Abstract
Biopharmaceutical products from mammalian cell culture are currently being produced in cGMP facilities using high density, immobilized, perfusion culture. Animal cells, including hybridomas, natural, and recombinant cells, are being immobilized in collagen microsponges. Process enhancement of the physical, chemical, and physiological properties of the culture optimize cell growth, productivity and product quality. The use of collagen microsponges (Verax Microspheres) enables high density cell culture (> 1.0 x 108 viable cells/mL matrix) at high perfusion rates and high cell specific productivities. Serum-free medium in combination with high density, perfused culture reduces production costs.
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© 1991 Springer Science+Business Media Dordrecht
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Runstadler, P.W., Young, M.W. (1991). GMP Production of Biopharmaceuticals Using High Density, Fluidized-Bed Cell Culture Technology. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_13
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DOI: https://doi.org/10.1007/978-94-011-3550-4_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5572-7
Online ISBN: 978-94-011-3550-4
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