Abstract
Photosynthesis by isolated organelles, protoplasts and cells, prepared as described in Chapter 17, cannot easily be followed by infra-red gas analysis. 14CO2 fixation can provide invaluable information about products, but it does not lend itself to continuous measurement. For these reasons, routine measurements of photosynthesis are usually carried out with an oxygen electrode, which can easily detect changes of the order of 0.01 μmol in the oxygen content of a suspension. If necessary, 14CO2 fixation can be followed simultaneously in the same vessel by withdrawing samples with a micro-syringe at appropriate time intervals. These are injected immediately into HC1, and can be determined with a Geiger or scintillation counter, or subjected to chromatography.
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Walker, D.A. (1993). Polarographic measurement of oxygen. In: Hall, D.O., Scurlock, J.M.O., Bolhàr-Nordenkampf, H.R., Leegood, R.C., Long, S.P. (eds) Photosynthesis and Production in a Changing Environment. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1566-7_10
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DOI: https://doi.org/10.1007/978-94-011-1566-7_10
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