Skip to main content

Laser Desorption Mass Spectrometry. Part II Performance and Applications of Matrix-Assisted Laser Desorption/Ionization of Large Biomolecules

  • Chapter
Mass Spectrometry in the Biological Sciences: A Tutorial

Part of the book series: NATO ASI Series ((ASIC,volume 353))

Abstract

Sample preparation and typical performance characteristics of ultraviolet and infrared matrix-assisted laser desorption/ionization mass spectrometry of large organic molecules are discussed.Examples of the application of the technique to the analysis of a variety different biopolymers are presented.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Karas, M., Ingendoh, A., Bahr, U. and Hillenkamp, F. (1989) UV-Laser Desorption/Ionization Mass Spectrometry of Femtomol Amounts of Large Proteins. Biomed. Environm. Mass Spectrometry, 18, 841–843

    Article  CAS  Google Scholar 

  2. Spengler, B., Kirsch, D., Kaufmann, R. (1991) Metastable Decay of Peptides and Proteins in Matrix-Assisted Laser Desorption Mass Spectrometry. Rapid Commun. Mass Spectrom., 5, 198–202

    Article  CAS  Google Scholar 

  3. Beavis, R., Chait, B. T. (1989) Factors Affecting the Ultraviolet Desorption of Proteins Rapid Commun, Mass Spectrom 3, 233

    Article  CAS  Google Scholar 

  4. Beavis, R., Chait, B. T. (1990) High-Accuracy Molecular Mass Determination of Proteins Using Matrix-Assisted Laser Desorption Mass Spectrometry. Anal. Chem. 62, 1836–1840

    Article  CAS  Google Scholar 

  5. Karas, M, Bahr, U., Ingendoh A. and Hillenkamp, F. (1989) Laserdesorptions-Massenspektrometrie von Proteinen mit Massen zwischen 100.000 und 250.000 Dalton. (in English edition: Laser Desorption/Ionization Mass Spectrometry of Proteins of Mass 100.000 to 250.000 Dalton) Angewandte Chemie 101, 805 – 806; Int. Ed. Engl. 28, 760

    Article  CAS  Google Scholar 

  6. Strupat, K. (1991) Untersuchungen zur Matrix-unterstützten ultravioletten Laser Desorptions/Ionisations Massenspektrometrie großer Moleküle: Einfluß der Probenpräparation bei der Benutzung unterschiedlicher Matrix-Wellenlängen Kombinationen Diploma Thesis, Universität Münster

    Google Scholar 

  7. Overberg, A., Karas, M., Bahr, U., Kaufmann R. and Hillenkamp, F. (1990) Matrix-Assisted Infrared (2.94 μm) Desorption/Ionization Mass Spectrometry of Large Biomolecules. Rapid Commun. Mass Spectrometry 4, 293–296

    Article  CAS  Google Scholar 

  8. Overberg, A., Karas M. and Hillenkamp, F. (1991) Matrix-Assisted laser Desorption of Large Biomolecules with a TEA-CO2-Laser. Rapid Commun. Mass Spectrometry. 5, 128–131

    Article  CAS  Google Scholar 

  9. Mecke, R. and Langenbucher, F. (1965) Infrared Spectra Heydon & Son Ltd., London,

    Google Scholar 

  10. Beavis, R. C. and Chait, B. T. (1989) Cinnamic Acid Derivatives as matrices for Ultraviolet Laser Desorption Mass Spectrometry of Proteins. Rapid. Commun. Mass Spectrom. 3, 432

    Article  CAS  Google Scholar 

  11. Beavis, R. and Chait, B. T. (1990) Rapid, Sensitive Analysis of Protein Mixtures by Mass Spectrometry Proc. Nat. Acad. Sc. , 87, 6873

    Article  CAS  Google Scholar 

  12. Karas, M. and Hillenkamp, F. (1988) Laser Desorption Ionization of Proteins with Molecular Mass Exceeding 10.000 Daltons Anal. Chem. 60, 2299–2301.

    Article  CAS  Google Scholar 

  13. Karas, M., Bahr, U., Ingendoh, A., Nordhoff, E., Stahl, B., Strupat, K. and Hillenkamp, F. (1990) Principles and Applications of Matrix-Assisted UV-Laser Desorption/ Ionization Mass Spectrometry Analytica Chimica Acta 241, 175–185

    Article  CAS  Google Scholar 

  14. Nordhoff, E.Untersuchungen zur Partiellen Sequenzanalyse von Proteinen und Polypeptiden durch enzymatischen Abbau und Matrix UV-Lasersdesorptions/Ionisations Massenspektrometrie. Diploma thesis, Universität Münster, 1991

    Google Scholar 

  15. Stahl, B., Steup, M., Karas, M. and Hillenkamp, F. (1991) Mass Spectrometry of Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization (LDI-MS), Anal. Chem., in press

    Google Scholar 

  16. Hillenkamp, F., Karas, M. (1990) Mass Spectrometry of peptides and Proteins by Matrix-Assisted Ultraviolet Laser Desorption/Ionization. In J. A. McCloskey (ed.), Methods in Enzymology, Vol. 193, Academic Press, Inc., New York, pg. 280–295.

    Google Scholar 

  17. Allmaier, G., Schmid, E. R., Hagspiel, K., Kubicek, C. P., Karas, M. and Hillenkamp,F. (1990) Strategy for the Characterization of the Glycoprotein Endoglucanase I, isolated from Trichoderrna reesei: Combination of Plasma Desorption and UV Laser Dersorption Time-of-FIight Mass Spectrometry. Analytica Chimica Acta 241, 321–327.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Overberg, A., Hassenbürger, A., Hillenkamp, F. (1992). Laser Desorption Mass Spectrometry. Part II Performance and Applications of Matrix-Assisted Laser Desorption/Ionization of Large Biomolecules. In: Gross, M.L. (eds) Mass Spectrometry in the Biological Sciences: A Tutorial. NATO ASI Series, vol 353. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2618-2_10

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-2618-2_10

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5157-6

  • Online ISBN: 978-94-011-2618-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics