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Development of High Output Expression Vectors for Antibody Production in Mammalian Cells

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Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 9))

Abstract

The design of efficient expression vectors is a major component in the development of a high level production system for recombinant antibodies. The BKV enhancer/Adenovims major-late promoter (BKV/AdMLP) and human cytomegalovims immediate-early enhancer/promoter (CMV) were evaluated. The Adenovirus tripartite leader containing a chimeric intron (AdTpl-SS), immunoglobulin leader intron and J/C chimeric intron were aided and/or shifted. Cell lines COS-1, CV-1, 293S, CHO and NSO were used as hosts for transient assays. The secreted products were quantified by ELISA.

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References

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Author information

Authors and Affiliations

  1. Biomira Inc., 6100 Royalmount Ave., Montreal, QC, Canada, H4P 2R2

    Christian Gervais, Denis Paquette, Ann Burns-Tardif & Luis Martin

  2. Biomira inc., 9411 - 20 Ave., Edmonton, AB, Canada, T6N1H1

    Christian Gervais, Denis Paquette & Ann Burns-Tardif

  3. Biotechnology Research Institute/NRC, 6100 Royalmount Ave., Montreal, QC, Canada, H4P2R2

    Bernard Massie

Authors
  1. Christian Gervais
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  2. Denis Paquette
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  3. Ann Burns-Tardif
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  4. Luis Martin
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  5. Bernard Massie
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Editor information

Editors and Affiliations

  1. Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan

    K. Nagai  & M. Wachi  & 

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© 1998 Springer Science+Business Media Dordrecht

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Gervais, C., Paquette, D., Burns-Tardif, A., Martin, L., Massie, B. (1998). Development of High Output Expression Vectors for Antibody Production in Mammalian Cells. In: Nagai, K., Wachi, M. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5161-0_60

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  • DOI: https://doi.org/10.1007/978-94-011-5161-0_60

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6170-4

  • Online ISBN: 978-94-011-5161-0

  • eBook Packages: Springer Book Archive

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