-Swartz, G., D.S. Hutcheson and R.K. Downey, 1983. Anther culture in Brassica campestris. In: Proc. 6th Intl. Rapeseed Congr., Paris, pp. 246–251.

    Google Scholar 

  • Sharma, K.K. and S.S. Bhojwani, 1985. Microspore embryogenesis in anther cultures of two indian cultivars of Brassica juncea (L.) Czern. Plant Cell Tiss. Org. Cult. 4: 235–239.

    Google Scholar 

  • Shetty, K. and B.D. McKersie, 1993. Proline, thioproline and potassium mediated stimulation of somatic embryogenesis in alfalfa ( Medicago sativa L. ). Plant Sci. 88: 185–193.

    Google Scholar 

  • Siebel, J. and K.P. Pauls, 1989a. Inheritance of erucic acid content in populations of Brassica napus microspore-derived spontaneous diploids. Theor. Appl. Genet. 77: 489–494.

    CAS  Google Scholar 

  • Siebel, J. and K.P. Pauls, 1989b. A comparison of anther and microspore culture as a breeding tool in Brassica napus. Theor. Appl. Genet. 78: 473–479.

    Google Scholar 

  • Simmonds, D.H., C. Gervais and W.A. Keller, 1991. Embryogenesis from microspores of embryogenic and non-embryogenic lines of Brassica napus. In: D.I. McGregor (Ed.), 8th Intl. Rapeseed Congress Proc., July 9–13, Saskatoon, Saskatchewan, pp. 306–311.

    Google Scholar 

  • Sorvari, S., 1985. Production of haploids from anther culture in agriculturally valuable Brassica campestris L. cultures. Ann. Agr. Fenn. 24: 149–160.

    Google Scholar 

  • Stead, A.D., 1992. Pollination induced flower senescence. A. Review. Plant Growth Regul. 11: 13–20.

    Google Scholar 

  • Stringam, G.R. and R.K. Downey, 1973. Haploid frequencies in Brassica napus. Can. J. Plant. Sci. 53: 229–231.

    Google Scholar 

  • Stringam, G.R. and M.R. Thiagarajah, 1991. Effectiveness of selection for early flowering in F-2 populations of Brassica napus L. A comparison of doubled haploid and single seed descent methods. In: D.I. McGregor (Ed.), GCIRC, July 9–11, 1991, Saskatoon, Saskatchewan, pp. 70–75.

    Google Scholar 

  • Sunderland, N., 1971. Anther culture: a progress report. Sci. Prog. Oxford 59: 527–549.

    Google Scholar 

  • Swanson, E.B., 1990. Microspore culture in Brassica. In: J.W. Pollard and J.M. Walker (Eds.), Methods in Molecular Biology Vol. 6, Plant Cell and Tissue Culture, pp. 159–169. The Humana Press, Clifton, N.J., USA.

    Google Scholar 

  • Swanson, E.B., M.P. Coumans, G.L. Brown, J.D. Patel and W.D. Beversdorf, 1988. The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts. Plant Cell Rep. 7: 83–87.

    CAS  Google Scholar 

  • Swanson, E.B., M.P. Coumans, S.C. Wu, T. Barsby and W.D. Beversdorf, 1987. Efficient isolation of microspores and the production of microspore-derived embryos from Brassica napus. Plant Cell Rep. 6: 94–97.

    Google Scholar 

  • Takahata, Y., D.C.W. Brown and W.A. Keller, 1991. Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L. Euphytica 58: 51–55.

    Google Scholar 

  • Takahata, Y. and W.A. Keller, 1991. High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L. Plant Sci. 74: 235–242.

    Google Scholar 

  • Telmer, C.A., W. Newcomb and D.H. Simmonds, 1993. Microspore development in Brassica napus and the effect of high temperature on division in vivo and in vitro. Protoplasma 172: 154–165.

    Google Scholar 

  • Telmer, C.A., D.H. Simmonds and W. Newcomb, 1992. Determination of developmental stage to obtain high frequencies of embryogenic microspores in Brassica napus. Physiol. Plant 84: 417–424.

    Google Scholar 

  • Thomas, E. and G. Wenzel, 1975. Embryogenesis from microspores of Brassica napus. Z. Pflanzenzüchtg. 74: 79–81.

    Google Scholar 

  • Thompson, K.F., 1956. Production of haploid plants of marrow-stem kale. Nature (London) 178: 748.

    Google Scholar 

  • Thompson, K.F., 1969. Frequencies of haploids in spring oilseed rape (Brassica napus). Hereditas 24: 318–319.

    Google Scholar 

  • Thompson, K.F., 1974. Homozygous diploid lines from naturally occurring haploids. In: 4th Interul. Rapskongr Giessen, pp. 119–124.

    Google Scholar 

  • Thurling, N. and P.M. Chay, 1984. The influence of donor plant genotype and environment on production of multicellular microspores in cultured anthers of Brassica napus ssp. oleifera. Ann. Bot. 54: 681–695.

    Google Scholar 

  • Wenzel, G., 1980. Recent progress in microspore culture of crop plants. In: D.R. Davies and D.A. Opwood (Eds.), The Plant Genome, pp. 185–213. The John Innes Charity, Norwich.

    Google Scholar 

  • Yadav, R.C., N.R. Yadav, P.R. Kumar and D.R. Sharma, 1988. Differential androgenic response in Brassica juncea (L.) Czern and Coss. Cruciferae Newsl. No. 13: 76.

    Google Scholar 

  • Yang, Q., J.E. Chauvin and Y. Hervé, 1991. A study of factors affecting anther culture of cauliflower (Brassica oleracea var. botrytis). Plant Cell Tiss. Org. Cult. 28: 289–296.

    Google Scholar 

  • Yang, Q., J.E. Chauvin and Y. Hervé, Y. 1992. Obtention d’embryos androgénétiques par culture in vitro de boutons floraux chez le broccoli (Brassica oleracea var. italica). C.R. Acad. Sci. Paris 314 Série III: 145–152.

    Google Scholar 

  • Zaki, M. and H.G. Dickinson, 1990. Structural changes during the first divisions of embryos resulting from anther and microspore culture in Brassica napus. Protoplasma 156: 149–162.

    Google Scholar 

  • Zaki, M.A.M. and H.G. Dickinson, 1991. Microspore-derived embryos in Brassica: the significance of division symmetry in pollen mitosis I to embryogenic development. Sex. Plant Reprod. 4: 48–55.

    Google Scholar 

  • Zarsky, V., D. Garrido, L. Rihova, J. Tupy, O. Vicente and E. Heberle-Bors, 1992. Derepression of the cell cycle by starvation is involved in the induction of tobacco pollen embryogenesis. Sex. Plant Reprod. 5: 189–194.

    Google Scholar 

  • Zhang, W.J., G.H. Fang, K.X. Tang, Z.Q. Zhang and M.J. Yu, 1991. Plant regeneration from embryoids through isolated microspore culture in Brassica napus L. In: D.I. McGregor (Ed.), GCIRC, July 9–11, Saskatoon, Saskatchewan, pp. 1071–1074.

    Google Scholar 

  • Zhao, J.P. and D.H. Simmonds, 1992. In vitro manipulation of microspore embryogenesis to generate doubled haploid plants. In: 3rd Intern. Assoc. Plant Tiss. Cult. Workshop, June 1720, 1992, Abstr. #6.

    Google Scholar 

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Palmer, C.E., Keller, W.A., Arnison, P.G. (1996). Experimental haploidy in Brassica species. In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Current Plant Science and Biotechnology in Agriculture, vol 25. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1858-5_9

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