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Aniline Blue Staining of Human Spermatozoon Chromatin. Evaluation of Nuclear Maturation

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The Sperm Cell

Abstract

Cytochemical studies have shown that, in late spermatids, basic nucleoproteins, rich in lysine, are replaced by spermatozoon — specific nucleoproteins (3, 5). Acidic aniline blue is known to strongly stain the chromatin of early spermatids (7). Beyond these results, our observations demonstrate the variable ability of ejaculated human spermatozoa to stain with aniline blue.

We wish to thank Mrs. M.F. ALFONSI for her skilful technical assistance.

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References

  1. Black M.M. and Ansley H.R. 1966. Histone specificity revealed in ammoniacal silver staining. J. Histochem. Cytochem., 14, 177–181.

    Article  PubMed  CAS  Google Scholar 

  2. Courtens J.L. and Loir II. 1981a. Ultrastructural detection of basic nucleoproteins: alcoholic phosphotungstic acid does not bind to arginine residues. J. Ultrastruct. Res., 74, 322–326.

    Article  PubMed  CAS  Google Scholar 

  3. Courtens J.L. and Loir M. 1981b. A cytochemical study of nuclear changes in boar, bull, goat, mouse, rat and stallion spermatids. J. Ultrastruct. Res., 74, 327–340.

    Article  PubMed  CAS  Google Scholar 

  4. Dadoune J.P., Alfonsi M.F. and Terquem A. 1982. PTA cytochemical characterization of lysine residues in the nucleus of spermatids and ejaculated spermatozoa in man. First European Congress on Cell Biology, Paris 1982.

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  5. Gusse M. et Chevaillier Ph. 1978. Etude ultrastructurale et chimique de la chromatine au cours de la spermiogenese de la roussette Scyliorhinus caniculus (L.). Cytobiologie, 16, 421–443.

    PubMed  CAS  Google Scholar 

  6. Issidorides M.R. and Katsorchis T. 1981. Dispersed and compact chromatin demonstrated with a new EM method: phosphotungstic acid hematoxylin block-staining. Histochemistry, 73, 21–31.

    Article  PubMed  CAS  Google Scholar 

  7. Mc Kay R.B. 1962. An investigation of the anomalous staining of chromatin by the acid dyes, methyl blue and aniline blue. Quart. J. micr. Sci., 103, 519–530.

    CAS  Google Scholar 

  8. Notenboom C.D. Van de Veerdonk F.C.G. and Van de Kamer J.C. 1967. A fluorescent modification of the Sakaguchi reaction on arginine. Histochemie, 9, 117–121.

    Article  PubMed  CAS  Google Scholar 

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© 1983 Martinus Nijhoff Publishers, The Hague

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Terquem, A., Dadoune, J.P. (1983). Aniline Blue Staining of Human Spermatozoon Chromatin. Evaluation of Nuclear Maturation. In: André, J. (eds) The Sperm Cell. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7675-7_46

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  • DOI: https://doi.org/10.1007/978-94-009-7675-7_46

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-7677-1

  • Online ISBN: 978-94-009-7675-7

  • eBook Packages: Springer Book Archive

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