Summary
The kinetic parameters for growth and production of gibberellins by Gibberella fujikuroi, grown on liquid (LC), and solid substrate (SSC) cultivation, were compared. For the latter, organic (OSC) and inert (ISC) support cultures were investigated. Significantly higher biomass content and product yields were found for SSC, as compared to the LC system. However, similar specific productivities were found for LC and ISC, thereby suggesting that physical conditions are not necessarily a determining factor for the elevated product yields in SSC.
A significantly higher specific productivity and product yield for OSC, as compared with ISC, were observed. This could result from limited carbon catabolite repression, that might prevail in the former system. A further possibility could be the presence of an unknown compound in wheat bran, that strongly induced GA3 biosynthesis.
Major secreted gibberellins were GA3, GA1, GA13, GA4, GA7, GA9 and GA14, for all studied culture systems. A constant GA3/GA ratio was found throughout the cultivation for both SSC systems, thereby suggesting that the production of GA3 is a good indicator of the biosynthetic pathway expression for all gibberellins.
Resume
Nous avons comparé les cinétiques de croissance de Gibberella fujikuroi et de production de la gibberelline, pour deux techniques de culture: en milieu liquide (FL) et en milieu solide (FMS). Pour la FMS, les cultures ont été réalisées à la fois sur support organique (OSC) et sur support inerte (ISC). On observe en FMS une production de biomasse et des rendements de production nettement supérieurs à ceux obtenus en FL. Cependant la productivité spécifique mesurée est identique pour les deux techniques de fermentation: les caractéristiques physiques du support n’apparaissent pas comme le facteur déterminant les hauts rendements de production obtenus en FMS.
Nous avons également observé une productivité spécifique et un rendement de production plus élevé pour le milieu OSC que pour ISC. Cela peut s’expliquer par une répression catabolique limitée dans le premier milieu, par la présence dans le son de blé d’un composé inconnu fortement inducteur de la biosynthèse de la GA3.
Quelque soit la technique de fermentation utilisée, les gibberellines majoritairement produites sont les GA3, GA1, GA13, GA4, GA7, GA9 et GA14. On mesure un rapport GA3/GA3 constant au cours de la fermentation, pour les trois types de culture. On peut supposer que la production de GA3 est un bon indicateur de la voie anabolique des gibberellines.
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Agosin, E., Maureira, M., Biffani, V., Pérez, F. (1997). Production of gibberellins by solid substrate cultivation of Gibberella fujikuroi . In: Roussos, S., Lonsane, B.K., Raimbault, M., Viniegra-Gonzalez, G. (eds) Advances in Solid State Fermentation. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0661-2_29
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DOI: https://doi.org/10.1007/978-94-017-0661-2_29
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