Abstract
Methods of separating motile human spermatozoa avoiding centrifugation have been described1–3. Ejaculated semen is deposited in contact with the culture medium, and then motile spermatozoa migrate upward into the supernatant, where they must be collected within 1 or 2 hours before their sedimentation. A simple new method which combines both the migration and the sedimentation phenomena has been developed4.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Lopata, A., Patullo, M.J., Chang, A. and James, B. (1976). A method for collecting motile spermatozoa from human semen. Fertil. Steril., 27, 677
Harris, S.J., Milligan, M.P., Masson, G.M. and Dennis, K.J. (1981). Improved separation of motile sperm in asthenospermia and its application to artificial insemination homologous. Fertil. Steril, 36, 219
Mortimer, D., Leslie, E.E., Kelly, R.W. and Templeton, A.A. (1982). Morphological selection of human spermatozoa in vivo and in vitro. J. Reprod. Fertil, 64, 391
Tea, N.T., Jondet, M. and Scholler, R. (1983). Procédé d’isolement de spermatozoides mobiles du sperme humain par la méthode de migration-sédimentation. Path. Biol 31, 688
Dominique, S., Cornier, E., Jondet, M. and Scholler, R. (1983). Fécondation in vitro et replacement: résultats préliminaires. Path. Biol 31, 693
Jondet, M. and Tea, N.T. (1980). Le spermogramme et son interprétation. In Scholler, R. (ed.) Hormonologie de la Stérilité, pp. 223–52. ( Paris: SEPE )
Eliasson, R., Johnsen, O. and Lindholmer, C. (1974). Effects of seminal plasma on some functional properties of human spermatozoa. In Mancini, R.E. and Martini, L. (eds.) Male Fertility and Sterility, pp. 107–21. ( London: Academic Press )
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 MTP Press Limited
About this chapter
Cite this chapter
Tea, N.T., Jondet, M., Scholler, R. (1984). A ‘migration-gravity sedimentation’ method for collecting motile spermatozoa from human semen. In: Harrison, R.F., Bonnar, J., Thompson, W. (eds) In Vitro Fertilizȧtion, Embryo Transfer and Early Pregnancy. Studies in Fertility and Sterility, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-8132-7_21
Download citation
DOI: https://doi.org/10.1007/978-94-011-8132-7_21
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-011-8134-1
Online ISBN: 978-94-011-8132-7
eBook Packages: Springer Book Archive