Abstract
Two different strategies are described for assessing effects of perinatal experiences on adult regional brain function. The first involves the use of the 14C-2DG autoradiographic method under challenge conditions. The approach is exemplified in a study of the effects of neonatal lesions and subsequent rearing conditions on regional brain activity in adulthood. Different profiles of regional 14C-2DG uptake were observed when animals were tested in the presence of a challenge stimulus compared to normal baseline conditions. A second approach to unmasking long-term alterations in regional brain activity involves the use of cytochrome oxidase histochemistry. The approach is illustrated in a study of the long-term effects of prenatal exposure to cocaine, where 14C-2DG autoradiography and quantitative cytochrome oxidase histochemistry were performed on the same brains. The latter technique revealed broader regional alterations than the former. Methodological aspects associated with paradigms involving comprehensive brain mapping in steady-state, chronic conditions are discussed.
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Nobrega, J.N. (1992). Brain Metabolic Mapping and Behaviour: Assessing the Effects of Early Developmental Experiences in Adult Animals. In: Gonzalez-Lima, F., Finkenstädt, T., Scheich, H. (eds) Advances in Metabolic Mapping Techniques for Brain Imaging of Behavioral and Learning Functions. NATO ASI Series, vol 68. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2712-7_4
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DOI: https://doi.org/10.1007/978-94-011-2712-7_4
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