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UDP-Galactose: Diacylglycerol Galactosyltransferase in Cucumber Seedlings: Purification of the Enzyme and the Activation by Phosphatidic Acid

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Plant Lipid Metabolism

Abstract

UDP-galactose:diacylglycerol galactosyltransferase (UDGT) catalyzes the final step of the synthesis of monogalactosyl diacylglycerol (MGDG), a major glycohpid in plastid membrane. We found that the enzyme activity was strongly activated coincided with accumulation of galactolipids during germination of cucumber seedlings, suggesting that activation step of the UDGT was important in the development of chloroplast membrane [1]. However, mechanism of regulation of the UDGT activity during chloroplast formation remains unclear.

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References

  1. Ohta H, Shimojima M, Ookata K, Masuda T, Shioi Y, Takamiya K. A galactosyl-transferase is activated in light-mediated accumulation of galactolipids during development in cucumber seedlings. 1994;submitted.

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  2. Teucher, T and Heinz, E. Punfication of UDP-galactose:diacylglycerol galactosyl-transferase from chloroplast envelopes of spinach (Spinacia oleracea L.) Planta 1991;184:319–326.

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  4. Coves J, Joyard J, Douce R. Lipid requirement and kinetic studies of solubilized UDP-galactose:diacylglycerol galactosyltransferase activity from spinach chloroplast envelope membranes. Proc. Natl Acad .Sci. USA 1988;85:4966–4970.

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Authors and Affiliations

  1. Faculty of Bioscience & Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama 227, Japan

    Hiroyuki Ohta, Mie Shimojima, Takeshi Arai, Tatsuru Masuda, Yuzo Shioi & Ken-ichiro Takamiya

Authors
  1. Hiroyuki Ohta
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  2. Mie Shimojima
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  3. Takeshi Arai
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  4. Tatsuru Masuda
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  5. Yuzo Shioi
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  6. Ken-ichiro Takamiya
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Jean-Claude Kader Paul Mazliak

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© 1995 Springer Science+Business Media Dordrecht

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Ohta, H., Shimojima, M., Arai, T., Masuda, T., Shioi, Y., Takamiya, Ki. (1995). UDP-Galactose: Diacylglycerol Galactosyltransferase in Cucumber Seedlings: Purification of the Enzyme and the Activation by Phosphatidic Acid. In: Kader, JC., Mazliak, P. (eds) Plant Lipid Metabolism. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8394-7_42

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  • DOI: https://doi.org/10.1007/978-94-015-8394-7_42

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4498-3

  • Online ISBN: 978-94-015-8394-7

  • eBook Packages: Springer Book Archive

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