Abstract
It is still common practice to find the most suitable expression system for a given protein by trial and error, as there are no clear rules available to make this decision on the basis of the properties of the desired protein. This work discusses several decision points between bacteria and yeasts, namely Escherichia coli and Pichia pastoris. Based on the expression of human trypsinogen 1, advantages and disadvantages of both expression systems are discussed, emphasizing yields and the efforts to obtain a functional product. Briefly, in E. coli a high yield of insoluble product was obtained which required substantial efforts for resolubilisation, whereas with P. pastoris a functional product was obtained at a comparatively low yield.
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© 2001 Springer Science+Business Media Dordrecht
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Hohenblum, H., Naschberger, S., Weik, R., Katinger, H., Mattanovich, D. (2001). Production of Recombinant Human Trypsinogen in Escherichia Coli and Pichia Pastoris . In: Merten, OW., et al. Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9749-4_25
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DOI: https://doi.org/10.1007/978-94-015-9749-4_25
Publisher Name: Springer, Dordrecht
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