Abstract
CHO cells have become increasingly important for recombinant gene expression, owing to their low rate of spontaneous transformation and biomanufacture of recombinant products that structurally and functionally resemble the native molecules. We recently developed a low protein (< 100 µg/ml), low endotoxin (<0.25 EU/ml) serum-free medium (CHO-S-SFM II) formulated to support the growth of CHO cells and the production of recombinant proteins in suspension culture. Both wild type and recombinant CHO cells were adapted, maintained, cryopreserved and recovered in CHO-S-SFM II. Cells cultured in this serum-free medium out-perform parallel cultures in serum-supplemented medium, reaching peak densities of 3–4 X 106 viable cells/ml and producing over 1.0 µg/m1 of recombinant human chorionic gonadotropin. CHO-S-SFM II demonstrated superior growth performance compared to four commercial serum-free media for CHO cells. A prototype powdered form of CHO-S-SFM Il exhibited performance equivalent to liquid medium. Serum-free medium eliminates problems associated with serum usage, such as lot-to-lot performance variability, presence of adventitious agents and fluctuations in price and availability. The low protein content of CHO-S-SFM II facilitates downstream processing of recombinant proteins and reduces final product cost. Additionally, the low endotoxin level of this medium reduces regulatory concerns for the production of therapeutic proteins.
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© 1993 Springer Science+Business Media Dordrecht
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Battista, P.J., Tilkins, M.L., Judd, D.A., Gorfien, S.F., Jayme, D.W. (1993). Chinese Hamster Ovary (CHO) Cell Growth and Recombinant Protein Production in Serum-Free Media. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_35
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DOI: https://doi.org/10.1007/978-94-011-2044-9_35
Publisher Name: Springer, Dordrecht
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