Abstract
The absence of amyloid deposits in subcutaneous fat tissue has been reported in patients with amyloid deposits composed of beta-2-microglobulin (B2M) undergoing long-term hemodialysis (HD). We considered it of interest to ascertain, by combining abdominal fat aspiration with the immunoblot technique, if B2M is nevertheless deposited in non-amyloid form in the fat tissue. Twenty-seven patients undergoing regular HD for a median time of 11 yr (range 2-21) were evaluated; seventeen patients had demonstrated carpal tunnel amyloidosis (13 cases) or amyloid arthropathy (4 cases). The fat aspirate was divided into two portions: one for Congo red staining and microscopic analysis, the other for immunoblotting with anti-B2M and anti-P component antisera. Microscopic analysis detected definite amyloid deposits in only one patient, who turned out to have a previously undiagnosed AL amyloidosis. The immunoblot revealed trace amounts of B2M in 11 patients and no detectable B2M in 16. The search for P component was negative in all 11 patients investigated. Conclusion: our study confirms the absence of amyloid in abdominal fat aspirate in patients undergoing long-term HD, and demonstrates that B2M does not accumulate in this tissue.
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© 1991 Springer Science+Business Media Dordrecht
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Merlini, G. et al. (1991). Beta-2-Microglobulin Does Not Deposit in Abdominal Fat Tissue of Long-Term Hemodialysis Patients. In: Natvig, J.B., et al. Amyloid and Amyloidosis 1990. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3284-8_196
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DOI: https://doi.org/10.1007/978-94-011-3284-8_196
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