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Plant Actin Isolation and Characterization

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Plant Molecular Biology Manual

Abstract

Monomeric actin (G-actin) is a small (42,000 Da) globular protein that polymerizes into higher order structures called microfilaments or filamentous actin (F- actin) (for a detailed review see [36]). These cytoskeletal polymers form a dynamic filament network in plant cells and support many fundamental processes, including cytoplasmic streaming, organelle positioning, cytokinesis, wound repair, responses to pathogen-attack, and cellular morphogenesis (for reviews see [23, 25, 26, 38]). Angiosperms contain large multigene families for actin and actin binding proteins (ABPs), and the biochemical and cell biological characterization of these components have received considerable attention in the past several years. A wealth of data is available for the motor molecule myosin and the small ABPs, profilin, and actin depolymerizing factor (reviewed in [3, 23, 26, 37]).

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Author information

Author notes

  1. Haiyun Ren

    Present address: Department of Biology, Beijing Normal University, Beijing, 100875, China

Authors and Affiliations

  1. 1392 Lilly Hall of Life Sciences, Dept. of Biological Sciences, Purdue University, West Lafayette, IN, 47907-1392, USA

    Christopher J. Staiger

  2. Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA

    Christopher J. Staiger, Bryan C. Gibbon & Haiyun Ren

Authors
  1. Christopher J. Staiger
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  2. Bryan C. Gibbon
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  3. Haiyun Ren
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Editors and Affiliations

  1. Purdue University, West Lafayette, Indiana, USA

    Stanton B. Gelvin

  2. Leiden University, Leiden, The Netherlands

    Robbert A. Schilperoort

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© 2000 Springer Science+Business Media Dordrecht

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Staiger, C.J., Gibbon, B.C., Ren, H. (2000). Plant Actin Isolation and Characterization. In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4217-5_5

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  • DOI: https://doi.org/10.1007/978-94-011-4217-5_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-7655-2

  • Online ISBN: 978-94-011-4217-5

  • eBook Packages: Springer Book Archive

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