Abstract
Initiated by the lack of oilseed rape cultivars (Brassica napus, genome AACC) resistant against Heterodera schachtii (beet cyst nematode, BCN) an introgression breeding programme has been started years ago. Oilradish genotypes (Raphanus sativus, genome RR) possessing a high degree of nematode resistance served as gene donors. As compared to all rapeseed genotypes being susceptible to cyst nematodes some highly resistant F1 hybrids (ACR), derived from the intergeneric cross B. napus x R. sativus, were detected. Allohexaploid plants (AACCRR) were produced by colchicine chromosome doubling and used in a backcross programme with summer type rapeseed as pollen donor. Selection of resistant individuals was performed by nematode infection tests in vivo. A progeny consisting of 51 BC2 plants derived from one highly resistant BC1 individual was tested for the degree of nematode resistance. Three resistant individuals were detected which are used to generate a BC3 progeny. In order to overcome the laborious testing procedure in vivo a molecular method for detecting plants containing a gene for nematode resistance would be very useful. Marker assisted selection would accelerate the process of breeding for a highly resistant substitution or translocation line. To find a molecular marker a segregating oilradish population is produced for a bulked segregant analysis. Based on putatively homologous DNA sequences of other resistance genes, primer pairs will be developed and tested to be applied as markers to accelerate selection in backcross generations without running nematode resistance tests in every generation.
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References
Baukloh, H. (1976) Untersuchung zur Wirtspflanzeneignung der Kruziferen gegenüber dem Rübennematode Heterodera schachtti (Schmidt), unter besonderer Berücksichtigung der Resistenzzüchtung. Diss. Georg-August-Universität, Göttingen, 73p.
Botella, M.A., Coleman, M.J., Hughes, D.E., Nishimura, MT., Jonesm J.D.G. & Somerville S.C. (1997) Map positions of 47 Arabidopsis sequences with sequence similarity to disease resistance genes. The Plant Journal 12: 1197–1212.
Brahm, L. (1997) Identifizierung molekularer Marker für die Resistenz der Sonnenblume (Helianthus annuus) gegen den Falschen Mehltau (Plasmopara halstedii) als Basis für eine markergestützte Selektion. Diss., Justus-Liebig-Universität Gießen, 96p.
BSA (1998) Beschreibende Sortenliste 1998. Bundessortenamt, Hannover/Germany.
Cai, D., Kleine, M., Kifle, S., Harloff, H.-J., Sandal, N.N., Marcker, K.A., Klein-Lankhorst, R.M., Salentijn, E.M.J., Lange, W., Stiekema, W.J., Wyss, U., Grundler, F.M.W. & Jung, C. (1997) Positional cloning of a gene for nematode resistance in sugar beet. Science 275: 832–834.
Gentzbittel, L., Mouzeyar, S., Badaoui, S., Mestries, E., Vear, F., DeLabrouhe, D.T. & Nicolas, P. (1998) Cloning of molecular markers for disease resistance in sunflower, Helianthus annuus L. TAG 96:519–525.
Michelmore, R.W, Paran, I., Kesseli, R.V. (1991). Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88: 9828–9832.
Ordon, F., Bauer, E., Dehmer, K.J., Graner, A. & Friedt, W. (1995). Identification of a RAPD-marker linked to the BaMMV/BaYMV resistance gene ym4. Barley Genet. Newsl. 24: 123–126.
Pan, D. (1997) Cytologische und genetische Untersuchungen zur Entwicklung neuer Rapsadditionslinien mit Nematodenresistenz aus Kreuzungen zwischen Brassica napus L. und Raphanus sativus L. Diss., Justus-Liebig-Universität Gießen, 98p.
Paulmann, W. & Röbbelen, G. (1988). Effective transfer of cytoplasmatic male sterility from radish (Raphanus sativus L.) to rape (Brassica napus L.). Plant Breeding 100: 299–309.
Rouppe Voort, J., Lindemann, W., Folkertsma, R., Hutten, R., Overmars, H., van der Vossen, E., Jacobsen, E. & Bakker J. (1998) A QTL for broad-spectrum resistance to cyst nematode species (Globodera spp.) maps to a resistance gene cluster in potato. TAG 96: 654–661.
Sandal, N.N., Salentijn, E.M.J., Kleine, M., Cai, D., Arens-de Reuver, M., Van Druten, M., De Bock, T.S.M., Lange, W., Stehen, P., Jung, C., Marcker, K., Stiekema, W.J. & Klein-Lankhorst, R.M. (1998) Backcrossing of nematode-resistant sugar beet: a second nematode resistance gene at the locus containing Hslpro-1? Mol. Breed. 3: 471–480
Savitsky, H. (1978) Nematode (Heterodera schachtii) resistance and meiosis in diploid plants from interspecific Beta vulgaris X B. prucumbens hybrids. Can. J. Genet. Cytol. 20: 177–186.
Scheffler, J.A., Sharpe, A.G., Schmidt, H., Sperling, P., Parkin, I.A.P., Lühs, W., Lydiate, D.J. & Heinz, E. (1997). Desaturase multigene families of Brassica napus arose through genome duplication. TAG 94:583–591.
Snowdon, R., Köhler, W., Friedt, W., Lühs, W. & Köhler, A. (1998). Detecting R-genome chromatin in nematode-resistant Brassica napus x Raphanus sativus hybrids. T. Lelley (Ed.), Current Topics in Plant Cytogenetics Related to Plant Improvement, 220–226. WUV-Universitätsverl., Wien.
Thierfelder, A. (1994). Genetische Untersuchungen für die züchterische Entwicklung neuer Rapssorten (Brassica napus L.) mit Resistenz gegen Nematoden (Heterodera schachtii Schm.). Diss., Justus Liebig-Universität Gießen, 106p.
Tokumasu, S. & Kato, M. (1988) Chromosomal and genetic structure of Brassicoraphanus related to seed fertility and the presentation of an instance of improvement of its fertility. Euphytica 39: 145–151.
Toxopeus, H. & Lubberts J.H. (1979). Breeding for resistance to the sugar beet nematode (Heterodera schachtii Schm.) in cruciferous crops. Proc. Eucarpia Cruciferae Conf. Wageningen (Post Conf Edition), p. 151.
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Voss, A., Lühs, W.W., Snowdon, R.J., Friedt, W. (1999). Development and molecular characterisation of nematode-resistant rapeseed (Brassica napus L.). In: Mugnozza, G.T.S., Porceddu, E., Pagnotta, M.A. (eds) Genetics and Breeding for Crop Quality and Resistance. Developments in Plant Breeding, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4475-9_22
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DOI: https://doi.org/10.1007/978-94-011-4475-9_22
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