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Prevention and Elimination of Contamination for in vitro Culture of Several Woody Species

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Pathogen and Microbial Contamination Management in Micropropagation

Part of the book series: Developments in Plant Pathology ((DIPP,volume 12))

Abstract

Microbial contamination is one of the most critical and frequently occurring problems in plant tissue culture. In previous experiments with several woody genera such as Castanea, [1–3] Ligustrum, Spirea, Syringa [4], Corylus, Populus, Salix [5], and others we worked out some successful techniques to establish contamination-free cultures. In most cases the available plant material was in very limited quantity, received from the breeding project of the American Chestnut Foundation [1,2], or as a part of a special plant exchange program with the University of Horticulture and Food, Budapest, Hungary [5] ; thus, it was extremely important not to lose any culture because of contamination problems.

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References

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A. C. Cassells

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© 1997 Springer Science+Business Media Dordrecht

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Szendrák, E., Read, P.E., Yang, G. (1997). Prevention and Elimination of Contamination for in vitro Culture of Several Woody Species. In: Cassells, A.C. (eds) Pathogen and Microbial Contamination Management in Micropropagation. Developments in Plant Pathology, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8951-2_29

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  • DOI: https://doi.org/10.1007/978-94-015-8951-2_29

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4932-2

  • Online ISBN: 978-94-015-8951-2

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